Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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Introduction Paper chromatography is a method using a chromatography paper to identify the many mixtures of one simple color. The word chromatography comes from the Greek words‚ "chroma‚" meaning color‚ and "grafein‚" meaning to write. The process of this experiment is to place a colored dot‚ such as marker ink‚ onto the chromatography paper‚ one centimeter away from the triangular tip. Next dip your chromatography paper into the solvent‚ not allowing it to touch the colored dot. Let it sit for
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Paper chromatography is a useful technique for separating and identifying pigments and other molecules from cell extracts that contain a complex mixture of molecules. The solvent moves up the paper by capillary action‚ which occurs as a result of the attraction of solvent molecules to the paper and the attraction of solvent molecules to one another. As the solvent moves up the paper‚ it carries along any substances dissolved in it. The pigments are carried along at different rates because they
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Chromatography of Photosynthetic Pigments Abstract In this experiment a process of chromatography was used to separate chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta carotene. When these pigments were obtained they were used to measure the wavelengths by way of spectrophotometer of each and the total of all the pigments wavelength. Introduction Photosynthesis is a process by which plants use the sunlight to convert it from light energy into chemical energy. This equation shows us how
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INTRODUCTION Plant pigments consist of four main chemicals. These are Chlorophylls‚ The carotenoids‚ Anthocyanin and Betacaine. The aims of this experiment is to separate the plant pigment into different colours using solvents and column chromatography. The hypothesis of this experiment is that this plant pigment from spinach will contain the chlorophyll pigment or may contain the others too. Chlorophyll: Chlorophyll a is the main component of the plant pigments. This is used for the most
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Experiment 5 Analysis of Plant Pigment Purpose: Chromatography is used for separation. In this case the separation of plant pigments. After placing the plant sample in the column the separation will be easy to see and the samples will be analyzed using VIS spectrophotometry. This is used to isolate and identify the different lipids causing the different pigmentations. Introduction: Lipids are chemically diverse substances found in cells. Lipids are water-insoluble‚ structural components
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Tittle: Separation of leaves pigment. Introduction The photosynthetic pigment is used to absorb light during the process of photosynthesis. There are five pigments usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis
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Lab 11- Plant pigment and Photosynthesis Zelda Salfati December 20th‚ 2012 BIOL 1012Y Partners Lab: Nicole Mintz‚ Bayla Kronman
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Submitted: Jan. 9‚ 2013 Separation of Photosynthetic Pigment by Paper Chromatography I. INTRODUCTION There are different types of components in plant pigments. The most important and abundant chemical pigment found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll b. Chlorophyll a‚ being the main photosynthetic pigment‚ has a primary purpose to convert light energy to chemical energy used by the plant itself. Chlorophyll b absorbs light in a region of the
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