Action Potentials: Experiments and Results

Topics: Action potential, Neuron, Membrane potential Pages: 4 (772 words) Published: April 14, 2015
LABORATORY REPORT
Activity:

Action Potentials

Name:

Caitlin Magnacca

Instructor:
Date:

03.19.2015

Predictions
Exceeding threshold depolarization at the trigger zone______the likelihood of generation of an action potential. increases
Action potential amplitude
decreases with distance
Increasing frequency of stimulation to the trigger zone
increases number of action potentials

Materials and Methods
Experiment 1: Effect of Stimulus Strength on Action Potential Generation Dependent Variable
membrane potential
Independent Variable
stimulus strength (voltage)
Controlled Variables
frequency of stimulation, type of neuron

Experiment 2: Effect of Frequency of Stimulation on Action Potential Generation Dependent Variable
membrane potential
Independent Variable
frequency of stimulation
Controlled Variables
stimulus strength (voltage), type of neuron

1. Which part of the neuron was stimulated?

2. Where was membrane potential measured?

3. What was used to measure membrane potential?

Results
Table 3: Membrane Potentials at Different Stimulation Voltages, by Location. a. Values of maximal depolarization of membrane potential (mV) at different stimulation voltages, by location. Location

Axon hillock
Axon

0 V (no stimulation )
-68.5
-68.5

2V
-63.6
-72.4

b. Action Potential Generation.
Laboratory Report/ Caitlin Magnacca/ Action Potentials/ / 03.19.2015/ Page [1] of [4]

Stimulation Voltage
4V
-56.1
-63.8

6V
31.4
31.7

8V
30.9
-68.5

Location

Action
potential
generated?
Difference in
action
potential
amplitude
with
distance

Stimulation voltage
4V
6V

0 V (no
stimulation )
no

2V

8V

no

no

yes

yes

0

8.8

7.7

-0.3

99.4

Graph 1. Maximal depolarization of membrane potential at axon hillock and axon after different stimulation voltages.

Resting membrane potential = -70 mV.
1. What was the resting membrane potential (no stimulation) recorded in...
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