MCB300 Exam 2 1. Which of the following statements is false? a. ATP synthase is found in all living organisms b. ATP synthase is a redox-driven proton pump c. ATP synthase can use proton motive force (PMF) to drive ATP synthesis d. ATP synthase can hydrolyze ATP to generate proton motive force (PMF) e. The Boyer model describes how rotary motion is used to synthesize ATP in ATP synthase 2. Which of the following is not associated with photosynthesis? a. Use of bacteriorhodopsin as a light-driven proton pump b. Light-driven oxidation of hydrogen sulfide in purple bacteria c. Oxygen production by Prochlorococcus d. Photoautotrophy e. All of the above are associated with photosynthesis 3. Correctly complete the following sentence: Cyclic photophosphorylation…. a. Is catalyzed by photosystem II in oxygenic phototrophs b. Is required to generate electrons for CO2 reduction in green sulfur bacteria c. Does not involve redox reactions d. Is a major source of ATP synthesis in obligately aerobic anoxygenic phototrophs e. Both “b” and “d” correctly complete the sentence 4. Correctly complete the following sentence: Syntrophic bacteria…. a. are required for conversion of biomass to methane and CO2 in methanogenic ecosystems. b. use protons as terminal electron acceptors. c. cannot grow to high density in the absence of methanogens. d. are involved in the sulfate-dependent anaerobic oxidation of methane. e. All of the above correctly complete the sentence 5. Which of the following mechanisms are not used by chemolithotrophs to bring electron donors and acceptors into close proximity? a. Symbiotic associations with eukaryotes. b. Storage of electron acceptors in large vacuoles. c. Movement between zones with high electron donor concentrations and zones with high electron acceptor concentrations. d. Cyclic photophosphorylation e. Both “a” and “d” are true 6. Correctly complete the following sentence: Metal respiration…. a. is often coupled to oxidation of glucose. b. sometimes uses tiny protein “nanowires” to transfer electrons to minerals. c. occurs only under acidic pH because Fe+3 is insoluble at neutral pH.
d. can be used to generate electricity in microbial fuel cells. e. Both “b” and “d” correctly complete the sentence 7. Which of following discoveries did not occur within the last thirty years? a. Proteorhodopsin-containing bacteria b. The existence of Prochlorococcus, the most abundant photosynthetic organism on earth c. The major role of obligately aerobic anoxygenic phototrophs in marine ecosystems d. The ABE fermentation e. Ammonia oxidizing archaea 8. Which of the following statements is false: a. Cyanobacteria are the only microbes to perform oxygenic photosynthesis. b. Cyanobacteria are responsible for approximately half of global photosynthesis c. Cyanobacteria are among the most abundant organisms on the planet. d. Cyanobacteria are the ancestors of chloroplasts in plants. e. All of the statements are true. 9. Based on the thermodynamics of the reaction, which chemolithotroph would be expected to have the highest growth yield using oxygen as the terminal electron acceptor? a. A nitrifying bacterium (Eo = +0.43 V for NO3-/NO2-) b. A nitrosifying bacterium (Eo = +0.34 V for NO2-/NH3) c. An iron oxidizing acidophile (Eo = +0.77 V for Fe+3/Fe+2) d. An iron oxidizing neutrophile (Eo = +0.2 V for Fe(OH)3 + HCO3-/FeCO3) e. A phosphite oxidizing bacterium (Eo = -0.69 V for HPO4-2/HPO3-2) 10. Which of the following is not energized by ion motive force in microbes? a. Rotation of the flagellum b. ATP synthesis c. Uptake of nutrients d. Substrate level phosphorylation e. Maintenance of cytoplasmic salt concentrations 11. Which chemolithotrophs would be expected to use reverse electron transport for CO2 fixation using the Calvin cycle (hint: requires NADPH; Eo = -0.32 V for NADP/NADPH)? a. A nitrifying bacterium (Eo = +0.43 V for NO3-/NO2-) b. A nitrosifying bacterium (Eo = +0.34 V for NO2-/NH3) c. An hydrogen oxizing acidophile...
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