Lab 6: Isolation of Chromosomal DNA Mic 428L/ Section 001 Introduction: In biological research to address and eventually answer a multitude of questions‚ usually involves isolating chromosomal DNA. The purpose in this particular lab was to isolate chromosomal DNA from mutants grown and observed in lab 5 and then digest the DNA using a restriction enzyme. The fragments left from digestion will be ligated and then transformed into a strain of E. Coli DH5αλpir containing the pir gene pi product
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Chapter 13—Chromosomal Rearrangements and Changes in Chromosome Number Reshape Eukaryote Genomes Fill in the Blank |1. |Events that reshape genomes by reorganizing the DNA sequences within one or more chromosomes are known as ____________________. | |Ans: |rearrangements | | |Difficulty: 2
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DIFFERENCES BETWEEN PLASMID AND CHROMOSOMAL DNA IN BACTERIA. Eukaryotes have two or more chromosomes‚ prokaryotes such as bacteria possess a single chromosome composed of double-stranded DNA in a loop. DNA is located in the nucleoid of the cell and is not associated with protein A plasmid is an extra-chromosomal DNA molecule separate from the chromosomal DNA which is capable of replicating independently of the chromosomal DNA. Plasmids usually occur naturally in bacteria. A chromosome
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Chromosomal Disorders You will be assigned a random chromosomal disorder. You will research the disorder and answer the questions below. You should include a sketch or picture of the chromosome affected (where the gene is located) or the show the karyotype of the disorder and how it compares to a “normal” karyotype. 1. When was the syndrome first discovered and by who? J. C. P. Williams first discovered it at the year 1961 2. The human chromosomes are numbered. If applicable‚ identify
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Balao‚ Mike Paul C. MT1311 Chromosomal aberrations are abnormalities in the structure or number of chromosomes and are often responsible for genetic disorders. For more than a century‚ scientists have been fascinated by the study of human chromosomes. It was not until 1956‚ however‚ that it was determined that the actual diploid number of chromosomes in a human cell was forty-six (22 pairs of autosomes and two sex chromosomes make up the human genome). In 1959 two discoveries opened a new era
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this process of synapses the two chromosomes of each homolog pair exchange segments of DNA in a process called crossing over. The gene combinations on a chromosome can be changed. For example‚ suppose one chromatid of a chromosome initially contained genes for blue eyes and brown hair‚ by taking the gene for blue eyes from one of the chromatids of that chromosome’s homolog. Prophase I- During prophase I the two members of each pair become intimately associated along their entire lengths (they
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Meiosis is extremely critical because its job is to check that all species produced by sexual reproduction include the right number of chromosomes we should have. This activity decreases the number of chromosomes in half‚ permitting the sexual reproduction process to prevail. Because meiosis produces cells that eventually becomes reproductive cells‚ this division in chromosome number is critical to the cell cycle. Without this process‚ the combination of two gametes during fertilization would result
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Mitosis and Meiosis Purpose To understand the cell cycle and mitosis and how these two processes function within an organism. To understand meiosis and how this process produces gametes. Mitosis All cells in your body complete a cell cycle in which they grow‚ divide and‚ eventually‚ die. The cell cycle consists of four phases: G1‚ S‚ G2‚ and M (Figure 1). Each phase is associated with a specific cellular function: typically growth‚ synthesis‚ and division. The G1‚ S‚ and G2 phases comprise
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Meiosis BIOL 1111 Introduction Meiosis is the second important kind of nuclear division. It resembles mitosis in many ways but the consequences of meiotic divisions are very different from those of mitotic divisions. While mitotic division may occur in almost any living cell of an organism‚ meiosis occurs only in special cells. In animals‚ meiosis is restricted to cells that form gametes (eggs and sperm). Each species has a characteristic number of chromosomes per somatic cell. Fruit flies
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Interphase: DNA replicates ( S-phase) Prophase: Nucleolus fades and chromatin (replicated DNA and associated proteins) condenses into chromosomes. Each replicated chromosome comprises two chromatids‚ both with the same genetic information. Microtubules of the cytoskeleton‚ responsible for cell shape‚ motility and attachment to other cells during interphase‚ disassemble Pro Metaphase: - nuclear envelope breaks down- no longer a recognizable nucleus. Mitotic spindle fibers elongate from the centrosomes
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