Evaluation of Paracetamol Content in Dosage Form Using UV - Visible Spectrophotometry
Evaluation of Paracetamol Content in Tablet Dosage Form
Using UV-Spectrophotometry
Casilagan, Camela Mae C., and Jimenez, Joshua M.
Chemistry Department, College of Science
Adamson University
Ermita, Manila
1.0 Introduction
UV-Visible Spectrophotometry is a widely-used technique in Chemical and Pharmaceutical Analysis. And this method has been very useful both for Qualitative and Quantitative Applications, especially for organic compounds. Some of its advantages for Quantitative purposes arise from its wide applicability for analysis of compounds, and its apparent speed of performing analyses, with the use of modern technology applied to these instruments (Skoog, et.al, 2014).
Paracetamol,(N-(4-hydroxyphenyl) acetamide) is classified as to being part of the drugs known as “aniline analgesics” (Behera,S.,et.al, 2012) and is widely used as analgesic and antipyretic drug, together with caffeine, ibuprofen, and diclofenac sodium. Based on the United States Pharmacopeial Convention, Titrimetric, Thin Layer Chromatographic Identification Test, as well as UV spectrophotometric assay determinations have been suggested as determination methods for Paracetamol in tablet formations.
For Paracetamol, various methods have been made known in its determination in bulk form, which is based on its reaction with Iron (III) and Ferricyanide in an HCl medium (Nagedra, P., 2010), or through a simultaneous determination of Paracetamol with another component, like Etodolac (Balan, P., et.al, 2011), Thiocolchicoside and Aceclofenac (Hapse, S.A., et.al, 2011) and Tramadol HCl ( Sawant, R.,et.al., 2011).
Figure : Paracetamol.
This experiment focused on the determination of the Paracetamol content of Biogesic®, and to assess the Percentage Label Claim of the branded Paracetamol Tablet, in comparison to a USP Grade Paracetamol Standard.
2.0 Methodology
Use the number format in separating procedures. Use past-passive tense.
2.1 Materials and Reagents
Using UV-Spectrophotometry
Casilagan, Camela Mae C., and Jimenez, Joshua M.
Chemistry Department, College of Science
Adamson University
Ermita, Manila
1.0 Introduction
UV-Visible Spectrophotometry is a widely-used technique in Chemical and Pharmaceutical Analysis. And this method has been very useful both for Qualitative and Quantitative Applications, especially for organic compounds. Some of its advantages for Quantitative purposes arise from its wide applicability for analysis of compounds, and its apparent speed of performing analyses, with the use of modern technology applied to these instruments (Skoog, et.al, 2014).
Paracetamol,(N-(4-hydroxyphenyl) acetamide) is classified as to being part of the drugs known as “aniline analgesics” (Behera,S.,et.al, 2012) and is widely used as analgesic and antipyretic drug, together with caffeine, ibuprofen, and diclofenac sodium. Based on the United States Pharmacopeial Convention, Titrimetric, Thin Layer Chromatographic Identification Test, as well as UV spectrophotometric assay determinations have been suggested as determination methods for Paracetamol in tablet formations.
For Paracetamol, various methods have been made known in its determination in bulk form, which is based on its reaction with Iron (III) and Ferricyanide in an HCl medium (Nagedra, P., 2010), or through a simultaneous determination of Paracetamol with another component, like Etodolac (Balan, P., et.al, 2011), Thiocolchicoside and Aceclofenac (Hapse, S.A., et.al, 2011) and Tramadol HCl ( Sawant, R.,et.al., 2011).
Figure : Paracetamol.
This experiment focused on the determination of the Paracetamol content of Biogesic®, and to assess the Percentage Label Claim of the branded Paracetamol Tablet, in comparison to a USP Grade Paracetamol Standard.
2.0 Methodology
Use the number format in separating procedures. Use past-passive tense.
2.1 Materials and Reagents
References: Balan., P., et.al (2011) Simultaneous Estimation of Etodolac and Paracetamol by UV Spectrophotometric method in tablet formulation. Journal of Pharmacy Research, 4(6) 1663-1665. Retrieved from http://jpronline.info Behera et.al (2012) UV-Visible Spectrophotometric Method and Validation of Assay of Paracetamol Tablet Formation. Journal of Analytical & Bioanalytical Techniques. 3(6), http://dx.doi.org/10.4172/2155-9872.1000151 Nagedra,P. (2010) Spectrophotometric Estimation of Paracetamol in Bulk and Pharmaceutical Formulations. E-Journal of Chemistry. Volume 8(1), 149-152. Retrieved from http://e-jounals.net. Sawant., R. et.al. (2010) Validated Spectrophotometric Methods For Simultaneous Estimation of Paracetamol, Domperidone, and Tramadol HCl in pure and tablet dosage form. Journal of Chemical Metrology 4(1), 21-27. Skoog., et.al (2014) Skoog and West’s Fundamentals of Analytical Chemistry, Ninth Edition , International Edition. Brooks/Cole Publishing. United States Pharmacopeial Convention (2011), The United States Pharmacopeia, the National Formulary (USP 35 NF 30) Volume 2., 2032-2035 The United States Pharmacopeial Convention, 12601 Twinbrook Parkway, Rockville MD 20852