sample of original unknown bacteria #57.
Tube agar slant (for reserve stock #57)
Incubator for 25 & 37 degree celcius and refrigerator
Agar streak plates
Sterile pipettes and sterile water
Gas Pak kit
Inculating loop and inoculating stab
Crystal violet, safarin...
how these differences can be used to design tests for each molecule.
d.) Identify macromolecules in solutions using chemical analyses.
e.) Practice recording data, writing up results, and drawing conclusions.
II. Introduction to the Scientific Method
To design an experiment about a particular...
microorganisms to safe levels), or disinfect (remove most microorganisms but not highly resistant ones) are not sufficient to prevent infection.
E. Describe three common forms of growth that you are likely to see in a broth culture.
Pellicle – mass of organisms floating in or on top if broth
The Antibacterial Effect of
Papaya (Carica) Leaves Extract on Staphylococcus epidermidis
Virgen Milagrosa University Foundation
Special Science High School
Martin P. Posadas Avenue, San Carlos City, Pangasinan
Print/study the lab study pages from Blackboard and answer the questions. Relate these pictures to your own data in the lab manual.
2. Study your lab manual.
3. Study this sheet and make sure your lab manual has the correct answers.
Exercise 1—the Microscope
Know the compound microscope cold—PARTS...
Shigella colonies which do not ferment lactose or sucrose and appear red or white in color with no growth to trace growth on the Agar plate will be present.
Triple Sugar-Iron Agar – Presence of Shigella will manifest as a red slant with a yellow butt with no H2S present.
In Brilliant Green Agar, E. coli...
Can viruses be seen with a light microscope?
no- viruses can only be seen with an electron microscope.
Can the genome of a virus contain both RNA and DNA?
no- they only contain one or the other.
Whats a genome?
entirety of an organism's hereditary information.
True or false: Virus genome might...
Tryptic Soy Broth (TSB) plate with a sample from the environment. TSB is rich in nutrients and can support the growth of a number of bacteria or fungi; therefore you will be able to isolate microorganisms from your hands or other parts of the body or from the oral cavity, from your benchtop or from other...
volume of hydrogen peroxide and observing the reaction. The formation of bubbles, oxygen, indicates a positive result. This easy assay, which can be seen with the naked eye, without the aid of instruments, is possible because catalase has a very high specific activity, which produces a detectable response...
infection sites (Kozier, 2008). Ninety percent of the nosocomial infections are caused by bacterial strains of Pseudomonas aeruginosa, Staphylococcus aureus, E. coli, Klebsiella pneumonia, and Proteus species (Jain, et al., 2007). Nosocomial infection is a major public health problem throughout the world (Bhatt...
Oregon Health & Science University Department of Surgery, 3181 SW Sam Jackson
Park Road Portland, OR 97239 USA.
*Corresponding author: E-mail: email@example.com
2 Biotechnology Vol. 3: Plant Biotechnology
the understanding of chronic wound pathophysiology remains
Life: Origin and Discovery
Read Sections 1.1-1.5
Microbiology: the study of organisms too small to be seen with the naked eye
* Microbe- living organism that requires a microscope to be seen (mm-picometers)
* Microorganisms are everywhere.
* our bodies
throughout my thesis work and during preparation of this dissertation. I feel proud to express my heartfelt gratitude to my reverend research supervisor, Dr. S. M. Faruque, Senior Scientist, Molecular Genetics Laboratory, Laboratory Science Division, ICDDR,B, for allowing me to use the facilities and for his...
(H2O2) added to the milk. Specifically, the effects of the activated lactoperoxidase will be examined on Escherichia coli and Staphylococcus epidermidis through colony counting and measurement of zones of inhibition. Knowing the appropriate concentrations required for activation of the lactoperoxidase system...
Table 2. The observation of the colonies on the nutrient agar plates after incubated
Three isolation techniques were used; streak plate, spread plate and pour plate and the agar plates were then inverted and incubated at 370C for one day. The distributions of colonies were then observed. Observations...
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G1 phase (Gap 1)
S phase (Synthesis)
G2 phase (Gap 2)
G1 phase corresponds to the interval between mitosis and initiation
of DNA replication. During G1 phase the cell is metabolically active and
continuously grows but does not replicate its DNA. S or synthesis phase
marks the period...
cm diameter and held firmly in place wvith a metal flange or hoop pushed over the fabric and around the rim of the support. The agar plate carrying the initial colonies is inverted onto the
I No. 473. This work has been supported by grants from the Research Committee, Graduate School, University of Wisconsin...
the rise. Because of their susceptibility to
oxacillin and cefoxitin, it is very difﬁcult to detect them by using routine phenotypic methods. We describe two such isolates that
were detected by chromogenic medium and conﬁrmed by characterization of the mecA gene element.
y deﬁnition, methicillin-resistant...