Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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of great importance to recognize and identify the different instruments and laboratory tools‚ because in this way will we be able to use them properly and also to call them by name and know why. Listen Read phonetically Dictionary - View detailed dictionary 1. article 1. the The purpose of this is knowing the types of bacteria that exist in our environment and diseases caused by them‚ and how treat it. MATERIAL AND METHODS: An unknown bacterium was handed out
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Having the ability to collect and identify unknown microorganisms is vital in health and medicine. This capability is important for a variety of reasons‚ such as knowing the causative agent of disease‚ knowing if the microorganism obtains any beneficial properties and knowing the correct microorganism to use to create a successful antibiotic. Implementing the experimental methods learned thus far in the microbiology laboratory allowed an unknown bacterium to be identified as a result of this study
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classified uniformly in order to easily categorize and identify organisms. This classification‚ or taxonomy‚ uses the genus name followed by the species name‚ in Latin. By having a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria are unicellular microorganisms‚ with variable shapes
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Introduction Although bacteria is microscopic in size‚ it is largely important in the healthcare field‚ environmental work‚ food preparation‚ as well as many other industries. In particular‚ it is essential that healthcare workers be able to identify the species of bacteria invading a human reservoir in order to prescribe the correct antibiotic that will kill that species. For the purpose of bacteria identification‚ numerous tests have been devised to find out the exact species in question. However
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The unknown bacteria A and bacteria B have to be identified by its genus and species. First both bacteria had to be inoculated into a TSA agar media using the streak plate method. Four quadrants were drawn‚ so that the bacteria could be isolated as much as possible. Each bacteria was inoculated into two different plates‚ so that one could be incubated at 37 degrees Celsius and the other at 25 degrees Celsius. Bacteria B‚ which was incubated at room temperature showed red colonies throughout its media
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Determining the Unknown Microorganism from a Mixed Culture Mylah Ferland‚ Rivier University MICRO 206‚ March 16th‚ 2016 Introduction: An unknown bacterium experiment‚ this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al.‚ 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by
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Antony R 9:00Am-11Am Unknown Number 94 Unknown Results Streptococcus salivarius The first step in my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram
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species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media can be used to culture and identify bacteria. Some
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the study of microbiology‚ the need to identify an unknown microbe may arise. This aids in the recognition of which microbes are harmful or pathogenic as well as those that may be beneficial to us in some way. This report will detail how a similar project was performed in order to identify two unknown micobes using the techniques that were learnt during the course of the semester. We embarked on the project with the knowledge that we would have to identify a Gram(+) as well as a Gram(-) from the
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