Agars

Topics: Escherichia coli, Coliform bacteria, Bacteria Pages: 3 (276 words) Published: September 17, 2013

VRBA

|Appearance of Colonies |Microorganisms | |Red, surrounded by reddish precipitation zones, diameter1- 2 mm |Lactose-positive Enterobacteriaceae: coliform bacteria, E. coli | |Pink, pin-point colonies |Enterococci, possibly Klebsiella | |Colorless |Lactose-negative Enterobacteriaceae |

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E. coli: dark-blue to violet colonies (Salmon-GAL and X-glucuronide reaction).

Total coliforms: salmon to red colonies (Salmon-GAL reaction) and dark-blue to violet colonies (E. coli).

Other Gram-negatives: colorless colonies, except for some organisms which possess ß-D-glucuronidase activity. These colonies appear light-blue to turquoise.

In order to confirm E. coli, coat the dark-blue to violet colonies with a drop of KOVACS' indole reagent. If the reagent turns to a cherry-red color after some seconds, a positive indole formation confirms the presence of E. coli.

E. coli in Macconkey Agar

MACCONKEY AGAR

|Appearance of Colonies |Microorganisms | |Colorless, translucent |Salmonella, Shigella, others | |Large, red, surrounded by turbid zones |Escherichia coli | |Large, pink, mucoid |Enterobecter, Klebsiella | |Very small, opaque, isolated colonies |Enterococci, Staphylococci, and others |

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