Labset Two Worksheet
1. What is differential staining? How does it differ from simple staining? (2)
Differential staining is a stain that is used to differentiate one area or cellular structure from another. It is also to differentiate a particular thing from another in a specimen. This is done...
Simple Stain- Uses 1 Stain
1. Acid Stains (- Charge)- Stains Background- Nigrosin, India Ink and Congo Red
2. Base Stains (+ Charge)- Stains Cell- Methylene Blue, CrystalViolet, Safranin
Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It...
Gram Staining Lab Report
The purpose of this lab was to experiment with a staining technique called Gram Staining. GramStains is used to identify and classify bacteria, it is a differential stain that allows you to classify bacteria as either gram-positive or gram-negative. The...
Enzyme: Provide a surface on which reactions take place
• Active site – area on enzyme surface where enzyme forms a loose association with substrate(s)
• Substrate – substance on which enzyme acts
• Properties of enzymes:In general, chemical reactions that release energy (exergonic reactions) do not...
Biology 302 Exam 2 Answer Sheet: must be submitted as a .doc or .docx file
|Student’s Name: |S |
Please place your answers in the following tables. Please use Capitol Letters. (1 point each)
1 |D |13 |D |25 |D |37...
Differential Gram’s staining
EXPERIMENT NO. 1 AIM: THEORY:
Page No: C1 Date:
To Gramstain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult, not just because they...
Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method
Table 1. Gramstain reaction and cellular features of the culture.
Gram staining methods were applied on the given mixture of Bacillus cereus,
Escherichia coli and Staphylococcus aureus and then examined microscopically...
LITERATURE REVIEW 3
AIM OF RESEARCH 4
OBJECTIVES OF STUDY 4
RESEARCH METHODOLOGY 4
METHOD(S) TO FACILITATE MICROBIAL IDENTIFICATION 5
MATERIALS TO BE USED 5
ETHICAL CONSIDERATIONS 6
SAMPLE COLLECTION 6
SAMPLE ANALYSIS METHOD...
extracts against S. aureus coagulase production.
Specifically the researcher aims to achieve the following:
1. To identify herbal plants that have inhibitory effect against S. aureus coagulase production
2. To quantify the inhibitory effect of herbal plants on coagulase production in S. aureus
the specific epithet, coli, reminds us that E. coli live in the colon, large intestine. Both S. aureus and E. coli will be studied extensively this semester.
Types of Microbes
A.Bacteria (pl.)/ Bacterium (s.)
unicellular prokaryote (prokaryote (pro=first; kary=nut, nucleus) – genetic material not enclosed...
UNKNOW BACTERIA LAB REPORT
The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This...
epidermidis”, S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis...
physiological characteristics will be determined by performing a list of biochemical tests that pertain to each of the organisms. In addition to discovering what my unknown is, I need to fully understand the chemistry of the tests. I will cultivate the organisms and perform the tests to discover my unknown....
allowed to air dry; stain is then applied and slide is observed under microscope
Simple stain – aqueous or alcohol solution of a single basic dye
Highlights the entire microorganism so that cellular shapes and basic structure can be observed
Differential stains – react differently...
opinion regarding Grams staining method and reliability of this method. According to me, Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though, Gram is concerned that...
bone abscess or a spongiform encephalopathy produced by a prion.
Four nutrient agar plates growing colonies of common Gram negative bacteria.
Microbiological culture is a principal tool used to diagnose infectious disease. In a microbial culture, a growth medium is provided...
Leeuwenhoek, Louis Pasteur, Robert Koch, Edward Jenner, Paul Ehrlich, Lister and Fleming.
* Robert Hooke was an English scientist in the early 1660’s. He was the first person to use a microscope for academic study and he was also the first observations.
* Antoine van Leeuwenhiek was introduced the...
Knowing of unknown microorganism is important on how this microorganism works and how it is structured, means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory...
aiding in the cleanup of polluted soils, as well as assist in the decomposition of human waste (Dhamani, 2001).
Since discovered in the late 1600’s by Anton Von Leeuwenhoek, bacteria in the environment have become a main focus in many studies (Black, 2012). It was once believed that bacteria only...