The main proteins for MPTP function and regulation are ANT and Cyp D. Cyp D binds to the ANT and sensitized the MPTP to calcium and facilitates MPTP opening, suggested that Pi has the same function, also Pi control the matrix pH when generation polyphosphates groups or decreasing magnesium of matrix [113].
During ischemia reperfusion, surprisingly at the, MPTP is opened and Pi is elevated. There is calcium overloading thanks to ROS generation, thus necrosis and apoptosis happen