Lab report: blood glucose regulation

LABORATORY REPORT

Activity 5: Blood Glucose Regulation
Name:
Instructor:
Date:

MATERIALS AND METHODS

1. Dependent Variable
Plasma levels of glucose, ketones, insulin, and glucagon

2. Independent Variable
Food and beverage intake

3. Controlled Variable physical activity, caffeine and alcohol intake, gender, age BMI

4. Why were physical activity and caffeine and alcohol intake the controlled variables?

Because you can control on the amount of time you spend doing physical activities and the intake of both caffeine and alcohol.

5. What type of assay was used to measure plasma glucose and ketone levels? glucose, oxidase, peroxide, dianisidine

6. What type of assay was used to measure plasma insulin and glucagon levels? glucose, oxidase, peroxide, dianisidine

RESULTS

See Table 2: Glucose and Ketone Concentration in Blood Plasma
See Graph: Glucose Concentration in Blood Plasma
See Graph: Ketone Concentration in Blood Plasma

Type in the following AVERAGE plasma glucose values:
Fasting:
Immediately post meal:
1 hour post meal:
2 hour post meal:
3 hour post meal:

Glucose (mmol/I)

Sub 1
Sub 2
Sub 3
Average
Fasting

Immediately Post Meal

1 Hour Post Meal

2 Hours Post Meal

3 Hours Post Meal

1. When was plasma glucose concentration highest? If values are similar for several time points (within a few points of each other), then give range of times when plasma glucose concentration was highest.

Immediately post meal and one hour post meal

2. When was plasma glucose concentration lowest? If values are similar for several time points, then give range of times when plasma glucose concentration was lowest.
3 hour post meal

Type in the following AVERAGE plasma ketone values:
Fasting:
Immediately post meal:
1 hour post meal:
2 hour post meal:
3 hour post meal:

Ketones (µmol/I)

Sub 1
Sub 2
Sub 3
Average
Fasting