• Dioxygen Activation at Mononuclear Nonheme Iron centers
    iron. Prior to 2007, the mechanisms of extradiol dioxygenases were predicted based of spectroscopic studies that could not directly detect the steps after the substrate was bound. However, in 2007, Kovaleva and Lipscomb report three intermediates of the extradiol-cleaving mechanism by crystalizing...
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  • Elisa
    such enzyme is the horseradish peroxidase (as in the experiment) which reacts with the substrate hydrogen peroxide in the presence of the chromogen 5-amino salicylic acid to produce a brown product (School of Science, 2010). There are three types of ELISA formed from the basis which include the...
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  • Bio 110 Exam #2
    oxygen • Start with glycolysis in cytoplasm • Completed in mitochondria Main Pathways Start with Glycolysis • Glycolysis occurs in cytoplasm • Reactions are catalyzed by enzymes Glucose (six carbons) 2 Pyruvate (three carbons) Overview of Aerobic Respiration C6H1206 + 6O2...
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  • Tarek
    recognition? The specificity of an enzyme results from its three-dimensional shape. Only a portion of the enzyme binds to the substrate. The active site of an enzyme is typically a pocket or groove on the surface of the protein into which the substrate fits. e. The active site is an...
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  • Organisms
    accumulate but becomes the reactant in the next step. Sunlight provides a daily source of free energy for photosynthetic organisms. Nonphotosynthetic organisms depend on a transfer of free energy from photosynthetic organisms in the form of organic molecules. A cell does three main kinds of work...
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  • Dopa Concentrations
    cuvette one to cuvette two, the final amount of Dopachrome formed at 200 seconds increased. This same pattern occurred from cuvette two to cuvette three. A reason why the results supported our hypothesis is that when the amount of enzyme, tyrosinase, is kept constant but the amount of substrate, or DOPA...
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  • Biochem Study Guide for Test 2
    activation energy is lower, more molecules have the energy required to reach the transition state and more product will be formed faster. The essence of catalysis is stabilization of the transition state. Formation of an Enzyme-Substrate (ES) Complex is the First Step in Enzymatic Catalysis * Substrate...
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  • Fungal Amylase
    main factors, the main one being temperature. Temperature affects how quickly an enzyme breaks up the substrate. A substrate is the foundation to which an enzyme attaches itself.(Raven, 113) In this experiment, the factor we are testing is temperature, which is also the independent variable. If the...
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  • Amylase Immobilisation
    many years in different industrial processes. Usually, immobilization of enzymes is carried out by three principle means, matrix assisted entrapment of enzyme, adsorption on a solid support, ionic or covalent binding (Swaisgood,1985; Zaborsky, 1973). Entrapment is taken as the most preferable method...
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  • Biochemistry
    enzyme ** root of word defines what the enzyme breaks down (ex. SUCRASE  SUCROSE) o How do enzymes work?  Step 1 - Enzyme and substrate come together @ the active site or the enzyme forming an enzyme-substrate complex  Step 2 - Once substrate binds to the active site, enzyme works to create...
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  • Practice Exam Biochemistry
    saccharolytic bacteria able to to? What are the steps in this? SPLIT SUGARS AND THEN FERMENT THEM STEPS: HYDROLYSE BONDS > SUGARS RELEASED > ENTER BACTERIA What are the three main short chain fatty acids? Which one is important for keeping colonocytes (LI cells) healthy? ACETATE, PROPIONATE...
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  • Chemistry Ert
    Alpha amylase is an enzyme that aids in the breakdown of starch and glycogen to form glucose and maltose through the hydrolyses of alpha linked polysaccharides1. Starch is a complex carbon and is the main energy storage material found in plants and some bacteria. It is a major component of food as...
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  • Photosynthesis
    figured out by the order in which the amino acids are linked together. The link of amino acids then folds around itself to form a secondary structure. When the twisting and folding of the amino acids is complete, it creates the distinctive shape of the active site. The three main steps of the...
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  • Biology Notes
    in three major steps, its significance, and its adaptations to produce ATP with changes in kinds and amounts of reactants. d. understanding biotic and abiotic contributions to ecosystems. Notes Chapter 4 – The Energy of Life Section 4.1 – All Cells Capture and Use Energy...
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  • ethanol
    enzyme is very specific. • What accounts for this molecular recognition? The specificity of an enzyme results from its three-dimensional shape, which is a consequence of its amino acid sequence. • Only a restricted region of the enzyme binds to the substrate. 1 The active site of an enzyme is...
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  • Biology Vce
    be divided into three main steps: * Blood at the top of the nephron * is under high pressure * The following are forced through the glomerulus (capillaries) into the bowman’s capsule * Urea * Salt * Water * The fluid (Filtrate) does not...
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  • Cell Respiration
    function 1. It’s a series of reactions that release energy as they break down sugars and other substances to carbon dioxide and water A. Each step in this decomposition is catalyzed by an enzyme B. Respiration releases free energy by oxidizing sugars or other organic substrates. Some of this energy is...
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  • Micro
    A two-carbon fragment called an acetyl group Complex reaction involves three intermediate steps Catalyzed within the mitochondria by a multienzyme complex Pyruvate dehydrogenase: enzyme that removes a CO2 from pyruvate...
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  • How Cells Harvest Energy
    Substrate-level phosphorylation – phosphate groups are added to substrates in enzyme catalyzed reactions. Enzyme Enzyme P ADP + P Substrate P Product ATP Oxidation of Glucose The complete oxidation of glucose proceeds in stages: 1. Glycolysis 2. Pyruvate oxidation 3...
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  • Grade 12 Biology Notes
    * Oxidation: loses electrons * Reduction: gains electrons Enzymes * Protein catalysts * Speeds up chemical reactions * Active site: Where enzyme binds with substrates * Induced fit model: Enzyme changes shape to fit substrate better * Enzyme substrate complex: Enzyme with...
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