• THESIS
    RESEARCH (Rewrite sa Yellow Pad) 1. Different Culture Media in MCR-PRS Laboratory: a. Can be classified according to: a.i. Consistency: a.i.1. Solid Media (contains 2% agar): for colony morphology, pigmentation and hemolysis. a.i.1.a. Nutrient Agar a.i.1.b. Blood Agar a.i.2. Liquid Media...
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  • Bio L
    -negative bacteria have thin peptidoglycan layers between the plasma membrane and an outer membrane. The thin layer does not retain the purple Gram stain. Colony Morphology (LM page 178–79) Observation: Colony Morphology (LM pages 178–79) Table 14.2 Agar Plates Table data will depend on the...
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  • identification of two species
    nitrate reduction to NO 2. Both samples were streak-inoculated onto TSA plates and incubated at 37o C. The purpose of this procedure was two-fold; first, to culture more of the bacteria in case the original sample was destroyed and second, to isolate colonies for observation of colony morphology...
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  • lab4
    , also, label with your initials, date, and “broth to plate”. 2. Hold the loop as described above, flame the loop, and allow it to cool. 3. Pick up the tube of E. coli, remove the cap, and flame the mouth of the tube as described above. 4. Insert the loop into the culture tube (E. coli) and...
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  • Bio Lab Manuel
    morphology. 1. Find your plates from the previous lab and bring them over to your bench space. Start with the LB and TSA plates. 2. Write down what you see on each plate being as specific as possible including the type of media, numbers of colonies, the color and shape of the colonies, and the...
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  • Microbiology
    * What is "culturable"? a cell which is viable * ................ * Obtaining Pure Cultures * Dilution Streaking * Streak cells on plate * All cells in colony derive from single cell * Genetically identical * Dilution in liquid culture...
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  • Ubiquity of Bacteria
    of bacterial colony morphology and the morphology of individual bacteria: In most cases, the predominant class of microorganisms that you will find on your plates will be the bacteria. Bacteria have different colony morphologies and characteristics that are specific to the genus and species of the...
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  • Catalase
    . One should be labeled as “test” and the other as “control”. 2. Place a small drop of normal saline on each area. 3. With a sterilized and cooled inoculating loop, pick up a small amount of the culture from the nutrient agar slant or Petri plate. 4. Emulsify one or two colonies on each...
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  • Prevalence and Molecular Characteristics of Vibrio Cholerae and Escherichia Coli Specific Bacteriophages in Surface Water Samples of Dhaka City
    Dhaka Isolation of Vibrio cholerae from Environmental Surface Water Identification of Vibrio cholerae Colony Morphology of Vibrio cholerae on TTGA plate Serological Test for Vibrio cholerae Identification of E. coli Colony Morphology of E. coli on MacConkey Agar plate Detection and Isolation of...
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  • Research Proposal
    dissolved in phosphate buffer with pH 6.8 5. 200 μl of LPO dissolved in phosphate buffer with pH 6.8 (control) Table 2. Number of colony forming units of E. coli and S. epidermidis in a medium with the LPO-thiocyanate-H2O2 system Treatments E. coli plates S. epidermidis plates 1...
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  • Lab Exam 1 Review
    Plate (you see streaks on the plate) What are we doing? We are trying to get pure culture—colonies containing a single kind of microbe (in this case a single kind of bacteria). This method We streak a sample containing 2 bacterial species over an agar medium that we prepared. Since we only...
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  • Bleeh
    in Iligan City namely Hospital X and Y was made and these samples were then streaked in the petri plates. All 30 samples from Hospital X yielded colonies. These were then subjected to gram-staining to classify bacteria on the basis of their forms, sizes, cellular morphologies, and Gram reactions...
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  • Medical Unknown
    ) Mannitol fermentation on Mannitol Salt agar (MSA) . **Results : Colonies morphology on plate was given were as follows : circular, raised, smooth, opaque, white-yellow pigmens. After knowing that it was Gram positive cocci , a catalase test and coagulase test was done , in addition to different plates...
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  • Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method
    nutrient agar plates after incubated Three isolation techniques were used; streak plate, spread plate and pour plate and the agar plates were then inverted and incubated at 370C for one day. The distributions of colonies were then observed. Observations were recorded. Isolation Techniques...
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  • Isolation Differentiation
    supernatant. Scale bar: 200 µm. (B) cASCs at passage 3. Scale bar: 200 µm. (C) cASCs at passage 4: cASCs morphology is similar to that found in human ASCs. Scale bar: 100 µm. (D) High-density ASCs culture at passage 4. Scale bar: 200 µm. (E) Karyotype of cASC cell lineage after 10 passages, showing an...
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  • -Positive Staphylococcus aureus Isolates Detection of Oxacillin-Susceptible mecA -Positive Staphylococcus aureus Isolates by Use of Chromogenic Medium MRSA ID
    chromogenic medium, but the isolates were never investigated any further (12).The MRSA ID chromogenic medium targets the ␣-glucosidase enzyme of S. aureus in the presence of cefoxitin (4 ␮g/ml), and positive isolates are visualized as green colonies. The MRSA ID chromogenic medium has a sensitivity of...
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  • Agar plates
    ), therefore there can be black­colored deposits present    Shigella Infections. This photograph depicts the colonial morphology displayed by Shigella boydii bacteria cultivated on a Hektoen enteric (HE) agar surface; colonies of S. boydii bacteria grown on HE agar display a raised, green, and...
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  • studyworld34
    and 10 ml) b. Inoculating loop g. Alcohol lamp c. Fermentation tubes h. Sterile water d. Plated EMB i. Nutrient agar e. Colony counter Procedures: 1st Day - Colony Counting 1. Prepare a serial of the water sample 1:10, 1:100 up to 1:1,000,000 using sterile distilled water...
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  • Lab Report
    transfer a small amount of culture with a flame-sterilized inoculating loop to the first quadrant TSA , after that repeat this process with MSA plate, is high concentration of salt selects for members of the genus staphylococcus some organisms may grow, but typically grow very weakly, and then...
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  • Algal Culturing Technique
    . Sarjeant, W. A. S., Lacalli, T., and Gaines, G. 1987. The cysts and skeletal elements of dinoflagellates: Speculations on the ecological causes for their morphology and development. Micropaleontology 33:1–36. Schnepf, E., and Drebes, G. 1993. Anisogamy in the dinoflagellate Noctiluca? Helgolander...
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