Lab Experiment number 11
To learn the Gram stain technique, the reason for the stain, and how to identify the results of the organisms stained.
Bunsen burner, inoculating loop, staining tray, glass slides, bibulous paper, lens paper, oil, and microscope
Differential StainingGramStaining and Acid Fast Staining
Differential Staining, one which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between...
Theories of Staining Techniques
Staining bacteria with different dyes via staining techniques, allows in distinguishing the microorganism from its backgrounds. Also, helps in studying different internal structures such as vacuoles, cell walls and spores in details (Seeley and others 1991). Some staining...
Isolation of Pure Cultures by Dilution Techniques and GramStaining Method
Table 1. Gram stain reaction and cellular features of the culture.
Gramstaining methods were applied on the given mixture of Bacillus cereus,
Escherichia coli and Staphylococcus aureus and then examined microscopically...
bacteriology, stain is probably the most important and useful method to differentiate types of bacteria. There are three different staining processes will be performed, which are gramstaining, acid-fast stain and spore stain
During the gramstaining, bacteria are divided into gram positive and negative or...
This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope...
in every step done in the process of isolation and identification.
Proper use of staining methods and culture media will make individual protocol easy to perform. For this crucial selection of procedure, avoid complication and discrepancy of result.
To review some important methods which include:...
nucleus containing genetic material
Unicellular (yeasts) or multicellular (mushrooms)
Cell wall of chitin
Asexual and sexual reproduction
Molds: the most typical fungus: form visible masses (mycelia)
Nutrition obtained by absorbing it from their environment (soil, water, plant or animal host)
TOUCH THE OIL.
Just use FINE ADJUSTMENT.
We used OIL immersion (100x) for the bacteria we observed. Why?
Bacteria are so small that we must use the most powerful objective.
Difference between magnification and resolution (#3)?
Magnification is enlarging the image.
Ocular is 10x
Objectives are 4,...
To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood.
PROCEDURES: Using the microscope, an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood:
well-managed agro-ecosystem.soil flora, particularly fungi by their enormous mycelial hyphae entangle the soil particles and improve soil aggregation, which in turn improves soil aeration, infiltration and root elongation. Rhizosphere, microorganisms particularly plant growth-promoting rhizobacteria (PGPR)...
EXPLAIN THE PRINCIPLE AND DESCRIBE THE PROCEDURE (GIVING RATIONALE FOR EACH STEP) OF: GRAM STAIN ANDACID-FAST STAIN
The gram stain is a differential stainingprocedure because it divides bacteria into two classes –gram negative and gram positive it identifies the nature of the cell and the test results...
GRAMSTAININGGramstaining depends on the thickness of the peptidoglycan layer of bacteria. Recall that typical Gram-positive bacteria have a multi-layered, thickened peptidoglycan layer, while Gram-positive bacteria have a thinner peptidoglycan layer sitting in the middle of the periplasmic space...
Microbiology July 8, 2011
Overview of Food Microbiology
OBJECTIVES At the end of this module, you will be able to: 1. Explain the structural similarities and/or differences among Gram-positive and Gram-negative bacteria as well as their isolation and identification using serological, biochemical, and molecular...
1.What is the purpose of staining samples?A) To increase the contrast and visibility of the specimen
B) To kill the specimen
C) To see the specimen without the aid of a microscope
D) To make the specimen appear larger in the microscope2.Which of the following are bacterial SHAPES? (Select all that...
differential tests used to identify the unknown cultures were Gram stain, Catalase, Mannitol Salt Agar (MSA), Blood Agar, Novobiocin, Coagulase, and DNAse (Alachi, 2007).
February 21, 2012
Staining and Identifying Unknown Bacteria