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1st micro lab report

By hayleyhue95 Dec 09, 2013 358 Words
Date of Experiment: September 3, 2013Date of Report: September 5, 2013 TITLE: Common Aseptic Transfers and Inoculation Method
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Aim: To transfer living microbes from one place to another aseptically (without contamination of oneself, others, the culture, or surroundings). Hypothesis: If microbes are said to be pure culture, after transferring them to a sterile medium aseptically, the microorganisms will grow in the nutrient broth plate and nutrient agar slant. Procedure: I first labeled the sterile broth tubes and nutrient agar plate with name, date, and organism I was inoculating with. Then I lit the Bunsen burner flame to sterilize the loop until the wire reached an orange hot point. I held the test tubes, agar plate and Escherichia coli tube at an angle to minimize airborne contamination, and swirled the sample of Escherichia coli. My partners’ samples were S. Aureus, B. Subtilis, and M. Luteus. I transferred the microbe to the sterile tubes and flamed the tubes lip two or three times each. Lastly I sterilized the loop and incubated the inoculated culture upside down at the assigned temperature for the assigned time. Results: E.coli had a great growth of yellow cloudiness with a few black specs in both tubes. The agar plate showed great growth as well with thick yellow lines of bacteria. My partner had S. aureus and that showed a darker mustard color on the plate. Her tubes were cloudy with one of them developed brown spots at the bottom. The third bacterium was B.subtilis which had more growth than any other ones and this one had a grayish color to it. B. Subtilis and M. Luteus had slight growth in both tubes; they had no big effect like the first two. Discussion and Conclusion: My results indicate that if transferring microbes without air bourne contamination, the microorganisms will grow in the agar plate and tubes at the given temperature. A couple mistakes that my group had was not getting enough bacteria in the tubes. The two bacteria’s that showed little growth were B. Subtilis and M. Luteus in normal body temperature. GRADING:

The format: 2 points
Each section of the body: 2 points each
Total: 12 points.

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