• The Effect of Temperature and Ph on Α-Amylase Enzyme Activity
    effect of temperature and pH on the enzyme activity of α-amylase . In this experiment, the reaction of α-amylase with starch will be carried out at different temperatures and pH values with all other conditions constant. Method Refer to lab manual. Results 1.The Effect of Temperature on...
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  • lab report production of antimicrobial peptide from staphylococcus warneri
    activity was observed at pH 6.0 and temperature 70°C. Key Words: Bacillus sp; solid culture; α-amylase. Alpha amylase (EC3.2.1.1, 1,4alpha-D-glucan-glucanohydrolase) is an extracellular enzyme, which is involved in the starch processing industries where it breaks starch down into simple sugar...
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  • Xylanase
    glucoamylases. α-amylase is a good example of an industrial enzyme, which needs to be stable in high temperature and active in physiological temperatures and pHs when used as detergent, feed additive and is used in production of maltodextrin industry. One of the industrial production organisms of α...
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  • carbohydrates
    amylase. The aim of this practical session is to: 1. Study the effect of salivary amylase on starch. 2. Study the effect of different factors (such as temperature, pH and time) on the activity of salivary amylase. 2 Practical In a beaker, collect about 1 ml of your own “saliva” then add 9...
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  • Amylase Production by A.Niger Under Solid State Fermentation Using Agroindustrial Waste
    minute per milligram protein (U/mg). 2.6.1. Effect of Incubation Time: The effect of incubation period on enzyme production was investigated by checking the enzyme activity on 4th, 5th, 6th, 7th and 8th days of incubation in the different solid substrates at pH 7 and at room temperature. 2.6.2...
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  • enzyme activity
    circumstances both. This analysis is expedited by the accessibility of purified enzyme preparations, which can be mixed with the correct purified substrate. This experiment will examine the changing effects that the enzyme Amylase has on the substrate Starch when environmental factors such as pH, temperature...
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  • Aspergillus Niger
    digestive enzyme, and its optimum pH is 6.7-7.0 (Abu EA et al, 2005) In human physiology, both the salivary and pancreatic amylases are α-amylases. They are discussed in much more detail at alpha-Amylase. This form is also found in plants, fungi (ascomycetes and basidiomycetes) and bacteria (Bacillus). It...
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  • Salivary Amylase
    of salivary amylase is highest at pH 7 – pH of oral cavity is close to 7  At pH 8, – decrease in activity  acidic pH 3, 4 and 5 – enzyme acitivity was at minimum Page  37 Effect of Temperature • reaction rate of an enzymatic reaction increases as the temperature is raised • 10...
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  • effect temperature has on amylase
    The effect temperature has on the activity of the enzyme α- amylase. Introduction Enzymes are a biological catalysts, which means that they speed up the chemical reactions in living organisms. Almost all of enzymes are energized protein molecules that catalyse and regulate nearly all biochemical...
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  • What!!!?
    Objectives: 1. Associate the presence of enzymes with the catalysts of the chemical reaction in living cells. 2. Determine the effect of enzyme concentration, substrate concentration, temperature, pH and heavy-metal salts upon the activity of salivary amylase. Results: A. Enzyme...
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  • Bio Lab Report 1—— Enzyme
    Ye Tao BISC220-13155 The Effect of Temperature on the Digestion of Starch by Activity of Enzyme α-Amylase: Observation of Rate of Starch Disappearance through Iodine Test Introduction An enzyme is a type of protein that, through its own structure including hydrogen bonds, acts like a...
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  • Starch Hydrolysis by Amylase
    tested [5]. Starch + I2  blue-black color Amylase enzyme Proteins which catalyze the chemical reactions are called enzymes. Non-biological catalysts work at wide ranges of temperature and pH, whereas enzymes must work in mild conditions in cells, between 32 0C and 40 0C and at a pH between 6.5...
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  • Biotechnology
    substrate, α-amylase tends to be faster-acting than β-amylase. In animals, it is a major digestive enzyme and its optimum pH is 6.7-7.0. [1] In human physiology, both the salivary and pancreatic amylases are α-Amylases. They are discussed in much more detail at alpha-Amylase. Also found in plants...
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  • Lab Report Digestion
    | Amylase―――――――→ | Disaccharides(maltose, sucrose, lactose) | Ptyalin has an optimum pH of around 6.8, which is roughly the pH found in the mouth. Protein digestion begins in the stomach (gastric digestion) where the enzyme pepsin splits proteins to shorter polypeptide chains containing amino acids...
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  • Biological Activity of Actinomycetes
    able to produce Amylase enzyme but lack in producing other enzymes like beta-Glycosidase, Chitinase. Our work can be taken forward to study many more biological activities. Also each activity we study is a complete project in itself and our results promise for application of our isolates in Amylase...
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  • Investigation of Effect of Temperature on Amylase Activity
    Investigate the effect of temperature on amylase activity Introduction Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants, animals and microorganisms. Large amounts of amylase occur in germinating cereals, and in the pancreas and...
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  • Bio Unit 1 Notes
    enzyme-catalyzed reaction can be saturated – all available specific enzymes are bound to their substrates ·         there are a limited number of enzyme molecules that can be used at any one time ·         both temperature and pH affect enzyme activity ·         Figure 5a, p. 72 illustrates how...
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  • Biology
    concentration [4] © A.P 9700/02/REV/2014 jfkhan04@gmail.com For Examiner’s Use November 2009 paper 2 2 Amylase is an enzyme that catalyses the hydrolysis of starch. A student investigated the effect of pH on the activity of the enzyme. Eight test-tubes were set up each...
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  • Microorganisms Are Used Extensively to Provide a Vast Range of Products and Services.
    , Standardization of enzyme activity. 5 Effect of pH, temperature & substrate concentration on invertase activity. 6 Partial purification of invertase enzyme from Penicillium chrysogenum & Penicillium brevicompactum. 7 Determination of protein & specific activity of invertase. MATERIALS & METHODS...
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  • Enzyme
    100 years ago. The enzymes used are mainly of bacterial origin such as Bacillus subtilis. Owing to advances in biotechnology a range of amylases acting at different temperatures from 20°C up to 115°C is available today. The optimum pH of the treatment lies between 5 and 7, depending on the enzymes...
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