"Enzyme Activity Ia" Essays and Research Papers

  • Enzyme Activity Ia

    Abstract: The objective of the Enzyme activity practical was to test the effects of temperature on an enzymes catalyzed reaction. Hydrogen peroxide was used to cause reaction when involved with different temperature levels. Throughout the experiment the data suggested that finding the optimum value involves testing the enzyme in different temperatures. The ideal temperature was 35 ̊C. Introduction: The purpose of this practical was to observe and understand the effects of changes in temperature...

    Chemical reaction, Enzyme, Measurement 751  Words | 3  Pages

  • Enzyme Activity

    Copy button on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity Pam Campbell Id 0002337 Dr. Murphy Nmezi August 9, 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and...

    Acidosis, Base, Blood 1132  Words | 4  Pages

  • Enzyme Activity

    Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages, and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available, the quicker the reaction will happen except in one test...

    Catalase, Catalysis, Chemical equilibrium 1211  Words | 5  Pages

  • Bio Enzyme Activity

    BIOLOGY IA A Factor That Will Affect Enzyme Activity The Question Effects on Enzyme activity: investigating the effects of mixing Hydrogen Peroxide with Catalase extracted from potatoes at different temperatures, and then comparing the results with the same method, except to replace the Potato juice with MnO2 Hypothesis Since enzymes are a type of protein, they will be destroyed at high temperatures and activity rates will go down at low temperatures. Also, if done at low temperature...

    Absolute zero, Chemical reaction, Enzyme 960  Words | 4  Pages

  • Ib Biology Enzymes Ia

    Biology Enzymes IA Design Introduction: Enzymes are globular proteins, they are responsible for most of the chemical activities of a living organism. They act as catalysts, substances that affects the reaction of other substances without being destroyed or altered during the process. They are extremely efficient in the body system of living organisms, one enzyme may catalyse over a thousand chemical reactions every second. But there are certain conditions that need to be fulfilled in order for...

    Catalase, Catalysis, Enzyme 1408  Words | 6  Pages

  • Effect on Enzyme Activity

    EFFECT OF TEMPERATURE,PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature,Ph and substrate concentration on the rate of enzyme activity. Hypothesis ...

    Active site, Catalase, Enzyme 1133  Words | 5  Pages

  • Factors that affect enzyme activity

    8/12/2013 Intro I am going to investigate a factor that affects the rate of enzyme activity What are enzymes and how do they work? Enzymes are biological catalysts - catalysts are substances that increase the rate of chemical reactions without being used up. Enzymes are also proteins and thus have a specific shape. They are therefore specific in the reactions that they catalyse - one enzyme will react with molecules of one substrate. The site of the reaction occurs in an area on...

    Catalase, Catalysis, Chemical reaction 1355  Words | 4  Pages

  • Effect of Temperature on Enzyme Activity

    Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and some...

    Amylase, Catalysis, Energy 1023  Words | 4  Pages

  • Affect of Temp on Enzyme Activity

    The Effect of Temperature on Amylase Activity Truth Teller, Yahoo! Contributor Network Sep 25, 2005 "Share your voice on Yahoo! websites. Start Here." MORE:Enzymes tweet Print Flag Abstract: This study of the effect of temperature on the enzyme amylase was performed to determine the correlation between the two. The experiment was developed to test the enzymes reaction rate of amylase digesting starch at several different temperatures and see how the rate changed. The rate of reaction...

    Amylase, Chemical reaction, Enzyme 1245  Words | 4  Pages

  • Factors Influencing Enzyme Activity

    Abstract In this laboratory exercise we studied enzyme catalase, which accelerates the breakdown of hydrogen peroxide into water and oxygen. The purpose was to isolate catalase and measure the rate of activity under different conditions. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. Changes in temperature and pH along with Substrate Concentration and Enzyme Concentration were the conditions tested in the experiment...

    Catalase, Catalysis, Chemical reaction 1808  Words | 5  Pages

  • Effect of Temperature on Enzyme Activity

    the Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation, you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed reaction? Materials ...

    Catalase, Catalysis, Chemical reaction 1031  Words | 4  Pages

  • Effect of Temperature on Enzyme Activity

    of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature, the rate of amylase activity is very low. ii) At optimum (=best) temperature, the rate of amylase activity is the highest. ...

    Amylase, Amylose, Energy 476  Words | 3  Pages

  • Effect Of PH On Enzyme Activity

    pH on Enzyme Activity Athenaa Jeyachandran Mr.Menes Course Code January 21st, 2013 The Effect on Enzyme Activity Due to Temperatures Purpose: The purpose of this lab experiment is to examine how different temperatures affect enzyme activity using hydrogen peroxide. Hypothesis: My hypothesis for this experiment is that temperatures near body temperature is when enzyme activity will be at its highest. I believe this will occur because in our body, enzymes are very...

    Absolute zero, Anders Celsius, Boiling point 544  Words | 3  Pages

  • Temperature Effects in Enzyme Activity

    Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Without the presence of enzymes some of life's processes would not come so easily. Enzymes are basically proteins, which have specific shapes for different substrates. Enzymes change the rate in chemical reactions. It does this without having to change its own shape, which makes enzymes different from other proteins. A common enzyme that we have is catalase, which breaks down...

    Catalase, Catalysis, Chemical reaction 809  Words | 3  Pages

  • Effect of temperature on enzyme activity

    Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL, 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start...

    Celsius, Enzyme, Fahrenheit 529  Words | 3  Pages

  • Factors affecting the rate of Enzyme activity

    Factors Affecting the Rate of Enzyme Activity The Purpose of doing this experiment is “how factors affect on the rate of enzyme activity.” “We examine the affects of enzyme activity when exposing enzymes to different substrates, PH and temperature. In this experiment a type of enzyme called Peroxides has been used. Peroxidase is the enzymes are found in living organism so, it can be founded in both liver and potato. For measuring the rate of enzyme activity in each experiment the height...

    Catalase, Enzyme, Glucose 1642  Words | 5  Pages

  • The Effect of Tempature on Enzyme Activity

     The Effect of Temperature on Enzyme Activity and Oxygen Production Throughout this report you will gain information as to how temperature effects the amount of oxygen produced in an enzyme- catalase experiment. In the experiment we used liver extract as a catalase and created a chemical reaction within a reaction chamber between the catalase and hydrogen peroxide as well as three different controlled temperatures. In the procedure below there will be a step by step process...

    Catalase, Chemical reaction, Chemistry 1649  Words | 8  Pages

  • enzyme

    Enzyme Lab Name ___________________________ Assignment 1: Getting to Know Enzyme Lab: Setting Up an Experiment The first screen that appears in Enzyme Lab shows you a biochemistry lab containing all the reagents and equipment you will need to perform your experiments. Click on each item in the lab to learn more about its purpose. Once you are familiar with the lab, click on the Experiment button to begin the first assignment. This assignment is designed to help you become...

    Buffer solution, Competitive inhibition, Enzyme 2347  Words | 6  Pages

  • Enzymes

    Topic: Enzymes and their Industrial importance Table of contents Definition of enzyme……….……………………..3 Composition of enzymes…………………………..3 Characteristics of enzymes………………………..3 Functions……..……………………………………..3 Discovery………..…………………………………...5 Explanation…………………………………………..6 Types of enzymes...............................................…...6 Working………………………………………………6 Sources of enzymes………………………………….7 Industrially important enzymes……………….…10 Some other uses……………………………………...

    Archaea, Catalysis, Enzyme 1431  Words | 7  Pages

  • Enzymes

    Enzymes Enzymes are special proteins that are found in the cells of living organisms. Enzymes are made up of long chains of amino acids held together by peptide bonds. Enzymes play an important role in performing or controlling a host of chemical reactions that take place in the body. There are various types of enzymes and usually a specific enzyme is responsible for a particular chemical reaction only. While most of them are associated with metabolic processes like digestion and breathing, there...

    Catalysis, Cell, Chemical reaction 831  Words | 3  Pages

  • Title : the Affects of Temperature on Enzymes Activity

    Title : the affects of temperature on enzymes activity 1. The Problem: measuring and recording the digestion of carbohydrates by enzyme amylase 2. Independent variable: water bath temperature 3. Dependent variable: the time taken for the carbohydrates to get digested by amylases 4. Controls: the amounts of the carbohydrates and the enzymes in the reaction 5. Quantitative measurement: Time | Temperature | Enzymes concentration | Substrate concentration | Starches Indicators...

    Amylase, Catalysis, Chemical reaction 663  Words | 3  Pages

  • enzymes

    LABORATORY REPORT Activity: Enzyme Activity Name: Angela Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature, amount...

    Celsius, Enzyme, Fructose 1044  Words | 2  Pages

  • Enzymes

    Investigating the effect of pH on amylase activity Aim The aim of the experiment is to determine the effects of different pH and the rate of reaction on fungal amylase and starch. Introduction The enzyme amylase is found in the human body, it catalyses the hydrolosis of internal glycosidic bonds in polysaccharides, the breakdown of starch into sugars. Amylase is present in human saliva, where it initiates the chemical process of digestion. Enzymes work best at an optimum pH of 7 which is...

    Amylase, Enzyme, Glucose 1015  Words | 4  Pages

  • Lab report on enzyme activity.

    INTRODUCTION Enzymes are a protein serving as a catalyst, a chemical agent that changes the rate of the reaction without being consumed by the reaction. Enzymes are proteins made up of long chains of amino acids. These form complex shapes. The enzymes are individuals, like the different players on a ball team, they have different specific structures and jobs. As one ball player may be very tall and one short, the specific different shape of the active site on an enzyme is unique and prepares it...

    Chemical reaction, Enzyme, Enzymes 1062  Words | 4  Pages

  • The effect that temperature has on enzyme activity.

    THE RATE OF ENZYME ACTIVITY. To investigate the effect that temperature has on enzyme activity I am going to use the enzyme amylase, which is used as a biological catalyst to break down starch, which cannot pass through the gut wall due to the size of the molecules, into smaller ones. Amylase is a carbohydrase, which converts starch to simple sugars in the Salivary Glands. Three features of all enzymes are: They are always proteins. They are specific in their actions and each enzyme controls one...

    Amylase, Chemical reaction, Enzyme 1615  Words | 6  Pages

  • Enzymes

    CHAPTER 4: ENZYMES Enzymes are biological catalysts. There are about 40,000 different enzymes in human cells, each controlling a different chemical reaction. They increase the rate of reactions by a factor of between 106 to 1012 times, allowing the chemical reactions that make life possible to take place at normal temperatures. They were discovered in fermenting yeast in 1900 by Buchner, and the name enzyme means "in yeast". As well as catalysing all the metabolic reactions of cells (such as respiration...

    Activation energy, Catalysis, Chemical reaction 1858  Words | 7  Pages

  • Enzyme

    Examination of the Effects of Inhibitory and Non-Inhibitory Competition, Enzyme-Substrate Concentration, Along with Varying Temperature and pH-Balanced Environments on the Enzyme-Catalyzed Reaction of pNPP Abstract: Introduction: Many of the chemical reactions, which take place in in living things are controlled by enzymes. In such cases, the enzyme is a protein in the cell which lowers the activation energy of a catalyzed reaction, which serves to increase the rate of the reaction. Alkaline...

    Alkaline phosphatase, Base, Chemical reaction 1876  Words | 6  Pages

  • Enzyme

    Aim To investigate the effect of different temperatures on the activity of rennin in milk Introduction Enzymes are globular protein, responsible for most of the chemical activities of living organisms. They are made up of long chains of amino acids containing carbon, hydrogen, oxygen and nitrogen (Gunsch, 2012). The role of enzyme is to act as catalysts, substances that speed up chemical reactions without being chemically altered during the process. The speeding up of chemical reactions is done...

    Catalysis, Cheese, Chemical reaction 1845  Words | 7  Pages

  • Enzymes!!

    Enzyme Catalysis Lab Problem: Before the lab, one should understand: • The general functions and activities of enzymes; • The relationship between the structure and function of enzymes • The concept of initial reaction rates of enzymes; • How the concept of free energy relates to enzyme activity; • That change in temperature, pH, enzyme concentration, and substrate concentration can affect the initial reaction rates of enzyme-catalyzed reactions; and • Catalyst, catalysis, and catalase ...

    Catalase, Catalysis, Chemical reaction 755  Words | 3  Pages

  • Effect of Ph and Temperature on Enzyme Activity in Skeletal Muscle

    Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle, particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions,...

    Adenosine triphosphate, Catalysis, Cellular respiration 1735  Words | 5  Pages

  • Enzymes

    Biology “Enzyme Activities” Introduction: Enzymes have extremely interesting properties that make them little chemical-reaction machines. The purpose of an enzyme in a cell is to allow the cell to carry out chemical reactions very quickly. These reactions allow the cell to build things or take things apart as needed. This is how a cell grows and reproduces. At the most basic level, a cell is really a little bag full of chemical reactions that are made possible by enzymes (Brain). Laboratory...

    Better, Chemical equilibrium, Chemical reaction 572  Words | 4  Pages

  • Investigation on Effects of DOPA pH on Enzyme Activity

    Investigation on Effects of Different pH on Enzyme Activity How does the different pH buffers affect activity of potato enzyme/extract? Introduction: Proteins are polymers that are made up of smaller units/monomers called amino acids. There are 20 different types of amino acids, thus make up many different combinations in types, numbers of amino acids as well as their orders – an explanantion for why there are so many proteins. Every protein, due to various reactions of amino acids to each...

    Acid, Amino acid, Biochemistry 1225  Words | 6  Pages

  • The Effect of Concentration, Ph and Temperature on Enzyme Activity

    Effect of Concentration, pH and Temperature on Enzyme Activity Biology For Majors October 4, 2012 Abstract We examined the reaction an enzyme has when its concentration, pH and temperature are altered. In order to do this, we added different levels of pH into different test tubes with the enzyme (sucrose), and substrate (sucrose), and we then inverted the tube. The higher pH produced more enzyme activity. Temperature effects enzyme activity by decreasing its stability when the temperature...

    Acid dissociation constant, Buffer solution, Catalysis 2011  Words | 7  Pages

  • ENZYMES

    it's lowest to do so. However, in cold solutions the starch will take longer as it will in temperatures beyond 40 degrees. Once it reaches this point, the break down will either take a very long period of time or have no reaction at all as enzymes are denatured at a certain point. Materials: · 4 x test tubes · 5mL Diastase · 5mL Water · 10mL 2% Starch Suspension. · Pipette · 2 x Spotting tiles · Large Beaker filled with water of assigned temperature · Thermometer...

    Amylase, Chemical reaction, Enzyme 1134  Words | 6  Pages

  • The Effect of Substrate Concentration, Enzyme Concentration, Ph and Temperature on Enzyme Activity”

    Title: “The Effect of Substrate Concentration, Enzyme Concentration, pH and Temperature on Enzyme Activity Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea, combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results....

    Acid dissociation constant, Buffer solution, Carbonic acid 1379  Words | 5  Pages

  • Effect of Temperature on Enzyme Catalase Activity in a Potato

    Effect of Temperature ( C ͦ) on Enzyme Catalase Activity in potato Aim: To investigate the Effect of temperature (10, 37, 60) Celsius (C ͦ) on enzyme catalase activity in potato using 2% of hydrogen peroxide (H202) as the substrate measuring the height (cm) of oxygen gas (bubbles) and calculating the volume of oxygen bubbles produced (cm3) Introduction: Enzymes are biological catalysts that speed up metabolic reactions without being affected. They lower the activation energy needed to start...

    Catalase, Energy, Enzyme 1315  Words | 5  Pages

  • The Effect of Temperature and Ph on Α-Amylase Enzyme Activity

    inside of the amylose coil.The amount of blue complex that starch gives with iodine can be measured by using a spectrophotometer. α-amylases are found in saliva, pancreatic juice, human breast milk, serum and certain tissues such as the liver. This enzyme catalyzes the hydrolysis of α (1-4) linkages in starch by breaking it down to maltose and some glucose. As the starch is broken down, the coiled structure of α-amylase is unfolded. Therefore, iodine will no longer be able to form the blue complex...

    Amylase, Chemical reaction, Enzyme 1379  Words | 5  Pages

  • Effects of Changes in Physical Properties on Enzyme Activity

    Changes in Physical Properties on Enzyme Activity By Alex Hoffmann First Individual Lab Report Wednesday 7:30-10:15pm 10/24/12 Meghan Duell Abstract The goal of this lab was to determine the effects of certain physical properties on enzyme activity. Enzyme activity was measured by the height of the bubbles that appeared after the enzyme was added which are proportional to the rate of the reaction when time is constant. The fact that enzyme activity is affected by physical properties...

    Catalysis, Enzyme, Gas 1906  Words | 6  Pages

  • How does temperature affect enzyme activity?

    Enzyme Activity How does temperature affect enzyme activity? In this practical investigation, my aim is to discover how temperature will affect enzyme activity, by looking at the rate of reaction. I predict that the higher the temperature will be, the faster the reactions take place. However, I also think that there will be an optimum temperature, at which the reaction will work at its fastest; if the temperature goes beyond that, the reactions will stop altogether as the enzymes would have...

    Catalysis, Chemical equilibrium, Chemical reaction 922  Words | 3  Pages

  • Effect of an Increasing Substrate Concentration on Enzyme Activity Rate

    on Enzyme Activity Rate Abstract The reaction rate of an enzyme can be affected by many factors, and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature constant. Our findings illustrate that the rate of enzyme activity...

    Buffer solution, Catalysis, Chemical equilibrium 1007  Words | 4  Pages

  • Investigating the changing effects of temperature on the activity of enzymes

    Investigating the effects of changing temperature on the activity of enzymes Background information: Renin is an enzyme that catalyses the coagulation of milk. It is found in the stomach of many animals and is used in making cheeses and junkets. It is found in the gastric juices or gastric mucosa of many mammals, including humans. In the human stomach, particularly those of infants, rennin works to curdle milk so that pepsin, another stomach enzyme, can further breakdown the proteins into absorbable...

    Chemical reaction, Energy, Enzyme 1503  Words | 6  Pages

  • Effect of Temperature, Study and Measure of Enzymes Activity

    Measure of Enzymes Activity Abstract This experiment investigates the effect that temperature has on the rate of activity of enzyme β-galactosidase and also the rate of β-galactosidase activity in different concentration of substrate over time. Ο-nitrophenylgalactoside (ONPG) is used as a substrate for β-galactosidase. A spectrophotometer is used to detect the change in colour of the substrate. Results show that increase in temperature up to 50oC speeds up the rate of enzyme activity and any increase...

    Chemical potential, Chemical reaction, Chemistry 2441  Words | 7  Pages

  • Enzymes Test for Effect of pH on Catalase Activity

    College General Biology I Enzymes, test for effect of pH on catalase activity Purpose The main purpose of this experiment was to learn about enzymes and how to test for the effect of pH on catalase activity and to be able to tell if a reaction is an exergonic or endergonic process. Introduction Enzymes are made from amino acids, which are made from proteins. In order to make an enzyme, hundreds of amino acids are strung together in a very specific and unique order and eventually is folded...

    Acid, Base, Chemical reaction 658  Words | 3  Pages

  • Enzymes

    Experiment 10 Enzymes Purpose: The purpose of this lab is to observe through experimental procedure on how enzymes affect chemical reactions. Introduction: An enzyme is a protein that serves as a biological catalyst. A catalyst is any substance that increases the rate of a chemical reaction, (done by lowering the activation energy of the reaction). In this experiment we are using Hydrogen peroxide (the substrate for this experiment). Peroxidase is a soluble enzyme normally found in the cytoplasm...

    Catalysis, Chemical kinetics, Chemical reaction 865  Words | 3  Pages

  • Enzymes

    May 1, 2013 Enzymes as Drug Targets Enzymes are defined as any of numerous proteins produced in living cells that accelerate or catalyze the metabolic processes of an organism. Enzymes are usually very selective in the molecules that they act upon, called substrates, often reacting with only a single substrate. The substrate binds to the enzyme at a location called the active site just before the reaction catalyzed by the enzyme takes place. Enzymes can speed up chemical reactions by up to...

    Adenosine triphosphate, Catalysis, Chemical reaction 962  Words | 3  Pages

  • Enzymes

    Explain the structure of enzymes and discuss their metabolic reactions. Explain the structure of enzymes and discuss their metabolic reactions. Enzyme, a protein that catalyses a specific reaction. ‘Living cells contain thousands of different enzymes’ (Elliott, p.214), each of which catalyses (that is, accelerates without itself being changed) just one kind of reaction. In some of these reactions, small organic molecules such as amino acids, sugars, nucleotides, and lipids are broken down to...

    Amino acid, Beta sheet, Enzyme 1681  Words | 6  Pages

  • Biology Enzyme Activity Research Paper

    junket is made by adding the enzyme Rennin to lukewarm milk. Rennin. also known as Chymosin or in its commercial form as Rennet, is found in the fourth stomach of cud-chewing animals, and is to be found in particularly high quantities in the fourth stomach of suckling calves. The enzyme curdles the milk by transforming caseinogen into insoluble casein. The human stomach does not contain Chymosin: instead it breaks down the rough curd in milk by using the Pepsin enzyme, which also breaks down the proteins...

    Boiling, Casein, Cheese 1285  Words | 7  Pages

  • Enzymes

    Enzyme as protein Dr.Samina Haq Quantitative and qualitative test for protein and amino acids • 1. 2. 3. 4. 5. 6. Qualitative test Ninhydrin test Biuret test Xanthoproteic test Millons test Hopkins-cole test Nitroprusside test Quantitative test 1. 2. 3. Spectrophotometric assay Protein shows maximum absorbance at 280nm due to presence of tyrosine and tryptophane. Biuret test shows 540nm Lowry test shows 750nm Ninhydrin Test • Amino acid containing a free...

    Acid, Alcohol, Amine 535  Words | 6  Pages

  • Enzyme Activity

    Title: Enzyme Activity Aim: To investigate the activity of the enzyme catalase in liver and potatoes, and to investigate the effect of temperature, surface area, pH and certain chemicals on the activity of catalase. Equipment: Dilute hydrochloric acid solution x 1ml 10 volume hydrogen peroxide x 100ml Copper sulphate solution x 1ml Aluminum nitrate solution x 1ml Zinc nitrate solution x 1ml dilute NaOH solution x 1ml Mortar and pestle x 1 25ml Beaker x 1 Hot plate x1 ...

    Catalase, Chemical reaction, Chlorine 415  Words | 2  Pages

  • Effect of Concentration on Enzyme Activity Lab Report

    Lab Report (Effect of concentration on enzyme activity) Biology Noor Alawadhi 11- KC Introduction: An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of...

    Chemical reaction, Chemistry, Concentration 619  Words | 3  Pages

  • Enzymes: Enzyme and Variable Groups

    structure of the enzyme is mainly dependent on the active site and variable groups. Extreme temperatures or extreme pHs can alter the structure of an enzyme. Enzymes function to lower the activation energy to break the bonds. They achieve this by putting stress and pressure on the bonds or creating a microenvironment for the substrate. Enzymes are regulated by inhibitors or activators and can be inhibited by the products of the reaction, called feedback inhibition. Enzymes are catalytic proteins;...

    Catalysis, Chemical reaction, Enzyme 1032  Words | 3  Pages

  • Effects of Temperature, Ph, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity

    Effects of Temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes, proteins that act as catalysts, are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes, the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol...

    Base, Buffer solution, Catalysis 2348  Words | 7  Pages

  • Biology Digestion Ia

    Biology Digestion IA 13/03/2013 Jason Gao Rationale: Digestion is the chemical breakdown of food into smaller components that are more easily absorbed. Digestion is a form of catabolism: a breakdown of large food molecules to smaller ones. When food enters into stomach, gastric juice starts protein digestion. Gastric juice mainly contains hydrochloric acid and pepsin. The pH value of hydrochloric in the stomach...

    Digestion, Enzyme, Gastric acid 1380  Words | 5  Pages

  • Enzymes

    Enzyme activity The introduction is a statement of the subject and objectives of the experiment and presents your hypothesis. Relevant background information (appropriately referenced) is given in sufficient detail that a person unfamiliar with the topic can understand the nature of the experiment. A good introduction is clear and concise. Carbohydrates and proteins should be explained in terms of their structure and major functions in cells. Your intro should end with a brief overview of the...

    DNA, Hypothesis, Introduction 2119  Words | 6  Pages

  • Enzyme Lab

    Abstract Enzyme catalysis was observed in order to analyze how changes in temperature, pH, enzyme concentration, and substrate concentration affected an enzyme-catalyzed reaction. This experiment analyzed the rate of enzyme-catalyzed reactions and observed the correlation between catalase activity and products formed. It was found out that the rate of an enzyme-catalyzed reaction starts off rapidly, decreases, and levels off or completely stops, and can be further affected by environmental factors...

    Catalysis, Cell, Digestion 1135  Words | 4  Pages

  • Anatomy: Enzymes

    Enzymes are organic catalysts, usually proteins that speed up metabolic reactions. They lower the amount of energy needed for reactions to progress in cells. In enzymatic activity, the molecules at the beginning are called substrates. Lactose metabolism is when lactose is destroyed, maintained or produced. For instance, being lactose intolerance that’s where lactose is destroyed. Metal cofactors in enzyme activity are required to function properly. The Effect of Temperature on Enzymatic Activity: ...

    Catalysis, Enzyme, Glucose 862  Words | 3  Pages

  • Digestion Self-Design Practical: The Effect of pH on Enzyme Activity

    effect of pH on enzyme activity Rationale Amylase is an enzyme involved in the digestion system which catalyses the breakdown of starch into sugars. It is not only present in human saliva but also in the pancreas, where it hydrolyses dietary starch into disaccharides and trisaccharides which are converted by other enzymes to glucose to supply the body with energy. pH has an effect on the activity of all enzymes, including amylase. As the pH level increases, the enzyme activity increases, up until...

    Acid dissociation constant, Buffer solution, Carbonic acid 1394  Words | 5  Pages

  • The Catalase Enzyme

    Purpose: The purpose of the lab is to observe the breakdown of hydrogen peroxide by the liver enzyme catalase. Determine the factors, such as increased concentration, varying temperature, and changes in state, influence how quickly the reaction takes place, and how well the enzymes function. Hypothesis: For Varying Enzyme concentration, for groups A,B,C, and D, if there is an increase in enzyme concentration as a result of the increasing varied number of discs, then there will be an increased...

    Catalase, Chemical reaction, Enzyme 1061  Words | 4  Pages

  • Restriction Enzymes

    Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses, and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus, most virus particles can initiate a successful infection. When the same strain...

    Bacteria, DNA, Enzyme 1252  Words | 4  Pages

  • Enzyme Lab

    Enzyme Activity Lab Hypothesis: 1] Are enzymes reusable? Yes, enzymes are reusable. 2] Which of the following do you think contain catalase? [Manganese dioxide, chicken meat, apple, carrot, potato, or chicken liver] I think that the manganese dioxide, apple, and carrot contain the catalase. 3] Would grinding the materials affect enzyme activity? I think that grinding the materials will increase enzyme activity. 4] Would temperature affect enzyme activity? I think that...

    Acid, Catalase, Chemical reaction 938  Words | 4  Pages

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