Erythromycin‚ Penicillin‚ and Ampicillin are affected by the bacteria Serratia marcescens. Our hypothesis is that Penicillin will have the largest ring of inhibition‚ the ring around the disk where the antibiotic has killed bacteria. We believe Penicillin will be the most common antibiotic that we have heard of. The materials we used to make this test possible consists of a sterile nutrient agar Petri dish‚ the bacteria Serratia marcescens‚ Erythromycin antibiotic disk‚ Ampicillin antibiotic disk
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Serratia marcescens is a gram-negative rod shaped bacteria that is ubiquitous in the environment. S. marcescens is a member of the genus Serratia‚ which is a part of the family Enterobacteriaceae (Herra 1). Currently 14 species of Serratia are recognized within the genus‚ eight of which are associated with human infection‚ S. marcescens being the most common clinical isolate and the most important human pathogen of the genus (Herra 1). Serratia marcescens is also particularly prevalent in hospital
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In bacteria‚ temperature‚ pH‚ and other chemical agents all affect the expression of genes. In this lab‚ the effect of temperature change on the gene which codes for a red pigment called prodigiosin of bacterium Serratia marcescens is being tested. Serratia marcescens is usually found in soil and plants‚ and the accumulation of prodigiosin in the bacterial cells makes them appear red. Prodigiosin is produced only at certain temperatures‚ so by regulating the temperature in which Serratia marcescens is cultured
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Experiment 9: Growth curve of Serratia marcescens Abstract Bacteria grows by binary fission. The aim of this experiment is to follow the growth of Serratia marcescens in nutrient broth at 37oCby recording the changes in turbidity (cloudiness) by measuring the absorbance of visible light (600 nm) and also to prove that there is an increase in the cell number and not just in mass during the growth. In the experiment we measure the full growth curve of Serratia marcescens by measuring the absorbance
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Tetracycline and Chloramphenicol against Serratia marcescens By C. A. Bechdel Abstract The problem investigated was Serratia marcescens. We hypothesized that both Tetracycline and chloramphenicol will be effective against treating S. marcescens. The experiment was conducted with a single control group and two experimental groups. The experiment was replicated 8 times. The experiment showed that in testing the control group S. marcescens‚ chloramphenicol had a mean death zone of 30.9 mm +/- 2
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria are unicellular microorganisms‚ with variable shapes and nutritional needs. They lack a distinct nucleus and occur singly or in chains or clusters and form distinct colonies. Bacteria are classified on the basis of many
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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phonetically Dictionary - View detailed dictionary 1. article 1. the The purpose of this is knowing the types of bacteria that exist in our environment and diseases caused by them‚ and how treat it. MATERIAL AND METHODS: An unknown bacterium was handed out
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Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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