• Lab Report
    another on the basis of the stain that is retained. Source: Microbiology – A Laboratory Manual 4th Edition/ James G. Cappuccino, Natalie Sherman/ 2008/ Pages 73  Question 4: which is the most crucial step in the performance of the Gram staining procedures? Explain. Answer: Decolorization is the most...
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  • Fwr Bacteriology
    step in the identification of an unknown prokaryotic organism. For eukaryotic cells this stain is not significant, since most of them are Gram Negative, except yeasts and few molds which are Gram Positive. The bacterium Staphylococcus aureus is considered as gram positive (purple) since it resists...
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  • Theory of Staining Techniques
    endospore was found in our unknown culture. The endospores appeared ruptured and damaged. This could be due to an error in the procedural steps above as to the allotted time of staining is very crucial when performing this procedure. However, a normal observation of an endospore staining would have...
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  • Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method
    viewed during microscopic observation. During Gram staining, the most crucial step in determining the outcome is the decolourisation by alcohol step. If alcohol is applied too long, the alcohol may wash all the CV-I complex that is trapped in peptidoglycan layer. Therefore, alcohol is only applied...
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  • Biolab
    Introduction In bacteriology, stain is probably the most important and useful method to differentiate types of bacteria. There are three different staining processes will be performed, which are gram staining, acid-fast stain and spore stain . During the gram staining, bacteria are divided...
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  • Microbiology
    3. Which step is the most crucial or most likely to cause poor results in the Gram stain? Why? 4. Why must young cultures be used when doing a Gram stain? 5. Why was H. neptunium Gram stained? 6. What is meant by gram variable? 7. What part of the bacterial cell is most involved with...
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  • BST Week 1
    , that each step was performed aseptically.             Gram staining played a critical role in the identification of the microorganism. Gram staining is widely used to eliminate thousands of possible organisms because it confirms Gram negative or Gram positive organisms and cell morphology. In this...
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  • Lab Report about Simple Staining of Microbes
    organic washing procedure and those that do not. Gram Staining is the most consistent when done on bacteria that less than 24 hours, while the older cultures may not retain the primary stain and give inaccurate results. 2.0 Literature Review Instrument that is used to see objects that are too small...
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  • studyworld34
    every step done in the process of isolation and identification. Proper use of staining methods and culture media will make individual protocol easy to perform. For this crucial selection of procedure, avoid complication and discrepancy of result. To review some important methods which include: (1...
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  • The Microbiology of Biological Phosphorus Removal in Activated Sludge Systems
    sensitive receiving bodies of water, most still operate unreliably. Such unreliable performance persuaded the US Water Environment Research Foundation to fund projects R.J. Seviour et al. / FEMS Microbiology Reviews 27 (2003) 99^127 101 in 2001 to investigate technology yielding consistently...
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  • Microbiology
    stains – react differently with different kinds of bacteria Allow for distinguishing different bacteria Gram stain Acid-fast stain Gram Stain Most useful staining procedures Classifies bacteria into two large groups: Gram-positive and gram-negative Procedure : Heat fixed smear is...
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  • Microbiology Labs
    gram-negative and gram-positive bacteria. PROCEDURES: Preparation of Bacterial Cultures, Differential Staining. I prepared a nutrient agar dish as previously described and I prepared a S. epidermidis culture and an E. coli culture. I also used the MRS broth media and a sample cup to prepare...
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  • Svegf
    placed in microscope under oil immersion and observed. The Gram stain is almost always the first step in the identification of a bacterial organism. Gram staining uses crystal violet to stain cell walls, iodine as a mordant, and a fuchsin or safranin counterstain to mark...
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  • Lab Exam 1 Review
    /- ) – repelled by negative parts of cell and can only surround the cell Staining Simple = 1 stain used  examine shape/arrangement of bacteria Differential = 2+ stains used  differentiate between bacteria Examples: Gram staining, Acid-fast staining Positive = using a basic (+) dye  in this...
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  • Microbiology
    Food Microbiology July 8, 2011 Gram-positive bacteria (those that retain the purple crystal violet dye when subjected to the Gram-staining procedure) - the cell wall adjoining the inner or cytoplasmic membrane is thick (15-80 nanometers), consisting of several layers of peptidoglycan, also known as...
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  • micro bio
     EXPLAIN THE PRINCIPLE AND DESCRIBE THE PROCEDURE (GIVING RATIONALE FOR EACH STEP) OF: GRAM STAIN ANDACID-FAST STAIN The gram stain is a differential staining procedure because it divides bacteria into two classes –gram negative and gram positive it identifies the nature of the cell and the test...
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  • Evaluation of Effectivities of Soaps
    . Staining procedure GRAM +VE AND GRAM –VE CELL WALL Gram positive appear- blue to transfer to purple Structure: Have thick layer of peptidoglycan over inner cytoplasmic membrane. lack LPS-lipopolysaccharides Gram negative appear: pink to transfer to...
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  • Unknow Lab Report
    . There are several types of staining through which the bacteria can be identified based on the color and shape. The staining methods used in the lab are Gram Staining, Capsule Staining, Endospore Staining, and Acid Fast staining. One of the most significant method of staining is the Gram Staining...
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  • Microbiology 311 Lab Report
    can be identified based on the color and shape. The staining methods used in the lab are Gram Staining, Capsule Staining, Endospore Staining, and Acid Fast staining. One of the most significant method of staining is the Gram Staining, as it is highly dependent (McCarthy, 25). In the specific...
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  • essay
    Gram staining has become a useful and universal technique in the microbiology world founded in 1883 by a Danish bacterialist names Hans Christian Gram. It has become the most crucial first procedure performed to test for the identification of bacteria. The reason for the actual staining is to...
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