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ABO Blood Test to Determine Paternity

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ABO Blood Test to Determine Paternity
ABO Blood Test to Determine Paternity

Joshua Lee
Paige McNeal
Hazel Conway
February 12, 2013
Dr. Bose
Biology 240-33 Lab

Abstract

The ABO blood test was used in this lab to test which of the three fathers is the blood father of Andrea. The blood test that is closely related to the blood type of Andrea is the respective blood father. Each blood sample was mixed with synthetic anti-A, anti-B, and anti-Rh to test for clotting or not. Father #3 and Andrea were the only subjects tested with Rh positive with all other subjects Rh negative, resulting in Father #3 as the blood father.

Introduction
Table 1: Possible Child Blood Types
Table 1: Possible Child Blood Types

The purpose of this lab is to determine which father is the blood father of the child. The mother, Andrea, and three other fathers were tested by their blood type and Rh factor to determine the blood father. The ABO blood test determines the blood type of subjects by the alleles IA, IB, and i. The blood alleles IA and IB are codominant over i, meaning both of the alleles are dominant over the recessive i. The antigen with the Rh factor is determined by D as dominant or d as recessive, with the Rh positive factor as dominant and the Rh negative factor as recessive. In the ABO blood test, synthetic blood samples and synthetic serum of Anti-A, Anti-B, and Anti-Rh are mixed respectively into a blood typing slide. If agglutination occurs as the samples are mixed, then the subject’s blood type is the respective A, B, or Rh factor. The father with the most closely related alleles with Andrea is the blood father by testing June, the mother, Andrea, and all the fathers.

Methods

The container vials with synthetic blood samples each are respectively the mother, the child, Father #1, Father #2, and Father #3. Each of the respective blood samples are used once ever round in the experiment. One of the blood samples is dropped into the blood typing slide under three labels; A, B, and Rh. After


References: Q, R. (2010, January 5). Paternity testing using ABO blood types is impossible. Retrieved 2010, from Boston Paternity; The DNA Solution Blog: http://www.bostonpaternity.com/blog/paternity-testing-using-abo-blood-types-is-impossible/ Biology 240 Lab Handout, Lab 4: Multiple alleles and the ABO blood type
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