• Lab Set
    , which uses crystal violet plus additional stains, will show the structure of a cell wall and even how many membranes the bacteria has. 2. What are the differences between gram positive and gram negative cell walls? (2) The unique characteristic of Gram positive bacterium is the make-up of its...
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  • Gram Staining
    Micro Biology Lab Gram Staining Lab Report Purpose The purpose of this lab was to experiment with a staining technique called Gram Staining. Gram Stains is used to identify and classify bacteria, it is a differential stain that allows you to classify bacteria as either gram-positive or gram...
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  • Unknown Rport on Bacillus cereus
    Crystal violet stain in the cell and therefore the cell remains purple. Figure 3 an example of B. cereus that has been Gram stained Lab Day 4: To determine whether my unknown bacteria produced endospores I needed to perform an endospore stain. An actively growing and metabolizing bacterium is...
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  • Microbiology Practical
    bacteria is broad spectrum, the least is narrow spectrum. Staining Procedures- Gram Stain- 1. Inoculate bacteria- Smear it into a drop of water- Let it Dry- Heat Fix it 2. Apply Primary Stain (Crystal Violet)- Let sit 60 Seconds- Rinse 3. Apply Binding Agent(Mordant)- Let sit 60 Seconds- Rinse 4...
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  • Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method
    species into two groups. The Gram staining technique was developed by a Danish doctor, Hans Christian Gram in 1884 and modified by Hucker. Gram-positive bacterias are stained purple while Gram-negative bacterias are stained pink. In Gram staining, a purple crystal violet stain is used to stain the...
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  • Medical Unknown
    incubation ( Blood agar , DNase and MSA plates ) . The following table lists all of the tests were done : Test | Purpose | Reagents | Observations | Results | Gram stain | To determine The gram rxn ofBacterium. | Crystal violet,Iodine, Alcohol&Safranin. | Purple cocci | Gram...
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  • Biophysics Lab
    bacteria are first stained with the basic dye crystal violet. Both Gram-positive and Gramnegative bacteria become directly stained and appear purple after this step. 2. The bacteria are then treated with Gram’s iodine solution. This allows the stain to be retained better by forming an insoluble crystal...
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  • People
    away the crystal violet. The next step is to stain with safranin which will cause the smear to turn a pink color. If the bacteria is Gram negative the bacteria will appear pink under the microscope because the alcohol washed away the crystal violet and it uptook the safranin. If the bacteria is...
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  • Conceptual Approach
    makes staining difficult? 2. Specifically, what does it mean when an organism is termed “acid-fast”? How does the decolorizer in the acid-fast procedure differ from that used in the Gram stain procedure? Colony Morphology Along with the Gram stain, colony morphology is one of...
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  • Unknown Microbiology Lab Report
    streak at line of stab | Sulfide negative Indole negativeNon motile | Table 2: Biochemical Test Results (Bacteria # 2) TEST | PURPOSE | REAGENTS | OBSERVATIONS | RESULTS | Gram Stain | To determine the Gram reaction of Bacteria # 2 | Crystal violet, Iodine, Alcohol, Safranin | Pink rods...
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  • Unknown Lab Report
    . epidermidis – simple stain S. epidermidis – simple stain Following the simple stain, the Gram Stain was performed. The Gram Stain is a procedure used to determine the presence or lack of peptidoglycan in the cell walls of a bacterium. The process began with flaming the inoculation loop, and...
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  • Nothing
    .   Steps in the gram stain:          Crystal violet is applied to a heat-fixed smear for 30 seconds and gently rinsed off.  This is the primary stain.          The smear is thoroughly covered with Gram’s iodine, which is left for 1 minute and then rinsed.  The iodine acts as a mordant, fixing...
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  • lab of microbiology
    bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranine. Gram-positive bacteria retain the violet stain; gram-negative bacteria do not. That means gram-negative bacteria is decolorized...
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  • Unknown Paper
    Gram-negative bacteria and differentiate them for lactose fermentation. It contains bile salts (to inhibit most Gram-positive bacteria), crystal violet dye (which also inhibits certain Gram-positive bacteria), neutral red dye (which stains microbes fermenting lactose), lactose and peptone. Eosin...
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  • Mr Kunle Fadeyi
    under a microscope, and using a stain increases the contrast of a cell with its background. Staining a cell with a dye such as Giemsa stain or crystal violet allows a microscopist to describe its size, shape, internal and external components and its associations with other cells. The response of...
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  • Microbiology Study Guide
    bacteria make them attracted to the positive chromospheres of basic dye? Bacteria are slightly negative, therefore they’re attracted to the positive chromophore. 19. Name some common dyes. Grams, Crystal Violet, Basic Fuchsion, Safranin, Methylene Blue. 20. What is the role of the Mordant in...
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  • Svegf
    , to it 4 reagents are added for the purpose of staining , first a drop of crystal violet is added and washed within 1min,then to it grams iodine is added and washed for 1 min later it is washed with decoloursier alcohol and water, later saffronin is added and washed for 1 min and dried and then it is...
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  • Antimicrobial Activity of Soil- Research Proposal Abstract
    . * API test kit Biomerieux (2009) * MATERIALS TO BE USED GRAM STAIN | GENERAL MATERIALS | Lugols’ iodine | Laboratory protective coat | Safrannin | Examination gloves | Acetone | Biohazard disposal bags | Crystal violet | Sterile sample containers | | Garden hoe | CULTURE...
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  • Stydy Guide
    , resolution is more important than magnification. You have learnt many staining techniques through the lab exercises: What dye did you use in simple stain? Crystal Violet What dyes did you used in Gram stain? Crystal violet, gram’s iodine, and safranin violet. Why did you have to heat up the...
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  • Cornyebacterium
    Gram stain was used to differentiate the organism based on cell wall composition. The stain was prepared with crystal violet, followed by Gram’s iodine, 70% ethanol and safranin. These three different strains were used to identify the positive and negative cell walls by dying them pink if negative...
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