Methyl Red and Voges-Proskauer Test The Methyl Red test shows which bacteria are creating stable acids through mixed acid fermentation of glucose. This helps to identify enteric bacteria by examining how they metabolise glucose. Every enteric bacteria first produces pyruvic acid from metabolism of glucose. A methyl red positive enteric bacteria‚ uses the mixed acid pathway when breaking down pyruvic acid to different acids‚ like lactic‚ acetic‚ and formic acids. The Voges-Proskauer test determines
Free Bacteria Metabolism Protein
Methyl Red Voges-Proskauer Test Questions A.What is meant by the term biochemical profile? Biochemical profile is a variety of biochemical tests. These various tests are used to measure the constituents of plasma and serum in blood. B.What metabolic end product does the MR test for? The metabolic end product MR tests for are the different bacteria’s related to their individual patterns of glucose metabolism. C.What does an orange color indicate as a result for an MR test? An orange color as a result
Premium Metabolism Bacteria Oxygen
Staphylococcus epidermidis is one of the most prevalent microorganisms found on the human skin and in the mucous membranes‚ however‚ it is a typically overlooked bacterium because there is very little that is known about it. Though it is not as aggressive as its cousin Staphylococcus aureus‚ Staphylococcus epidermidis is the most frequent cause for nosocomial pathogens‚ especially among newborns‚ the elderly‚ and anyone who has a compromised immune system. These infections are usually associated
Premium Bacteria Immune system Staphylococcus
‚2011 SPECTROPHOTOMETRIC DETERMINATION OF THE ACID DISSOCIATION CONSTANT OF METHYL RED M.C. Caligagan ‚M.N.Q. Tolentino and M.Q. Clores Institute of Chemistry‚ College of Science University of the Philippines‚ Diliman‚ Quezon City‚ Philippines Received :September 21‚2011 ABSTRACT This experiment aims to determine the acid dissociation constant value of Methyl Red by means of spectrophotometry. Ten samples were spectrophotometrically analyzed under a UV-Vis spectrophotometer and from
Premium Acid dissociation constant
In this experiment‚ we used methyl benzoate from the last experiment with HNO3 and H2SO4 to synthesize methyl 3-nitrobenzoate. First we added methyl benzoate to 12 mL cooled conc. Sulfuric acid in a flask. In a separate flask‚ we made a solution of 4 mL conc. Sulfuric acid and 4 mL nitric acid and then added dropwise to the solution of methyl benzoate in an ice bath on a stir plate while maintaining the temperature of reaction between 5-15 °C. After the addition was complete we took the flask out
Premium Water Chemistry Acetic acid
para-hydroxybenzoic acid‚ which is why they are collectively called parabens. However‚ in contrast to its cousins‚ ethylparaben‚ butylparaben‚ and propylparaben‚ methylparaben receives its specific name owing to the fact that its chemical structure contains the methyl alkyl group. Methylparaben is found in several fruits‚ in particular blueberries‚ where it acts as an antimicrobial agent. Methylparaben is an anti-fungal agent often used in a variety of cosmetics and personal-care products. Methylparaben is commonly
Premium
This project’s purpose is to find the effects of UV light on Staphylococcus epidermidis‚ furthermore recognized as staph infection. Staph infection is an infection usually acquired on the skin that can promote fever‚ nausea‚ pain‚ boils‚ and can lead to MRSA‚ which can lead to death. Because there is no cure for staph infection‚ this experiment called for research on clinical trials. A clinical trial was established that used blue light therapy to delay and kill the growth of staph infection. Coincidentally
Premium Bacteria DNA Scientific method
The role of the governor-general The Governor-General represents the Australian head of state‚ Queen Elizabeth II. Between them they have important formal powers. Some of these powers include the power to: h approve legislation passed by the two Houses of Parliament; h command the navel and military forces; h Exercise the general ’executive’ power of the Commonwealth. Many of these powers reflect an earlier‚ more active role taken by the monarch. In almost all cases today‚ however‚
Premium Australia Separation of powers Elizabeth II of the United Kingdom
In this experiment the initial mass of the sodium carbonate used was 2.69g. In each titration‚ 3 drops of methyl orange was added to the sodium carbonate solution. With this information the titration can begin‚ and the results obtained are shown below: Titration readings Titration Rough 1 2 3 4 5 Initial 0.00 4.30 22.00 21.00 15.00 25.90 Final 4.30 22.00 38.60 37.60 32.60 42.20 Titre (cm3) 4.30 17.70 16.60 16.60 17.60 16.30 Therefore‚ the average titre would be calculated as follows;
Premium Titration Chemistry Mole
Nitration of Methyl Benzoate Introduction: Nitration is an example of an electrophile aromatic substitution reaction‚ where nitro (NO2) group is being substituted for a hydrogen on an aromatic compound. This is achieved by the formation of the nitronium ion by protonation of nitric acid from sulfuric acid. The zirconium ion is a strong electrophile and can react with aromatic compound such as Methyl benzoate to form an arenium ion intermediate. The arenium ion is then depronated to reform
Premium Benzene Electrophilic aromatic substitution Sulfuric acid