plasmid DNA and analysis of isolated plasmid Introduction: A plasmid is an autonomously replicating extra-chromosomal genetic element. In other words‚ this is a DNA molecule external to the bacterial chromosome that is able to replicate on its own and distribute its daughter molecules to daughter cells. You have successfully cloned a fragment of chromosomal DNA containing a tetracycline resistance cassette into a plasmid (pET11a). To this end you have (1) isolated total chromosomal DNA from your
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LABORATORY REPORT BIOLOGY CELL AND GENETICS (SB10203) TITLE: DNA Extraction from Banana DATE OF LABORATORY: 03/05/2013 LECTURER’S NAME: DR. LAM NYEE FAN DEMONSTRATORS’ NAME: MISS NOR EZANI AHMAD MISS LUSIA BAREK MOSES LABORATORY ASSISTANT NAME: MISS ROSILAH MOHD IDRUS STUDENT NAME AND MATRIC NUMBER: ELYAS ERIC HUIL(BS12110134) BONG SIN NENG(BS12110054) EDILAH NADRAH JOHANY( DIASSOFIA PAULA FRANKIE INTRODUCTION DNA is present in the
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patient. However‚ we are only interested in the patient’s head and more specifically the brain‚ that is why in this step we perform an orientation step to orient all the brain images into a common coordination and then we perform the brain extraction using brain extraction tool (BET) which will result two images‚ one image contains only the brain and the other one contains the brain and the skull‚ both images are needed for further analysis‚ Figure 2.4 and Figure 2.5 Show the MRI image before and after
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DNA Extraction Lab 1/17/14 Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. Hypothesis: If the lab is conducted properly then we should be able to view a visible amount of DNA from the strawberry and detergent mixture. Variables: The independent variable in this experiment is the strawberry mixture while the dependent
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DNA FINGERPRINTING LAB REPORT DNA contains genetic material and information that makes up each individual trait. Every person can be identified by providing his or her genetic information based on a particular DNA strand. DNA information is an effective way of identifying persons if it is used properly. It is used to identify humans in different situations such as crime scenes‚ accident scenes‚ paternity testing‚ soldier remain identification‚ inheritance claims‚ missing person investigations‚
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Experiment 2: Extraction – Evaluation Summary Labs reports must be typed and chemical structures must be drawn with ChemDraw. Report must not exceed three pages (including this page). Page limit does not include any attached spectra or references. ** Deductions for hand written report/structures and exceeding page limit ** Report Breakdown Data/Results: _________________ /10 Discussion: _________________ /10 Report Total: _________________ /20 Other Lab Marks Performance:
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DNA replication mechanism is an integral and the most important part of cell division. It is a complicated mechanism that requires a lot of steps and different enzymes. Many scientists were trying to understand the mechanism of DNA replication and its role in the transfer of the hereditary information to the offsprings. DNA replication begins with a single origin of replication that starts off the process by creating a replication bubble. In many eukaryotic cells‚ the DNA strand will have more than
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and analyzed various DNA fragments in order to determine if these DNA fragments originated from the same individual. The learning objective for this lab is to gain a better understanding of how DNA fingerprinting works. In this lab the primary function is to determine which DNA fragments match the DNA fragment found on the crime scene. To determine if any of the DNA fragments match the fragment found at the crime scene‚ the DNA fragments must undergo the DNA fingerprinting. DNA fingerprinting causes
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The purpose of module E is to learn several DNA techniques in the lab including DNA purification with solubility and absorption‚ plasmid transfection of E.coli‚ colony screening by PCR and quantitative PCR. First part of the experiment E1 show the purification method of DNA through solubility. E. coli lysate mixed organic solvents to purify the DNA present in solution. First‚ the lysate was mixed with phenol/chloroform‚ then vortexed‚ and centrifuged. We extracted the aqueous layer and combined
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this lab was to troubleshoot causes related to PCR components and develop an experiment that would test if the Taq amount is suitable for the PCR reaction to run correctly. INTRODUCTION Thermus aquaticus (Taq) DNA Polymerase is a bacterium that lives in thermophilic conditions and has been identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR (Noronha & Mullins‚ 1992). Why might you not be getting any bands on your PCR? If the Taq DNA polymerase
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