Age-Associated Decreased Activities of Mitochondrial Electron Transport Chain Complexes in Heart and Skeletal Muscle: Role of L-Carnitine
Primary Source Review #1
Title: Age-associated decreased activities of mitochondrial electron transport chain complexes in heart and skeletal muscle: role of L-carnitine
Authors: Kumaran S., Subathra M., Balu M., Panneerselvam C.
Journal: Chemo-Biological Interactions Vol. 148 Pgs. 11-18
Date: 2004
Instructions: You may work together on this assignment and turn it in as a group or individually. You will need to use resources such as the internet in addition to your textbook and the article to answer all the questions below. Allow enough time to read and thoroughly answer each question – I will be reading your answers very closely!!
General Questions
1. What is the purpose of this study?
The purpose of this study is to find out the effect of L-carnitine on the activities of electron transport chain in young and aged rats.
2. What are the main conclusions of this study?
The conclusion is that carnitine may prevents free radicals mediated mitochondrial membrane damages and increases the electron flow through the electron transport chain and thereby increasing energy production in aged rats.
3. How could this study be improved? This is a very open-ended question; just do the best you can.
In this study in activity assays for complex they measure data in different amount of time. For the complex 1 and 3 the initial rate was monitored for one minute, for the complex 2 for 3 minutes, while for complex 4 it was monitored for 15 seconds.
Also, they use a different length for monitoring. For the complex 1 it was 340 nm, for the complex 2 it was 600 nm and for the complexes 3 and 4 it was 550 nm.
In the study is not mentioned duration of experiment for control group.
In the results authors did not giving data about all experimental groups.
Data used in conclusion are not the same with a data in figures
4. Define the following terms:
• Senescence: the organic process of growing older and showing the effect of increasing age.
•
Title: Age-associated decreased activities of mitochondrial electron transport chain complexes in heart and skeletal muscle: role of L-carnitine
Authors: Kumaran S., Subathra M., Balu M., Panneerselvam C.
Journal: Chemo-Biological Interactions Vol. 148 Pgs. 11-18
Date: 2004
Instructions: You may work together on this assignment and turn it in as a group or individually. You will need to use resources such as the internet in addition to your textbook and the article to answer all the questions below. Allow enough time to read and thoroughly answer each question – I will be reading your answers very closely!!
General Questions
1. What is the purpose of this study?
The purpose of this study is to find out the effect of L-carnitine on the activities of electron transport chain in young and aged rats.
2. What are the main conclusions of this study?
The conclusion is that carnitine may prevents free radicals mediated mitochondrial membrane damages and increases the electron flow through the electron transport chain and thereby increasing energy production in aged rats.
3. How could this study be improved? This is a very open-ended question; just do the best you can.
In this study in activity assays for complex they measure data in different amount of time. For the complex 1 and 3 the initial rate was monitored for one minute, for the complex 2 for 3 minutes, while for complex 4 it was monitored for 15 seconds.
Also, they use a different length for monitoring. For the complex 1 it was 340 nm, for the complex 2 it was 600 nm and for the complexes 3 and 4 it was 550 nm.
In the study is not mentioned duration of experiment for control group.
In the results authors did not giving data about all experimental groups.
Data used in conclusion are not the same with a data in figures
4. Define the following terms:
• Senescence: the organic process of growing older and showing the effect of increasing age.
•