Microbiology Lab Midterm Study Guide

Topics: Bacteria, Staining, Fungus Pages: 8 (2468 words) Published: October 29, 2014

Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1)
Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave, sharps containers, lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing, double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate building with separate ventilation and waste management systems Bacteria used in lab is classified as Class 1 by US Public Health Service/Biosafety Level 2 Review everything about the microscope and our original laboratory EXERCISE 1: USE AND CARE OF MICROSCOPE: (Covered 9.4.14 II Week 1) THE MICROSCOPE:

Simple microscope early microscope; like a magnifying glass
Brightfield compound microscope Most often used
Base Bottom of microscope
Stage Holds slide
Arm Carries the microscope
Body Tube Transmits the magnified image
Condenser Lenses that concentrate light by focusing it into a cone Iris Diaphragm Controls the angle and size of light cone
Objective lenses Magnify specimen
Ocular/eyepiece lens: 10x
Monocular One lens
Binocular Two lens
FOCUSING THE MICROSCOPE:
Coarse adjustment Focuses low power objectives (4x and 10x)
Fine adjustment Focusing with high-power (40x and 100x)
Field of vision Area seen through microscope
Magnification Depends on lens being used
Oil immersion lens 100x
RESOLUTION:
Resolving power/resolution Ability of lenses to reveal fine detail or two points distinctly separated Numerical aperture The lens system
Refractive index The amount the light bends
Focal point Where an image is formed
Spherical aberration Fuzzy periphery due to curvature of lens, resulting in multiple focal points Chromatic aberration Multitude of colors due to different wavelengths of light passing through lens Monochromatic light Lightsource with one wavelength of light eliminates aberrations Parfocal When a subject is in focus with all lenses

3) Understand the hanging drop and temporary wet mount and slides presented for observation EXERCISE 2: EXAMINATION OF LIVING MICROORGANISMS: (covered 9.11.14 II Week 2) Wet Mount and Hanging Drop were performed

PERFORM A WET MOUNT
Use pipette to transfer drop of sample to a slide
Cover sample with cover slip
PERFORM A HANGING DROP
Cover edges of coverslip with petroleum jelly
Place cover slip face-up on white paper towel
Transfer small drop of sample on coverslip
Plate depression slide over drop
VOCABULARY:
Brightfield microscopy Objects are dark and the field is bright Phase-contrast microscopy A ring of light is passed through the object and light rays are diffracted Diffracted Pushed aside
Brownian movement Not true motility but movement caused by molecules in liquid striking an object & causing it to shake or bounce What type of organisms could we expect to see in this lab?
Mainly protozoans. Some examples:
ALGAE PROTOZOA
Euglena
Diatoms
Chlamydomonas
Spirogyra
Volvox
Scenedesmus Paramescium
Amoeba
Stylonychia
Heteronema
Vorticella
Which type of compound microscope do we use in lab?
EXERCISE 3: MICROBES IN THE ENVIRONMENT (9.18.14 II Week 3)
BACTERIAL MORPHOLOGY: (Page 5 of Supplemental Guide)
MORPHOLOGICAL DESCRIPTION ASSOCIATED GENUS
Cocci that divide in one plane, forming a chain of cocci Streptococcus Cocci that divide in three planes at right angles, forming cubical packets Sarcina Cocci that divide in two planes, forming tetrads Gaffyka

Cocci that divide in two planes, forming irregular clusters 1. Staphyloccus 2. Micrococcus
DESCRIPTION BACTERIAL FORM
Bacilli with tapered ends, like a cigar Fusiform bacilli
Bacilli that resemble cocci Coccobacilli
Curved rods resembling commas, form S-shaped strands Vibrios Spiral-like corkscrews; rigid spirilla
Flexible spirals, with contractile axial filaments Spirochetes As...
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