5cm³ of distilled water‚ one in each of the water baths to bring them to the required temperature. Place a beetroot section in each boiling tube and leave for 30 minutes. Set up the colorimeter for 490 nm light absorption‚ and 2/3rds fill cuvettes with water from each of the 8 boiling tubes. Calibrate the colorimeter and put in the cuvettes‚ taking care to place them so the light passes through the smooth sides‚ and record the readings. Repeate the readings for all 8 temperatures to check results
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absorption occurs in the "green" region of the spectrum in both graphs 1 and 2. Graph 2 shows two peaks‚ one in the violet region (chlorophyll a) and one in the yellow-orange region at around the wavelength 670 nm. Due to the limitations of the colorimeter and its incapability of recording light absorbed by chlorophyll in between wavelengths of 600-700 nm‚ we did not have a recording for this region and thus no peak is shown. Also‚ in graph 1 ‚ there is more absoption of yellow light while in graph
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Investigating the effects of temperature on cell membranes Independent variable: Temperature of beetroot Dependent variable: Absorbency of light Hypothesis: As temperature rises to its optimum temperature‚ the absorbency of light will increase because protein is an enzyme and will therefore be affected by temperature. This is because more beetroot dye will pass through the cell membrane and then into the distilled water. The deeper the colour of the beetroot dye‚ the higher the absorbency
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decreased‚ and therefore the amount of pigment released from the beetroot samples is increased. The results produce a graph with negative correlation‚ a curve that has a negative gradient. The graph shows that as the temperature is increased‚ the colorimeter reading is decreased. The structure of my results proves my theory correct. The increase in
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absorbance. As the reaction between the chemicals takes place and the Crystal Violet receives the hydroxide the overall intensity of the purple color will decrease thus affecting the absorbance. The absorbance of the solution will be measured with a colorimeter as the reaction takes place and will be interpreted as a direct representation of concentration of Crystal Violet. After the reaction has taken place‚ through analysis of graphs plotting absorption vs. time‚ the natural log of absorption vs. time
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Apparatus Material and Equipment: 0.00200 M KNCS‚ 0.00200 M Fe(NO3)3 0.200 M Fe(NO3)3‚ 0.10 M HNO3‚ 5 Cuvettes‚ 1 colorimeter 4 100ml beakers‚ 5 Test tubes 2 250ml Graduated cylinder. The first step is to calibrate the colorimeter with0.20 M Fe(NO3)3and set the absorbance at 470 nm since it is known to keep an acidic solution throughout the entirety of the experiment. It was important to do this right at the beginning of the lab since the zeroed value of the acid was the
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An investigation to measure the percentage light transmission‚ using a colorimeter‚ through a solution‚ from pH 2 – pH 9‚ in which jelly cubes were immersed over a 24 hour period Interpretation Written Communication of the Data C1 Pepsin is an enzyme that works in the stomach and has an optimal pH between pH 1 and 4 or in acidic conditions. From our graph it can be seen that that the lowest mean percentage light transmission for pepsin is when the buffer has a pH of 2. Trypsin is an enzyme that
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absorbance of some light wavelengths by solutes dissolved in solution‚ while allowing other wavelengths to pass through (transmittance). The combination of the remaining wavelengths that pass through results in the colour of the solution. A colorimeter can be used to determine the amount of light at a particular wavelength that is absorbed/transmitted by a solution. Depending on the concentration of the solute in the solution‚ more or less absorbance/transmittance will result. A = lc where
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Biology Lab Report - Movement in and out of cells (4A) Shiang Chen Ting (24) Sec 313 Introduction The aim of this experiment is to explore the factors which affect the movement of materials in and out of the cells. The dark red or purple colour of Beetroots is caused by a pigment called betalain that is found within the vacuole of the beetroot cells. The pigment remains intact within the cell if the cells are not “stressed” by the external environment. In this exploration‚ two factors that affect
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Unit Name: Analysis at Work Unit Number: G621 Assignment Title: Finding the Percentage of Copper in a Brass Alloy Assignment: G620 Sample Assignment C Date Set: Due Date: Assessment Objective(s): AO3 Vocational Brief: Brass is an alloy of copper and zinc. This alloy has many uses ranging from coinage to non-ferrous fittings on ships. Many domestic taps use brass where contact with water occurs. You are provided with a sample of brass which is about 150 years old and you are
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