Self-design Practical: The effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas‚ where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes‚ including amylase. As the pH level increases‚ the enzyme activity
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in neutral pH. To do this the experiment was carried out so that tubes containing a reaction solution of the Amylase enzyme and starch were simultaneously mixed. The reactions were then introduced to I2-KI‚ which stopped the reactions‚ at two minute intervals. Each of these trials was repeated three times to ensure proper accuracy. After concluding the reactions they were placed into a spectrophotometer (A580) for analysis. Graphing the values of the absorbance to time for each pH it was found
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Testing the pH of Common Household Substances Lab Background Information: The pH scale is used to determine the acidity or basicity level of liquid solutions. If a solution scores a pH level of 1-6 it is an acidic solution‚ 1 being the most acidic and 6 being the least acidic. If a solution rates a pH level of 7 it is a neutral solution‚ meaning it is neither an acidic nor a basic solution. Lastly‚ if a solution is 8-14 on the pH scale it is a basic solution‚ 8 being the least basic and 14 being
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Biochem. J. (1995) 305‚ 17-20 (Printed in Great Britain) 17 RESEARCH COMMUNICATION The effect of low temperatures Nicole MORE‚ Roy M. DANIEL* and Helen H. PETACH on enzyme activity Thermophile Research Unit‚ University of Waikato‚ Private Bag 3105‚ Hamilton 2001‚ New Zealand The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain ANI and f‚- enzymes‚ glucosidase from Caldocellum saccharolyticum expressed in Escherichia
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pH indicators‚ in chemistry‚ are usually a weak acid or base that are naturally occurring in various plants and flowers and can act as dyes. Some examples of indicators are Litmus (Comes from a plant species called a lichen)‚ Phenolphthalein‚ Thymol blue‚ Bromocresol green and Universal indicator. All these indicators have a certain turning point in color once a certain concentration equilibrium of Hydrogen cations in reached (H +) or a certain concentration equilibrium of H3O+(Also OH - using Arrhenius
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Factors that affect how the PH changes during a titration experiment. Concentration of the alkali Having a higher concentration of alkali will mean that there will be more molecules closer together for the acid to collide with. This will speed up the reaction as collision theory suggests that molecules have to collide to react and if there are more molecules to collide with the reaction will happen faster as there is a higher chance of a collision. Volume of alkali An increase in volume would mean
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Effects of Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes‚ proteins that act as catalysts‚ are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes‚ the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
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Does the PH level of acids in our stomach affect the digestion proteins? Yes‚ if the acidity in my/a stomach is high in terms of the PH balance then the proteins in our stomach will be digested faster because acids are known to breakdown and eat away at many objects. The purpose of this project was to observe how the digestion of protein varies at different pH levels. This project in my eyes helps society by finding the best types of food (i.e. ones with higher/ lower acidity and pH balance)
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determining the effects of pH on enzyme activity show that as the independent variable‚ pH‚ increases the dependent variable‚ percentage transmission‚ decreases. This is shown in the results as at the lowest pH‚ pH 4.0 the average percentage transmission is at its highest at 97%. At the highest pH‚ pH 8.0 the average transmission is 78.5%. This is also supported from the graph as it produces a negative gradient showing that as the percentage transmission will decrease with an increasing pH. This happens because
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The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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