Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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which organism we had in our unknown mixed culture tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I
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Gabi Mejia Chem 101 Section ADF Lab 4: Weak Acid Unknown Procedure: When testing the acid‚ use only between 0.2 g and 0.3 g for each trial (get as precise a measurement as you can). The general procedure is to weigh out your acid‚ dissolve it in water‚ add a couple drops of the indicator (phenolphthalein)‚ and then add the sodium hydroxide until you note a color change (from clear to pink). When the color change occurs‚ you have added enough base to completely react with the acid (the endpoint).
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Jolyne Piet CHM-221L-02 Lab #2: Experimental Design Isolation of Sucrose: 3.01 g Panacetin were weighed in a 125-mL Erlenmeyer flask‚ and 51mL dichloromethane were added to partially dissolve the Panacetin. The insoluble portion was gravity filtered and air dried to yield 0.45 g of sucrose (15.0 % of original Panacetin). Isolation of Aspirin: The organic filtrate was extracted through a separatory funnel with 32 mL 5% sodium bicarbonate to produce an aqueous layer and a dichloromethane layer
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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Introduction The purpose of this lab was to explore the properties of an unknown compound. An unknown was given and a cation flame test and anion test was performed to determine the identity of the compound. Once the identity was determined‚ the properties were explored. Experimental To determine the cation of the compound‚ a cation flame test was performed. A bunsen burner was lit until a medium blue flame was burning. The given unknown was scooped onto a nichrome wire loop. The wire was held
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LAB#: 20 SKILL: Planning and Designing OBSERVATIONS: A student is given a small beaker containing an unknown salt‚ x. The salt is crystalline‚ deliquescent and colorless. The student is asked to perform test and observation on the salt to determine the cation and anion present. HYPOTHESIS: Perhaps by using the flame test or reacting salt x with NaOH‚ or NH4OH the cation could be distinguished by observing the color changes or solubility while reacting salt x with H2SO4 or a mixture of copper
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EXPERIMENT ONE: ‘’The Scientific Method’’ Lab Report by Mitchell Eaton Biological Science Lab (BIOL 1001-029) February 24th‚ 2015 ABSTRACT: Purpose Statement: HYPOTHESES: Unknown Substance One: Unknown Substance Two: Unknown Substance Three: Unknown Substance Four: Unknown Substance Five: Unknown Substance Six: PROCEDURES: Materials Student Provides: Distilled Water 2 Clean Sheets of Paper 1 Paper Towel 1 Pair
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The unknown soda ash from experiment 3 was used‚ to determine the weight for each trial we used the equation of (M of HCl) x (18 ml x 105.99) / (10 x 2 x Na2CO3 ). Which was equal to (0.01472 M) x ((18 mL X 105.99)(10 x 2 X 2.428 % )= 0.6 g. To start we had to rinse the beakers‚ electrode and the stirring bar with diluted water. The sample we needed was weighted to the closest 0.1 mg which we got was 0.3 for the first trial. The sample was transferred to a 250 mL beaker and dissolved in 70 mL of
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Materials & Methods Brook trout blood was used throughout our experiment as our erythrocyte suspension‚ which consisted of ten drops of fish blood in a test tube containing 10mL of 0.7% NaCl. Eleven other solutions‚ (erythritol‚ xylose‚ monacetin‚ diacetin‚ triacetin‚ urea‚ thiourea‚ glycerol‚ ethylene glycol‚ glucose and fructose) all isosmotic but not necessarily isotonic with the cytoplasm of the erythrocyte‚ were combined with a 0.2mL of well-mixed stock suspension were added to 0.27M of each
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