Lab 4: Photosynthesis and Chromatography of Spinach Leaves Discussion: The chromatography of the spinach leaves was taken to establish what pigments were present in the chloroplasts of the leaves. The solvent carried and separated the different pigments up the cellulose paper. The solvent moved up the paper because of capillary action because of adhesion of the solvent molecules and the paper. The pigments dissolved in the solvent also were carried up the paper and were separated because
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Effects of Photosynthesis of Spinach Leaves in Different Light Conditions Introduction Photosynthesis is the process of converting solar energy‚ carbon dioxide (CO2)‚ and water (H2O) into carbohydrates (CH2O) and oxygen (O2). Sometimes the end product of photosynthesis is glucose (C6H12O6). Photosynthesis takes place in the chloroplasts of plant cells. Here membranous thylakoids are stacked in grana surrounded by the stroma. During the light reactions‚ pigments within the thylakoid membranes
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CH 223 - Organic Chemistry Laboratory I Experiment #6 - Column and Thin-Layer Chromatography (TLC) of a Spinach Extract Pre-Lab Assignment (1) List 5 ways that TLC can be used in an organic chemistry lab experiment. To identify an unknown‚ to monitor the course of a reaction and assess the purity of its product‚ to determine the best solvent for a column chromatography separation‚ to determine the somposition of each fraction from a column chromatography separation‚ and to determine whether
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In this experiment spinach‚ DPIP‚ phosphate solutions were placed under various filters and the percent orange light transmittance through the solution was measured to determine the rate of photosynthesis. This experiment was set up to discover how different wavelengths of light affect the rate of photosynthesis in a spinach‚ DPIP‚ phosphate solution. I proposed that the spinach‚ DPIP‚ phosphate solution under no filter‚ and therefore exposed to all light will have a highest percent orange light
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Lab Report #1 Photosynthesis Lab Report: The Effects of Varying Concentrations of CO2 on the rate of Photosynthesis in Spinach Leaves Introduction Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct. The purpose of this experiment is to see how different concentrations of carbon
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pigments found in spinach plants and determining the spectrum of light each absorbs. By using paper chromatography‚ different pigments of spinach leaves can be separated. The knowledge gained in this experiment is relevant to understanding how the process of photosynthesis works. A Real-world application for this includes the harvesting of clean energy sources‚ as scientific advances have led the way to artificial photosynthesis on the path to replace fossil fuels (Nath‚ 237). Photosynthesis converts light
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BIOLOGY DCN PHOTOSYNTHESIS LAB # 6 Effect of varying coloured filters on the photosynthetic rate of spinach chloroplasts The data below is just representative of what trends and relationships you were supposed to see. Numbers can vary. Absorbance at 620 nm for each treatment DCPIP + chloroplasts t=0 min. 0.93 0.945 0.905 0.915 t=3 min. 0.95 0.731 0.83 0.816 change in A620 -0.02 0.214 0.075 0.099 t=0 min. t=3 min. change in A620 t=0 min. t=3 min. change
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take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used‚ starting with the least polar‚ to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography. Procedure: The first part of the experiment dealt with breaking down the spinach leaves
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Absorption spectrum of the Spinach Chloroplast By a Different Wavelength and Photosynthesizes Activity in different light filters Instructor: Dr‚ David Danseraveu Student: Hassan Sedaghat‚ A00345910 Due date: 11/13/2012 Material and Method: In the photosynthesis system‚ plants and other photosynthesizing creatures they use light of the sun as energy by absorbing some specific wavelength. A prepared sample of the chloroplast from spinach was used during this experiment
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What Factors Affect the Rate of Photosynthesis in living leaves? Abstract: In this lab‚ four different types of leaves were tested to see the rate at which each leaf photosynthesized. This lab demonstrates how plants store light‚ capture light‚ and use light as energy for reproduction and growth‚ by photosynthesis. The control in this experiment was spinach‚ which was tested before any of the other plants were. The other plants that were tested were English Ivy‚ C4 Plant‚ and a multicolored
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