• Unknown Paper- Staphylococcus
    nutrient medium, TSA plate isolation streak, Gram stain and spore stain of organisms, general characterization tests, and specialized tests. After collecting all information, a specific bacterium was determined to be of normal flora of the human skin. There are three areas used to identify the...
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  • Micro Lab Pcn
    kit supplied by BD for the Escherichia coli strain according to the standard method of Gram staining. Slides were made and observed using a Compound Light Laboratory Microscope at 1000 magnification using oil immersion. 3.2 Growth on Different Media Escherichia coli was streaked on a TSA plate...
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  • Unknown Lab
    . 2013d. Microbiology in pictures. www.microbiologyinpictures.com Collin, B. 2013. Salmonella-shigella plates. http://iws2.collin.edu/dcain/CCCCD %20Micro/salmonellashigella_plates.htm Frankhauser, D. 2001. Triple Sugar Iron Interpretation. http://biology.clc.uc.edu /fankhauser/labs/microbiology...
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  • Lab Report
    of test in the lab were done. To decipher the two bacteria, the first procedure that needed to be done was to streak two Blood Agar plates (BAP) and a Trypticase Soy Agar plate (TSA) with the given test tube of bacteria. The Quadrant method was used to streak the plates, and this method was used...
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  • Microbiology
    typically used.. For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction, the...
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  • How to Write a Lab Report in Microbiology
    "They" are not typically used.. For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This...
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  • Unknown Microbiology Report
    (TSA) plate. This method was performed as stated in the Leboffe & Pierce lab manual(1). The TSA plates were incubated at 30 degrees Celsius for 48 hours. This process was repeated until isolated colonies appeared. At this point cell morphology was observed and recorded. At this time the unknowns...
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  • Nothing New
    MICROBIOLOGY LABORATORY TECHNIQUES BI 122 LABORATORY TEST #2 - Thursday April 28, 2013 1. SMEAR + GRAM STAIN + MICROSCOPY (from TSA culture plate) i. On the frosted end of a clean microscope slide, with a PENCIL, write the correct labelling information a. Prepare a smears...
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  • Gram Negative Unknown Lab Report
    tube and I inoculated a TSA plate using the T-Streak method in order to isolate the bacteria and also to grow bacteria to use for my tests. Materials and Methods The materials I had available to me for use for this experiment were a TSA plate, a TSA slant, a Gelatin Tube, a Methyl Red Tube, a Voges...
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  • Unknown Microbiology Lab Report
    colonies to be studied, tested and identified. After incubation of the individual TSA plates, the morphologies were viewed and noted and a Gram stain was completed on each individual bacterium, which will be referred to as Bacteria #1 and Bacteria #2. After the Gram reaction was determined on Bacteria...
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  • Anaerobic Bacteria
    things. (Anaerobic Organisms Wikipedia).The thioglycollic acid and agar prevent oxygen from entering the entire medium. The medium used was a plate of TSA +glucose, one incubated anaerobically in a Gaspack jar and another incubated aerobically. When the anaerobic jar had completely depleted the...
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  • Unknowns
    the unknowns was a separation test using a Trypticase Soy Agar Plate (TSA). After the TSA plate was incubated and grown for 24 hours a green hue appeared on the plate. After observing the TSA plate a gram stain was performed on the greenish organism. Necessary steps were taken when conducting the...
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  • How to Write a Lab Report
    should be in a table format (see examples below). This is also where the flow chart showing how you arrived at the answer is stated. A short paragraph explaining how the results are presented can be included. Example: Unknown G had the following morphology on a TSA plate: medium sized opaque...
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  • Identification of an Unknown Bacterial Species
    ) plates. After 48 hours of incubation, it was unclear that two different bacterial colonies had grown on my TSA plate. Only one type of colony was evident. However, it was apparent that I had successfully isolated two different bacterial species by examining my MAC and CNA plates. Only one type of...
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  • Lab Report
    , giving us 3 different dilutions for each microorganism at the respective temperature. Once the dilutions were completed, we performed the spread plate technique. To do this, we took 100 L from each dilution, placed them on a separate TSA plate, and spread the culture with a sterile glass rod. All...
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  • Unknown
    construct a scheme to apply to pure colonies isolated by T-streaking on primary and secondary trypticase-soy agar plates. The morphology of the colonies was noted, and bacteria from isolated colonies were Gram stained and cultured on TSA slants. Gram morphology was the basis upon which it was decided...
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  • Biological Media
    coat and gloves. With my hands sterile and my lab coat on I was ready to begin the lab. On my lab counter were four TSA plates, one EMB plate, one MSA plate, and three SIM tubes. I also had the organisms Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Citrobacter freundii. Using...
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  • MicroOrganism
    of an unknown bacterium. Materials: 1-Tube containing unknown bacteria, crystal violet, iodine, decolorizing agent, Safranin, Bunsen burner, sheep blood agar plate, TSA plate, and bacitracin antibiotic disks. Procedures 1: 1.) Obtain a tube with an unknown bacteria and materials...
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  • Unknow Lab Report
    manual and biochemical test handouts. The first procedure that was done was a gram stain followed by a streak of the unknown on a TSA plate in order to determine the gram reaction and observe the colony morphology. After that, specific biochemical tests were performed for gram positive, since unknown...
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  • microbiology
    TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction, the summary, the description of the materials and methods...
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