This experiment is a qualitative experiment which shows if an individual has a specific dimorphic Alu element. We used a process called Polymerase Chain Reaction (PCR) to identify this Alu element.
Knowing whether or not an individual possesses a certain gene can be very important in scientific research. Do to this importance PCR allows scientist to locate these Alu’s relatively easy. Our variables in this experiment were the hairs of the test subject, the lysis solution, the time of the water baths, the time of vortexing, whether or not the reaction pellet dissolved, the microcentrifuge, the thermal cycler, the primer solution, the automatic cycler, the agarose gel, whether or not the electrophoresis apparatus was set up and used properly, the loading of the DNA samples in the wells of the gel bed, proper staining of the DNA, and finally the operator error. Our hypothesis is 50 % of the subjects DNA samples contain the genotype. Our null hypothesis is that the genotype is there by chance and there is no genotype in these samples.
Experiment/ Methods 1. Obtain three to four hairs containing a sheath, a barrel-shaped structure (often white in color) encircling the shaft near the base of the hair. If necessary, sheaths can be cut from the remainder of the hair shaft. 2. Place the hairs in the bottom of a 1.5 ml screw-cap tube. 3. Obtain the lysis solution from your instructor. 4. Mix the lysis solution by vortexing or pipeting up and down. Before the chelating agent settles, quickly remove 150µl and add it to the tube containing the hair. 5. Make sure the hair sheaths are completely submerged in solution and are not stuck on the sides of the tube. 6. Place the tube in a 56°C water bath for 15 minutes. 7. Remove the tube from the water bath and allow it to cool for 30 seconds. 8. Vortex the tube for 15 seconds. 9. Check again that the hair sheaths are completely