Histology Flashcards

Topics: Lymphatic system, Epithelium, Lymph node Pages: 13 (3978 words) Published: August 26, 2013
SH

Lymphatic structures and organs

| Slide: Blood, humanStain/colour: H&ELink: http://vslide.med.unsw.edu.au/flashslide.jsp?fn=aacr_b33.svs&mag=80 Prominent features: * Identify the different kinds of blood cells. * Note the large number of RBCs compared to WBCs –doughnut shaped, no nuclei. Use as “measuring stick” – 7 microns. * Platelets are much smaller – purple fragments. * Monocytes: large, bean-shaped nuclei * Neutrophils: small granules in cytoplasm, multi-lobed. * Lymphocytes: large, evenly stained nucleus with little cytoplasm. * Natural killer cell: large “clockface” nucleus. Some granules in cytoplasm. * Eosinophil: bilobed nucleus, reddish granules (not stained well in this slide). * Basophils: rare and probably not visible.|

| Slide: Bone marrow smear, humanStain/colour: H&ELink: http://vslide.med.unsw.edu.au/flashslide.jsp?fn=b34_100xr.svs&mag=80 Prominent features: * Progenitor cells in various stages of development (do not have to identify specifically). These are not found in circulation. * Large white spaces – adiposecytes. * Note that structure has been destroyed by smearing process. * RBCs becoming paler as they mature – losing nuclei.Contains: * Megakaryocytes – large, dark progenitors breaking up into platelets. * Lymphocyte progenitors * Normoblasts – RBC progenitor. Band cells – WBC progenitor. * Haemopoietic stem cells give rise to all BCs.|

| Slide: Thymus, humanStain/colour: H&ELink: http://vslide.med.unsw.edu.au/flashslide.jsp?fn=l37_40xr.svs&mag=20 Prominent features: * Section of a single lobe of a thymus. * Contains lobules separated by CT septa. * Thin CT capsule (space is an artefact). * Septa do not extend all the way through. Contains BVs – thymic-blood barrier isolates maturing T cells from antigens in the blood. * Paler medullary regions surrounded by darker cortices, w. maturing (not proliferating) T cells. * Hassall’s corpuscles – found only in thymus. Made of reticulin.Contains: * Capsule: collagen, fibroblasts. * Stromal support cells, reticular cells, macrophages (paler staining regions).|

| Slide: Thymus, adultStain/colour: H&ELink: http://vslide.med.unsw.edu.au/flashslide.jsp?fn=adult_thymus_91_40xr.svs&mag=20 Prominent features: * The thymus has undergone involution as it has aged – functional tissue shrunken and replaced with adiposecytes. * Basic structure is still the same, e.g. blood vessels lie in stroma, medullary and cortical regions still present. * Capsule still in the upper right hand corner.Contains: * Capsule: collagen, fibroblasts. * Stromal support cells, reticular cells and fibres, macrophages (paler staining regions). * Maturing T lymphocytes. * Hassall’s corpuscles.|

| Slide: Thymus, humanStain/colour: H&ELink: http://vslide.med.unsw.edu.au/flashslide.jsp?fn=sc_15.svs&mag=20 Prominent features: * Section of a single lobe of a thymus. * Contains lobules separated by CT septa. * Thin CT capsule * Septa do not extend all the way through. Contains BVs – thymic-blood barrier isolates maturing T cells from antigens in the blood. * Paler medullary regions surrounded by darker cortices, w. maturing (not proliferating) T cells. * Hassall’s corpuscles – found only in thymus. Made of reticulin.Contains: * Capsule: collagen, fibroblasts. * Stromal support cells, reticular cells, macrophages (paler staining regions).|

| Slide: Spleen, normalStain/colour: H&ELink: http://vslide.med.unsw.edu.au/flashslide.jsp?fn=l36_40xr.svs&mag=20 Prominent features: * Thick CT capsule. Extends to form septa. * Paler-staining germinal centres, surrounded by dark cortex – white pulp regions. Small arterioles in the centre of these to allow antigens to enter. Surrounded by PALS (T cells) then B cells. Marginal zone contains lymphocytes and macrophages. * Red pulp regions – columns of cells form the cords of Billroth,...
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