"Identifying Bacteria On Agar Plates" Essays and Research Papers

Identifying Bacteria On Agar Plates

DIFFERENT TYPES OF AGAR Mac Conkey‘s Agar plate MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria, except Enterococcus and some species of Staphylococcus), crystal violet dye (which also inhibits certain Gram-positive bacteria), neutral red dye (which stains microbes fermenting lactose), lactose and peptone. QUALITY CONTROL Results after 24 hrs at...

Bacteria, Enterobacteria, Enterobacteriaceae 1285  Words | 7  Pages

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The Role of Agarase in Agar-Degrading Bacteria

The Role of Agarase in Agar-Degrading Bacteria Abstract Agar-Degrading (agarolytic) Bacteria is physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii, regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods, optimum conditions and the...

Agar, Agar plate, Bacteria 1838  Words | 6  Pages

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Unknown Bacteria

having a universal method of identifying bacteria allows for all scientists from any part of the world to identify the same species in an identical manner allowing for a precise of classification. Bacteria are distributed throughout the world in almost every conceivable habit. Bacteria are unicellular microorganisms, with variable shapes and nutritional needs. They lack a distinct nucleus and occur singly or in chains or clusters and form distinct colonies. Bacteria are classified on the basis of...

Bacteria, Bacteriology, Gram staining 1832  Words | 5  Pages

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Methodology By using aseptic, a little cultured bacteria was inoculated on the TSA agar. A quadric streak was making. Inoculation loop was heated and keep it cold for a while before the next quadratic streak. Six agar plates were observed for 24 hour at temperature of 30ºC. Choose one from the dense colony and make a sub-culture on the new agar plate. The step was repeated to get a single colony, which is pure colony. a) Sequestration of bacteria from fish organs Methodology Dissecting set...

Agar plate, Growth medium, Laboratory equipment 644  Words | 3  Pages

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Examination of Bacterial Plates

Bacterial Plates Objective: 1. To examine bacteria pigmentation, colony, margin characteristics, elevation properties, broth characteristics and agar stroke properties. 2. To examine bacteria growth characteristics on different culture media. Introduction: Bacterial species can sometimes be identified on the basis of how they appear on or in the different media. The pigmentation, size and shape of bacterial colonies as they grow on and in agar plates can provide identifying signs. Observing...

Agar plate, Bacillus, Bacteria 736  Words | 5  Pages

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Streak Plate

Instructor: Dirk VandePol Date: 6/21/2013 Streak Plate Isolation for Obtaining Pure Culture 1. When an agar plate is inoculated, why is the loop sterilized after the initial inoculation in put on? Ans: We use agar plate to inoculate microbes by zipping the loop on the agar several times. We streak on the agar plate four time, propose is to isolate the unknown bacteria. Therefore, the first time to streak on the plate, there are million of bacteria on the loop. For that reason, we need to sterilize...

Agar, Agar plate, Archaea 657  Words | 3  Pages

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Anaerobic Bacteria

Livja Koka Anaerobic Bacteria In this experiment the purpose is to seek understanding between an organism and its use of oxygen. Oxygen is a very important part of the organisms’ survival. Oxygen has a tendency to form very reactive by-products inside a cell. These by-products create havoc by reacting with protein and DNA, thus inactivating them. Cells that are able to live in the presence of oxygen have evolved enzymes to cope with H2O2 and O2- and thus are not inhibited by O2. Also many anaerobes...

Anaerobic organism, Bacteria, Cellular respiration 769  Words | 3  Pages

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microbiology final exam unknown bacteria report example

inoculating loop and dry heat technique, isolation streak was performed and nutrient agar plates were to be incubated in a room temperature for the next 48 hours. Nutrient Agar plate was used for isolation streak technique in order to see two types of bacterium growing in a room temperature. After incubating for 48 hours Nutrient agar plates were examined for bacterial growth of two different colonies. On a Nutrient agar plate two different cultures were observed. In order to proceed identification, those...

Bacteria, Bacteriology, Gram staining 1749  Words | 11  Pages

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Bacteria and Plasmid to Produce Red Fluorescent Proteins

Bacterial Transformation Bacteria and plasmid to produce Red Fluorescent Proteins Alejandra Lopez ...

DNA, Escherichia coli, Gene 2536  Words | 7  Pages

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could achieved of which different products to test for my concluding idea. The type of bacteria that I am going to discuss and chosen is E-coli. I will also going to research the effectiveness of antibacterial cleaning products, for instance sanitizer. I will also, research which is the most effective product for the house hold and some other work places. Background Information What are Bacteria? Bacteria are found in: soil, radioactive waste, water, plants, animals, deep in the earth's crust...

Antibiotic resistance, Bacteria, Diarrhea 897  Words | 3  Pages

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|Appearance of Colonies |Microorganisms | |Red, surrounded by reddish precipitation zones, diameter1- 2 mm |Lactose-positive Enterobacteriaceae: coliform bacteria, E. coli | |Pink, pin-point colonies |Enterococci, possibly Klebsiella | |Colorless |Lactose-negative Enterobacteriaceae...

Bacteria, Coliform bacteria, Enterobacteriaceae 276  Words | 3  Pages

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disinfectant and antibiotics effect on bacteria method

minutes so it can penetrate the harder capsules of some bacteria. 4. Using a sterile pipette with draw some of your bacteria culture from the bottle making sure you flame the neck of the bottle to keep the procedure sterile. Place what you have withdrawn on to an agar plate. 5. then get your glass sterile spreader out of the packet making sure you only touch the handle and with the spreader spread the desired bacterial culture on an agar plate, making sure the lid of the culture container does not...

Antiseptic, Bacteria, Combustion 936  Words | 2  Pages

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Ubiquity of Bacteria

Microorganisms can be prokaryotic—the bacteria or eukaryotic—the algae, protozoa or fungi. While viruses are acellular they are also studied in the scope of microbiology because they are small and because they infect cells. While most bacterial are unicellular they can also exist in colonial or multicellular forms. In this laboratory exercise you will examine the ubiquity and diversity of various microbes that are present in the environment or inhabit the human body. Most bacteria that inhabit the body are...

Ascomycota, Bacteria, Eukaryote 1755  Words | 6  Pages

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Effect of Spices and Herbs on Inhibiting Bacteria Growth

covered nutrient agar? Hypothesis: If 3 teaspoons of chopped cinnamon, cloves, mustard, ginger, and garlic steep in half a cup of boiling water for 15 hours and then placed in nutrient agar covered with Staphylococcus epidermis (gram positive) bacteria for 24 hours (in an incubator), garlic will have the strongest antimicrobial effect. This is because of allicin (an active principle of garlic homogenates), which has a chemical reaction with thiol groups of enzymes in the bacteria. Ginger will have...

Antimicrobial, Distillation, Distilled water 775  Words | 4  Pages

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Agar Plate

Laboratory: a. Can be classified according to: a.i. Consistency: a.i.1. Solid Media (contains 2% agar): for colony morphology, pigmentation and hemolysis. a.i.1.a. Nutrient Agar a.i.1.b. Blood Agar a.i.2. Liquid Media (contains no agar): for inoculum preparation, blood culture, and for the isolation of pathogens from a mixture. a.i.2.a. Nutrient Broth a.i.3. Semi-solid Medium (contains 0.5% agar): a.i.3.a. Sulfide-Indole-Motility Medium (SIM Medium) a.ii. Constituents (ingredients): a.ii...

Agar, Agar plate, Eyepiece 5586  Words | 18  Pages

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Unknown Bacteria Lab Report

Introduction The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period, eight prepared types of test media were provided to...

Bacteria, Bacteriology, Escherichia coli 1467  Words | 5  Pages

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Biology agar plates

the virus from the host’s nuclease enzymes. Spikes on the envelope provide a binding site for attachment to the host. Influenza virus is an enveloped virus with many glycoprotein spikes. Viruses are larger than prions and many times smaller than bacteria. They range in size from 30 to 300 nm. Cause: The causes of the flu are from three types of influenza viruses: A, B and C. Human influenza A and B viruses cause seasonal epidemics of disease almost every winter. Influenza type C infections cause...

Avian influenza, Human flu, Immune system 2164  Words | 6  Pages

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Lab: Sampling Bacteria

Sampling Bacteria Purpose: Refer to handout sheet. Materials: Refer to handout sheet. Procedure: Refer to handout sheet. Pre-Lab Questions: 1. Why is one dish being reserved for the class as a "control"? Having a controlled variable is important in order to be able to look at what the bacteria would look like if it hadn't been contaminated and just left as agar. Having a sample of agar that wasnt exposed to any bacteria will provide a clear picutre of what grew on the agar upon feeding...

Agar plate, Bacteria, Bacteriophage 809  Words | 3  Pages

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Agar Plate and Cobra Vine Plant

were presented. In the first experiment (solvents Methanol and Ethyl Acetate), within twenty minutes observation period after injection of the venom, the muscle became less responsive to the four volts stimulus as shown in the decreasing amplitude (Plate 8). Twenty minutes after injection of the cobra toxin, the antivenin was injected. The muscle, which was almost paralyzed, contracted vigorously that brought back even higher than normal contraction of the muscle, as shown in Fig 1. The second experiment...

Agar plate, Flower, Mister Hyde 1258  Words | 4  Pages

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Identifying Salmonella Choleraesuis

2012 Identifying Salmonella choleraesuis using Several Microbiology Lab Techniques The purpose of this study was to determine what an unknown bacteria was using several different microbiology lab techniques including an API test, an oxidase test, a gram stain, a hanging drop slide, and morphology identification. The unknown bacterium, which was contaminated with Serratia marcescens, was isolated by streaking the bacteria solution to single colonies. The isolated unknown white bacteria, had the...

Bacteria, Bacterial cell structure, Bacteriology 1410  Words | 4  Pages

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Identifying unknown bacteria

fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria, gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The SIM test, which tests for sulfur reduction, indole production, and motility, MRVP which is a test used to distinguish...

Bacteria, Bacteriology, Escherichia coli 3351  Words | 22  Pages

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Aerobic Plate Count (Apc) and Total Coliform Count of Supplied Yogurt Sample.

Experiment No. 03 Date: Name of the experiment: Aerobic plate count (APC) and total coliform count of supplied yogurt sample. Purpose: To determine the aerobic plate count and total coliform count of the supplied yogurt sample. Principle: Yogurt squeezed or extracted from milks are more or less acidic, depending on the product, the pH generally ranges from about 2.4-4.2 and contain sugar (4.7g), fat...

Acetic acid, Acid, Bacteria 546  Words | 3  Pages

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Isolation of Bacteria

Pillay, Esmerelda (209504371) School of Biochemistry, Genetics and Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us, and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests, an isolated bacterium can be identified. In this experiment, a single bacterial colony was isolated form Mycorrhizal spores, and further...

Bacteria, Carbohydrate, Enzyme 805  Words | 3  Pages

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Klebsiella Bacteria

A very important technique in Microbiology laboratories is identifying unknown bacteria cultures. Unknown identification is used for practical purposes, such as diagnosing diseases or determining treatment of an infection. Microbiologists identify unknown bacteria to determine new and emerging infectious diseases and current diseases to determine if any kind of treatment or antibiotics can be used. Some bacteria do not have distinguishing morphological features, so there are different kinds of...

Bacteria, Cell wall, Gram staining 2407  Words | 7  Pages

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Micro Lan Unknown Bacteria Salmonella

Title: “Identification of Unknown #1” Purpose: Discovering and studying new bacteria can lead to new innovations and possible cures for existing or future diseases. The investigation of bacteria can also help incubate ourselves or act quickly in response to an invasive strain. The commercial food industry has a great need in identifying Salmonella to prevent disease in consumers. As well as being the cause of enteric (typhoid) fever, an important infectious disease, Salmonella is perhaps best...

Bacteria, Escherichia coli, Foodborne illness 813  Words | 3  Pages

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Oxygen Requirements of Bacteria

Oxygen Requirements of Bacteria BACKGROUND  The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms.  A candle jar is useful...

Anaerobic organism, Bacteria, Carbon dioxide 1019  Words | 4  Pages

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The Effects of Black Tea on the Growth of E. Coli Bacteria Cultures

tea on the growth of E. coli bacteria cultures Kierstin Barker, Melissa Bischak, Jackie Tyszkiewicz, Errin Enany September 25, 2012 Abstract: This study was carried out in order to investigate whether black tea has antimicrobial properties as stated in Steven Johnson’s The Ghost Map. If tea does have antimicrobial properties, then it could aid in warding off waterborne diseases. We believed that if black tea is steeped in boiled water, then the amount of bacteria exposed to this solution would...

Antimicrobial, Drinking water, Escherichia coli 2032  Words | 6  Pages

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Bacteria Lab Report

al., 1966) Therefore, extreme care is required to meet to the standards. To begin meeting the standards, a Mueller-Hinton agar is used for the bacteria culture. The standard Mueller-Hinton agar usually requires a pH between 7.2 and 7.4, which is then poured to a depth of 4mm in either 150 mm or 100mm Petri dishes. The petri dish is then heavily inoculated with a specific bacteria and different paper disks that contain different antibiotics to examine. With the disks on the inoculated petri dish, the...

Agar plate, Antibiotic, Bacteria 1734  Words | 7  Pages

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Unknown Bacteria

Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through...

Bacteria, DNA, Gene 445  Words | 2  Pages

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BACTERIA Period: 4 Characteristics: 3 major shapes Cocci Basilli Spirilla 3 major components Mesosomes flagella Plasmids Growing Up: Bacteria can obtain energy through phototrophs(sunlight), lithotrophs(inorganic compounds), and organotrophs(organic compounds) Marriage/Reproduction Binary Fission: The process by which all bacteria reproduce. It results in the separation of a single cell into two. Transformation: genetic alteration...

Archaea, Bacteria, Cell 725  Words | 3  Pages

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Lab: the Bacteria Around You

Lab: The Bacteria Around You James Brunet Ms Owen October 14th, 2012 Part 1 Purpose To culture and observe the various types of bacteria found around Canterbury High School. Materials and Methods Refer to pages 422-425 of Biology 11 McGraw-Hill Ryerson and the handout “Gram Staining Procedure”. Observations Table 1: Locations of Bacteria Samples Quadrant | Location of Sample Obtained | 1 | Floor | 2 | Water fountain head | 3 | Auditorium Chair | 4 | Inside of Boys’ Bathroom...

Antiseptic, Bacteria, Bacterial cell structure 1092  Words | 4  Pages

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The Structure of the Earth and Plate Tectonics Structure of the Earth Mantle • The Earth is made up of 3 main layers: Outer core Inner core – Core – Mantle – Crust Crust The Crust • This is where we live! • The Earth’s crust is made of: Continental Crust Oceanic Crust - thick (10-70km) - buoyant (less dense than oceanic crust) - mostly old - thin (~7 km) - dense (sinks under continental crust) - young How do we know what the Earth is made of? • Geophysical surveys: seismic, gravity,...

Convergent boundary, Earth, Lithosphere 667  Words | 37  Pages

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Gram Positive Bacteria & Antibiotic Resistance

Sampling of Gram-Positive Bacteria and Antibiotic Resistance Resting We thought it would be interesting to find out the different bacteria that grow on things we use on a daily basis and the level of antibiotic resistance that those bacteria have. We chose to swab the toilet seat in the MATC women’s bathroom and the ATM machine in the commons area on campus. We were certain the ATM would be dirtier than the toilet seat since the bathrooms get cleaned every day. The first step we took in testing...

Antibiotic resistance, Bacteria, Bacterial cell structure 1183  Words | 2  Pages

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Observation: Bacteria on a Chicken Piece and a Handrail, and the Effect of GermX and Alcohol on Them

Observation of Bacteria on Chicken and Handrail and the Effect of GermX® and Alcohol on Them. Sujit Bhimireddy, Daryl Onwuchuruba, Naveen Sood, and Manasa Tripuraneni Biology 106 Abstract Bacteria are found everywhere on this planet. This experiment is aimed at observing some of the bacteria that surround us in places that we use everyday. We will look at bacteria found on a piece of chicken and bacteria found on a handrail. The effectiveness of antibacterial substances such as GermX® and...

Bacteria, Bacteriology, Chloramphenicol 1235  Words | 4  Pages

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Identification Od Unknown Bacteria

The Identification of Two Unknown Species of Bacteria in Tube #72 Introduction: There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of an...

Bacteria, Bacteriology, Enterobacteriaceae 788  Words | 3  Pages

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The Effects of Using 15 Seconds and 60 Seconds When Washing Hands on Different Bacteria

Jennifer Camarata June 5, 2013 Micro-lab Report #1 Mrs. Reilly The Effects of Using 15 Seconds and 60 Seconds When Washing Hands on Different Bacteria The human body contains resident and transient flora, both on and in the body. Resident flora is exactly what it says; it resides on or in the body. Typically this flora is on the surface of the skin, mucous membranes and respiratory tract. In a typical healthy body, resident flora causes no harm while feeding on cellular waste and dead cells...

Antibiotic resistance, Hand washing, Health care 698  Words | 3  Pages

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Bacterial Contamination April 15, 2013 Bacteria Contamination The definition of bacterial contamination is food contamination refers to foods that are spoiled or tainted because they either contain microorganisms, such as bacteria or parasites, or toxic substances that make them unfit for consumption This is very serious and people should take more precaution, food contamination is a serious because it results in foodborne diseases that each year affect an estimated seventy-six million...

Bacteria, Escherichia coli, Escherichia coli O157:H7 877  Words | 3  Pages

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Using Kanamycin Resistance Bacteria to Find the Sources of Contamination of Three Chicken Farms

Using Kanamycin Resistance Bacteria to find the Sources of Contamination of Three Chicken Farms Introduction: Kanamycin is a common antibacterial that interferes with bacterial growth, by inhibiting protein synthesis, and causing the mistranslation of mRNA. Kanamycin is commonly used in chicken feed to keep harmful bacteria from getting into the eggs and producing healthier chickens. Recently reports of severe gastroenteritis have been linked to eating raw or undercooked eggs. This has led to...

Antibiotic resistance, Bacteria, Bacterial conjugation 1979  Words | 5  Pages

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Microbiology Lab Report

Introduction ! Identifying an unknown bacteria can be an arduous journey. The journey starts out by performing a gram stain and then performing various tests. The first part should not be challenging. The challenging part is analyzing your results and using the data to infer what your unknown organism might be. ! When I was first handed my organism, I performed a rather unconventional test: I smelled it. Throughout the semester of dealing with various organisms, I had learned that...

Bacteria, Enterobacteriaceae, Escherichia coli 767  Words | 4  Pages

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Manipulation of Bacteria

MANIPULATION OF BACTERIA INTRODUCTION: In this experiment that we performed, there were many methods that were used to help us manipulate and identify the bacteria E.coli on a MacConkey agar plate. The first part of the experiment involved the methods of manipulating, identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is...

Agar plate, Bacteria, Bacteriology 3401  Words | 9  Pages

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Effects of Different Antibiotics on Bacteria

Effects of Different Antibiotics on Bacteria Because of their small size, rapid rate of reproduction, and relatively simple structure, bacteria are excellent subjects for laboratory studies. This investigation will introduce you to techniques of microbiology that will be of use to you throughout your biological work. Procedure 1. Heat the test tubes of sterile agar medium in the water bath until the agar melts. 2. Remove the test tubes from the water bath. Let them cool enough to hold in your...

Agar, Agar plate, Antibiotic 1420  Words | 4  Pages

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identifying gram positive cocci

IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8, “Identifying Gram Negative Rods”, identifying bacteria is a common activity in the microbiology lab. Like the game Clue™, each time you gather a piece of information to solve the mystery, you gather some information that supports some identities and eliminates others from contention. In the lab, the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities...

Agar plate, Bacteria, Gram staining 2934  Words | 10  Pages

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Isolation of Bacteria

Isolation of Bacteria in our Physical Environment Introduction: The purpose of the lab is to apply what has been learned so far in Microbiology, and use it to collect and examine microbial species from the physical environment. The physical environment around the lab will be utilized to find out the variety of different microbial species growing in and around the lab. This investigation will help in preparation for the individual projects that will be done at the end of the semester. Materials...

Bacteria, Bacterial cell structure, Cotton swab 811  Words | 3  Pages

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Staining: Bacteria and Stain

the differences between the techniques, to able to read results and to have the knowledge for future use in various fields. -Simple stain helps with determining the sizes, shapes and arrangements of microorganisms. -Gram stain assists in identifying the type of microbes, whether they are gram positive or negative, which in turn tells us what kind of cell wall the microbes have. -Endospore stain tells whether microorganisms make endospores. -Acid-fast stain is helpful in recognizing the...

Archaea, Bacteria, Bacteriology 1447  Words | 6  Pages

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Unknow Lab Report

Introduction The purpose of this lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential...

Bacteria, Bacteriology, Gram staining 1319  Words | 4  Pages

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This lab is purposed to familiarize basic equipment and techniques used in the study of microorganisms. In addition, learn some basic techniques used in identifying prokaryotes and make and view microscope slides of some common prokaryotes. In this lab , I worked two experiments , the first one is cultivation bacteria "colony ", and When microorganisms are cultivated in the laboratory, a growth environment called a medium is used , this medium should be sterile ,When grown and...

Archaea, Bacteria, Bacterial cell structure 639  Words | 9  Pages

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Unknown Bacteria #9

 Unknown Bacteria #9 Alexis Chard CRN 9131 Th 5:00-9:00 12/05/2013 Introduction In order to treat a disease correctly and efficiently, it is important to first identify the microbe that is causing the disease. Isolating the bacteria that is causing the disease and using an antibiotic that specifically targets that microbe, can help prevent or reduce the overuse of broad-spectrum antibiotics-which can lead to antibiotic resistance. When trying to determine the type...

Antibiotic resistance, Bacteria, Enterobacteriaceae 1288  Words | 6  Pages

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Systematic Identification of Bacillus Subtilis and Serratia Marcescens Through a Battery of Tests and Plates Introduction

Serratia marcescens Through a Battery of Tests and Plates Introduction: The purpose of this experiment was to use a systematic battery of tube tests and plates designed to lead to identification of two unknown bacterial species, from the combination of all results. A sample of bacteria was used, labeled “Sample 4”, from which both species was to be obtained, one gram positive and one gram negative. Table 1 is a list of the possible bacteria to be identified; the basic ideas and practice of identification...

Agar plate, Bacillus, Bacteria 2767  Words | 8  Pages

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Growing Fungi and Bacteria of Plants

Introduction There are both virulent and non-virulent bacteria and fungi that grow on plants. It is difficult to distinguish between the two without proper inspection and diagnosis of the diseased plant to know whether the bacteria or the fungus in question is the virulent or non-virulent one. Therefore pure cultures need to be isolated to know with absolute certainty which is the causative bacteria or fungus. Potato dextrose agar is a good nutrient agar for mycelia to thrive on which is present in most...

Animal, Bacteria, Cell wall 433  Words | 2  Pages

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Not a paper

 Identifying Micrococcus luteus Identifying Micrococcus luteus Introduction There are many reasons for establishing the identity of a microorganism. The reasons range from the knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. Laboratory scientists are able to isolate, identify, and determine the antimicrobial...

Agar plate, Bacteria, Bacterial cell structure 1186  Words | 4  Pages

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Bacteria Transformation in Biotechnology

Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media, it could be seen that the...

Bacteria, DNA, Escherichia coli 1671  Words | 5  Pages

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Unknown Bacteria Lab Report

as follows. METHODS The two streak plates made from the mixture of unknown bacteria showed two different colony morphologies. Colonies of Bacteria A were translucent in color, circular in form, with entire margins and a smooth surface. Colonies of Bacteria B were cream colored, irregular in form, with undulate margins and an umbonate surface. Two different morphologies allowed Gram stains to be performed on each of the bacterium. Gram staining of Bacteria A revealed pink, rod-shaped cells signifying...

Bacillus, Bacteria, Bacteriology 559  Words | 3  Pages

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Bacteria Colony Appearance Morphology

colonies are rough or smooth in texture. For colony appearances of E. coli and S. aureus, scientists often describe what they look like on agar. This is not the microscopic view (for example, as with a slide) but a “naked eye” view of how the bacterial colonies look while growing on a medium. (This is one type of culture.) If it is just plain nutrient agar (like below) … and it depends on what kind of growth medium is used, the colors should be similar to the pictures below. (In these examples,...

Antibiotic resistance, Bacteria, Escherichia coli 517  Words | 2  Pages

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Bacteria and Gram Stain

Diagnostic Key for A Gram Positive Rods B.subtilis C. xerosis L.casei Starch Plate + - B.subtilis C.xerosis L.casei MR + ...

Bacteria, Enterobacteriaceae, Gram staining 1500  Words | 7  Pages

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Culture Media

major types of growth media: those used for cell culture, which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements...

Agar, Agar plate, Bacteria 1013  Words | 4  Pages

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Diffusion In Agar Cubes

Diffusion In Agar Cubes Is Bigger Better? OR Is Smaller Smarter? Adapted from Flinn Scientific Publication No. 10110 Introduction: Diffusion is one of the very important processes by which substances such as nutrients, water, oxygen, and cellular wasted are transported between living cells and their environment. This activity will help you explore the relationship between diffusion and cell size by experimenting with model “cells.” In this experiment, you will use agar cubes to which...

Cell biology, Chemical kinetics, Sodium hydroxide 826  Words | 4  Pages

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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to, kill the  bacteria, firmly adhere the smear on to the microscopic slide to prevent washing off during staining, and to allow the sample to readily take up the stain. Reference:  www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without...

Bacteria, Bacterial cell structure, Cell wall 4902  Words | 15  Pages

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Double Unkown Final Project

Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore, University of Kansas, Biology 402, Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus...

Agar plate, Bacteria, Bacteriology 1252  Words | 12  Pages

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A Lab Report on Microbial Growth

37℃ commonly found in the intestinal tract of humans and other mammals (Cappucino and Sherman, 2011).. It is a facultative anaerobe that is not normally pathogenic, but pathogenic strains cause UTI’s and bladder infections (SCCC, 2013). Another bacteria that was observed, was Proteus mirabilis. This microorganism is gram-negative and rod shaped. P. mirabilis is motile and “swarms” towards nutrients such as maltose (Murphy, 2004). It is a mesophile, which lives in an optimal temperature of 37℃...

Bacteria, Escherichia coli, Growth medium 1680  Words | 8  Pages

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Unknown Lab Report

Unknown Lab Report Microbiology Unknown A Sonia Kabra November 26, 2014 Introduction There are numerous reasons for identifying unknown bacteria. Some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. Through out the semester in the laboratory, we are able to encounter some of the few microorganisms that we as humans have come into contact with. With the knowledge gained from the sessions in the laboratory, we can now...

Agar plate, Bacteria, Gram staining 1022  Words | 4  Pages

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