Preview

Alu-Human Dna Typing Through Pcr

Good Essays
Open Document
Open Document
1376 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Alu-Human Dna Typing Through Pcr
ALU-HUMAN DNA TYPING THROUGH PCR

Abstract
This experiment is a qualitative experiment which shows if an individual has a specific dimorphic Alu element. We used a process called Polymerase Chain Reaction (PCR) to identify this Alu element.
Introduction
Knowing whether or not an individual possesses a certain gene can be very important in scientific research. Do to this importance PCR allows scientist to locate these Alu’s relatively easy. Our variables in this experiment were the hairs of the test subject, the lysis solution, the time of the water baths, the time of vortexing, whether or not the reaction pellet dissolved, the microcentrifuge, the thermal cycler, the primer solution, the automatic cycler, the agarose gel, whether or not the electrophoresis apparatus was set up and used properly, the loading of the DNA samples in the wells of the gel bed, proper staining of the DNA, and finally the operator error. Our hypothesis is 50 % of the subjects DNA samples contain the genotype. Our null hypothesis is that the genotype is there by chance and there is no genotype in these samples.
Experiment/ Methods 1. Obtain three to four hairs containing a sheath, a barrel-shaped structure (often white in color) encircling the shaft near the base of the hair. If necessary, sheaths can be cut from the remainder of the hair shaft. 2. Place the hairs in the bottom of a 1.5 ml screw-cap tube. 3. Obtain the lysis solution from your instructor. 4. Mix the lysis solution by vortexing or pipeting up and down. Before the chelating agent settles, quickly remove 150µl and add it to the tube containing the hair. 5. Make sure the hair sheaths are completely submerged in solution and are not stuck on the sides of the tube. 6. Place the tube in a 56°C water bath for 15 minutes. 7. Remove the tube from the water bath and allow it to cool for 30 seconds. 8. Vortex the tube for 15 seconds. 9. Check again that the hair sheaths are completely

You May Also Find These Documents Helpful

  • Satisfactory Essays

    3. Stretch the steel wool out (don’t wad it up) and gently push it into the bottom of a test tube.…

    • 890 Words
    • 5 Pages
    Satisfactory Essays
  • Better Essays

    pGLO Lab Report

    • 835 Words
    • 4 Pages

    The plasmid pGLO contains an antibiotic-resistance gene, ampR, and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus, transformed E. coli cells containing ampicillin-resistance plasmids can easily be selected simply growing the bacteria in the presence of ampicillin-only the transformed cells survive. The ara control region regulates GFP expression by the addition of arabinose, so the GFP gene can be turned on and off by including or omitting arabinose from the culture medium.…

    • 835 Words
    • 4 Pages
    Better Essays
  • Powerful Essays

    Use a sterile plastic inoculating loop to transfer one large colony of E. coli cells from the starter plate to the +plasmid tube.…

    • 842 Words
    • 4 Pages
    Powerful Essays
  • Satisfactory Essays

    Bath Bombs

    • 293 Words
    • 2 Pages

    4. The test tube was carefully returned to the lab bench and placed in a hot-water bath. After about 5…

    • 293 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Paradichlorobenzene Lab

    • 923 Words
    • 4 Pages

    12. Lower the test tube into the warm water until all of the Paradichlorobenzene is under water, then clamp the test tube in place.…

    • 923 Words
    • 4 Pages
    Good Essays
  • Better Essays

    Population Genetics

    • 1551 Words
    • 7 Pages

    The purpose of the population genetics lab was to determine the presence of the Alu element in human genome using polymerase chain reaction. Polymerase chain reaction was used to genotype a sample population. PCR is used to produce multiple copies of particular sequences of DNA (Vliet 1993). Polymerase chain reaction is based on the analysis of mitochondrial DNA sequences which also been used in the analysis of complex DNA samples. The advantage of mitochondrial based DNA analyses was that there are many mitochondria per cell and many mitochondrial DNA molecules within each mitochondria which makes mitochondrial DNA a naturally amplified source of genetic variation. Alu elements are the most abundant repetitive elements. Alu elements are short interspersed elements that amplify in primate genomes through a process termed retroposition (Batzer 1994). Alu elements comprise an estimated 5% of the human genome (Vliet 1993). Alu are inserted at specific chromosome locations and does not appear to…

    • 1551 Words
    • 7 Pages
    Better Essays
  • Good Essays

    Chemical Relaxer

    • 526 Words
    • 3 Pages

    Apply the relaxer ½ inch from the scalp, and spread ½ inch of the hair ends. First apply the relaxer to the top side of the hair. Then, raise the section and apply the relaxer underneath. Gently lay the hair up out of the way. Moving in clockwise direction, cover each section of the head in the same manner. Then, go back over the head in the same order, applying additional relaxing cream, if necessary, and spread the relaxer close to scalp and up to the ends. Smoothing the cream through the hair not only spreads the cream, but also gently stretches the hair into a straight pattern. While spreading the relaxer, pay close attention to its action by stretching the strand to see how fast the natural curl is being removed. Rinsing out the relaxer when the hair has been successfully straightened, rinse the relaxer out rapidly and thoroughly. The water should be warm, not hot. If the water is too hot, it may burn and cause discomfort because of the sensitive condition of the scalp. The force of the rinse water should be used to remove the relaxer and avoid tangling the hair. Part the hair with your fingers to make sure the relaxer is completely removed, its chemical action continues on the hair. The…

    • 526 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Chelex Lab Report

    • 1143 Words
    • 5 Pages

    Chelex Extraction and PCR Amplification of the Amelogenin Gene For The Purpose of Gender Determination. Abstract The amelogenin gene is a protein involved in the development of the enamel matrix. It is located on both the X and Y chromosomes in humans and can be used as a way to distinguish between males and females.…

    • 1143 Words
    • 5 Pages
    Better Essays
  • Good Essays

    2-Close the tube securely with a bung and place the tube on its side in the petri dish to stop it rolling away. Place the dish on the white tile to help you see the colour change…

    • 1161 Words
    • 5 Pages
    Good Essays
  • Good Essays

    E. Colo Lab Report

    • 691 Words
    • 3 Pages

    7. HEAT SHOCK; Transfer all the tubes to a water bath preheated to 37˚C, and allow them to sit in the water bath for 5…

    • 691 Words
    • 3 Pages
    Good Essays
  • Good Essays

    4. Place all the cylinders into a Petri dish and cover to prevent drying out.…

    • 336 Words
    • 2 Pages
    Good Essays
  • Good Essays

    5. Place 2 test tubes in each water bath and allow for milk to raise to temperature of water bath.…

    • 692 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Dna Extraction Lab Report

    • 666 Words
    • 3 Pages

    e) confirming the presence of the DNA: the concentration and quality of the DNA have to be determined; this can be…

    • 666 Words
    • 3 Pages
    Good Essays
  • Good Essays

    2. PCR has the ability to isolate specific DNA sequences with the use of primers. This is done by denaturing the DNA (at 95o C) so it is able to anneal to the primers that specify a fragment to be amplified (Mullis et. at. 1986). These primes anneal to a specific sequence of DNA in order to amplify this desired sequence.…

    • 524 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Label 4 test tubes and fill each with 5mL of 3.0% hydrogen peroxide and 5mL of water…

    • 808 Words
    • 4 Pages
    Good Essays