Biomedical Engineer
Conclusion 1. Explain why antibodies allow scientists to target and identify specific disease agents.
Specific antibodies are linked to specific antigens. Scientists can track whether or not a person has a disease through the antibodies present in the body.
2. Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate?
So the substrate would turn a detectable form. It catalyzes so the antibody will be easily detachable.
3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100?
The one with 1/5 has a more detectable solution and tells scientists that they were one of the first to get it. The one with 1/100 has probably gotten it more recently. Because it is detectable at 1/100 the overall disease is most likely a strong form.
4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate.
A person could have a more conclusive result if they have 3 samples. Each time an ELISA assay is performed it gives a better look as to whether the person was positive or negative for the agent and the seriousness of the condition.
1. Why do you think college students living in dorms are often populations who see meningitis outbreaks?
Because at colleges, especially dormitories, students are cramped together and things like meningitis are easily spread. The students share common areas like bathrooms, drinking fountains, and guard rails of stairs.
5. How did ELISA data allow you to track the path of infection at the college?
It allowed us to track and find out the dilution of the disease in the patients. It also let us figure out who had the disease and who might have
Specific antibodies are linked to specific antigens. Scientists can track whether or not a person has a disease through the antibodies present in the body.
2. Why is the secondary antibody used in an ELISA test conjugated with an enzyme? What happens when this enzyme meets up with its substrate?
So the substrate would turn a detectable form. It catalyzes so the antibody will be easily detachable.
3. Disease samples from two patients are collected and subjected to serial dilutions before running an ELISA. What does it mean if a disease can be detected in samples from one person at a dilution of 1/5 and in another patient at a dilution of 1/100?
The one with 1/5 has a more detectable solution and tells scientists that they were one of the first to get it. The one with 1/100 has probably gotten it more recently. Because it is detectable at 1/100 the overall disease is most likely a strong form.
4. Describe a situation that illustrates why it is a good idea to complete the ELISA assay in triplicate.
A person could have a more conclusive result if they have 3 samples. Each time an ELISA assay is performed it gives a better look as to whether the person was positive or negative for the agent and the seriousness of the condition.
1. Why do you think college students living in dorms are often populations who see meningitis outbreaks?
Because at colleges, especially dormitories, students are cramped together and things like meningitis are easily spread. The students share common areas like bathrooms, drinking fountains, and guard rails of stairs.
5. How did ELISA data allow you to track the path of infection at the college?
It allowed us to track and find out the dilution of the disease in the patients. It also let us figure out who had the disease and who might have