20121107 Oxygen And Air Nanobubble Wate

Oxygen and Air Nanobubble Water Solution Promote the
Growth of Plants, Fishes, and Mice
Kosuke Ebina1*, Kenrin Shi1, Makoto Hirao2, Jun Hashimoto3, Yoshitaka Kawato1, Shoichi Kaneshiro1,
Tokimitsu Morimoto1, Kota Koizumi1, Hideki Yoshikawa1
1 Department of Orthopaedic Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan, 2 Department of Orthopaedic Surgery, National Hospital
Organization, Osaka Minami Medical Center, Kawachinagano, Osaka, Japan, 3 Department of Immunology, National Hospital Organization, Osaka Minami Medical Center,
Kawachinagano, Osaka, Japan

Abstract
Nanobubbles (,200 nm in diameter) have several unique properties such as long lifetime in liquid owing to its negatively charged surface, and its high gas solubility into the liquid owing to its high internal pressure. They are used in variety of fields including diagnostic aids and drug delivery, while there are no reports assessing their effects on the growth of lives.
Nanobubbles of air or oxygen gas were generated using a nanobubble aerator (BUVITAS; Ligaric Company Limited, Osaka,
Japan). Brassica campestris were cultured hydroponically for 4 weeks within air-nanobubble water or within normal water.
Sweetfish (for 3 weeks) and rainbow trout (for 6 weeks) were kept either within air-nanobubble water or within normal water. Finally, 5 week-old male DBA1/J mice were bred with normal free-chaw and free-drinking either of oxygennanobubble water or of normal water for 12 weeks. Oxygen-nanobubble significantly increased the dissolved oxygen concentration of water as well as concentration/size of nanobubbles which were relatively stable for 70 days. Airnanobubble water significantly promoted the height (19.1 vs. 16.7 cm; P,0.05), length of leaves (24.4 vs. 22.4 cm; P,0.01), and aerial fresh weight (27.3 vs. 20.3 g; P,0.01) of Brassica campestris compared to normal water. Total weight of sweetfish increased from 3.0 to 6.4 kg in normal water, whereas it increased from 3.0 to 10.2

References: 1. Agarwal A, Ng WJ, Liu Y (2011) Principle and applications of microbubble and nanobubble technology for water treatment 2. Matsuki N, Ichiba S, Ishikawa T, Nagano O, Takeda M, et al. (2012) Blood oxygenation using microbubble suspensions 3. Takahashi M, Chiba K, Li P (2007) Free-radical generation from collapsing microbubbles in the absence of a dynamic stimulus 4. Eriksson JC, Ljunggren S (1999) On the mechanically unstable free energy minimum of a gas bubble which is submerged in water and adheres to a 5. Ljunggren S, Eriksson JC (1997) The lifetime of a colloid-sized gas bubble in water and the cause of the hydrophobic attraction 6. Li P, Takahashi M, Chiba K (2009) Enhanced free-radical generation by shrinking microbubbles using a copper catalyst 7. Bitterman H (2009) Bench-to-bedside review: oxygen as a drug. Crit Care 13: 205. 8. Abdelsalam M, Cheifetz IM (2010) Goal-directed therapy for severely hypoxic patients with acute respiratory distress syndrome: permissive hypoxemia 9. Guo S, Dipietro LA (2010) Factors affecting wound healing. J Dent Res 89: 219– 229. 10. Barbosa FT, Juca MJ, Castro AA, Duarte JL, Barbosa LT (2009) Artificial oxygen carriers as a possible alternative to red cells in clinical practice 12. Kulikovsky M, Gil T, Mettanes I, Karmeli R, Har-Shai Y (2009) Hyperbaric Oxygen Therapy for Non-Heating Wounds 13. Yoshida S, Kitano M, Eguchi H (1996) Water uptake and growth of cucumber plants (Cucumis sativus L.) under control of dissolved O2 concentration in 14. Owerkowicz T, Elsey RM, Hicks JW (2009) Atmospheric oxygen level affects growth trajectory, cardiopulmonary allometry and metabolic rate in the 15. Park JS, Kurata K (2009) Application of Microbubbles to Hydroponics Solution Promotes Lettuce Growth 16. Numako C, Nakai I (1995) Xafs Studies of Some Precipitation and Coloration Reaction Used in Analytical-Chemistry 17. Zhao G, He LQ, Zhang HF, Ding WP, Liu Z, et al. (2004) Trapped water of human erythrocytes and its application in cryopreservation 18. Barak M, Katz Y (2005) Microbubbles: pathophysiology and clinical implications 19. Cavalli R, Bisazza A, Giustetto P, Civra A, Lembo D, et al. (2009) Preparation and characterization of dextran nanobubbles for oxygen delivery