top-rated free essay

Spinach Lab

By ohahaha May 05, 2013 278 Words
In this lab, we extracted spinach pigments, and analyzed what colors of light these pigments absorb. By using TLC plate, hexane and acetone, I separated the pigments of spinach, and discovered that the main pigments were green and yellow. This works because with different polarities, pigments move at different rates. Hexane and acetone were also used to separate chlorophyll and carotene from spinach. Since they are polar, they can separate organic and inorganic things. From the experiment, I know that the pigments of spinach are mainly chlorophyll (green) and carotene (yellow). At first, I thought that they absorb the color which they present to us (the color we see of them). For example, I thought chlorophyll absorbs green light because it is green. However, I made a stupid mistake that the color they present to us is the color they reflect. Rather than absorbing green light, chlorophyll actually reflects it, and absorbs violet and red. This is because violet and red are two colors which are far away from green on a color spectrum. Since chlorophyll reflects green, it should absorb violet and red. The same thing applies to Carotene. It reflects yellow and absorbs blue light. One error we might have made is that we added a lot of hexane and acetone to our chlorophyll and carotene solutions. We did not have enough of them because they were exposed to light for several days. Although the final results are similar to the correct graphs, it is very likely that this has made some errors because the peaks are not at the exactly same wavelengths. By doing this lab continuously and quickly, this error can be prevented.

Cite This Document

Related Documents

  • Malunggay and Spinach Powder

    ...“MALUNGGAY (Moringa oelifera) AND SPINACH (Spinacia oleracea) POWDER AS FOOD SUPPLEMENTS” RATIONALE He gives us that vast goodness of nature and vegetation is among those. As man propagates for abundance; he deserves to harbour tremendous benefits from it. Man inhabited earth with all its richness laid before him. With his...

    Read More
  • malunggay and spinach powder

    ...³Malunggay andSpinach Powder´ ACKNOWLEDGMENTS The study aimed to introduce the combination of Malunggay and Spinach powder inorder to make a natural, nutritious and practical supplement for all especially to thosehealth-conscious individuals.Nevertheless, this study wouldnt be possible without the everlasting love, care andassistance from ...

    Read More
  • Computer Lab Rules & Regulations

    ...Computer Lab Rules & Regulations Electronic workstations may only be used by current Swinburne University students and staffs. Swinburne identity card must be presented on request. Swinburne computing facilities should only be used for educational, research and administrative purposes of Swinburne. All other uses are strictly prohibited. The...

    Read More
  • Computer Lab Managemetn System

    ...customer and stakeholder satisfaction, protect the brand and reputation, achieve continual improvement, promote innovation, remove barriers to trade, bring clarity to the marketplace. By using a proven management system, this will able to continually renew mission, strategies, operations and service levels. (

    Read More
  • Online Computer Labs Monitoring System


    Read More
  • Campus Computer Labs-the Issue at Hand

    ...Campus Computer Labs: The Issue At Hand As a student at Prince Georges Community College I like to take full advantage of all the resources available to me since at the end of the day I am paying for them. This sometimes includes staying a few hours on campus and doing my work instead of waiting until I get home to do it. One major problem th...

    Read More
  • Lab on a Chip

    ...3.2 “Lab-on-a-Chip” A Lab-on-a-Chip (LOC) device, also known as a micro-total-analytical system (microTAS) or microfluidics device, is a device that can integrate miniaturized laboratory functions (such as separation and analysis of components of a mixture) on a single microprocessor chip using extremely small fluid volumes on the orde...

    Read More
  • Case Analysis Kaspersky Lab: from Russia with Anti-Virus

    ...Case Analysis Kaspersky Lab: From Russia with Anti-virus Industry Background: Software Security Cybercrime has become a fast growing concern for the 21st century as businesses, institutions and individuals grow into an interconnected web of computer networks. Online business transactions, along with the sharing of personal information, ar...

    Read More

Discover the Best Free Essays on StudyMode

Conquer writer's block once and for all.

High Quality Essays

Our library contains thousands of carefully selected free research papers and essays.

Popular Topics

No matter the topic you're researching, chances are we have it covered.