nitrate reduction to NO 2.
Both samples were streak-inoculated onto TSA plates and incubated at 37o C. The purpose of
this procedure was two-fold; first, to culture more of the bacteria in case the original sample was
destroyed and second, to isolate colonies for observation of colonymorphology...
RESEARCH (Rewrite sa Yellow Pad)
1. Different Culture Media in MCR-PRS Laboratory:
a. Can be classified according to:
a.i.1. Solid Media (contains 2% agar): for colonymorphology, pigmentation and hemolysis.
a.i.1.a. Nutrient Agar
a.i.1.b. Blood Agar
a.i.2. Liquid Media...
, also, label with your initials, date, and “broth to plate”.
2. Hold the loop as described above, flame the loop, and allow it to cool.
3. Pick up the tube of E. coli, remove the cap, and flame the mouth of the tube as described above.
4. Insert the loop into the culture tube (E. coli) and...
of Disc Diffusion Method (Antibacterial test)
In the preparation of inoculum pick 3-5 well isolated colonies and inoculate to BHI tube. Incubate the tube directly in incubator at 37 C for 2-8 hours until light to moderate growth or turbidity occur. Inoculate the Mueller Hinton Agar plate...
1. Find yourplates from the previous lab and bring them over to your bench space. Start with
the LB and TSA plates.
2. Write down what you see on each plate being as specific as possible including the type of
media, numbers of colonies, the color and shape of the colonies, and the...
shigellosis (Black, 2012).
2. To separate a mixed culture of S. aureus, S. epidermidis, and P. aeruginosa into three pure cultures, the following procedure should be used:
a) Make an isolation plate by diluting the mixed culture until the individual organisms become separated or far enough apart on...
* What is "culturable"? a cell which is viable
* Obtaining Pure Cultures
* Dilution Streaking
* Streak cells on plate
* All cells in colony derive from single cell
* Genetically identical
* Dilution in liquid culture...
of bacterial colonymorphology and the morphology of individual bacteria: In most cases, the predominant class of microorganisms that you will find on yourplates will be the bacteria. Bacteria have different colonymorphologies and characteristics that are specific to the genus and species of the...
Dhaka Isolation of Vibrio cholerae from Environmental Surface Water Identification of Vibrio cholerae ColonyMorphology of Vibrio cholerae on TTGA plate Serological Test for Vibrio cholerae Identification of E. coli ColonyMorphology of E. coli on MacConkey Agar plate Detection and Isolation of...
Plate (you see streaks on the plate)
What are we doing?
We are trying to get pure culture—colonies containing a single kind of microbe (in this case a single kind of bacteria).
We streak a sample containing 2 bacterial species over an agar medium that we prepared.
Since we only...
. One should be labeled as “test” and the other as “control”.
2. Place a small drop of normal saline on each area.
3. With a sterilized and cooled inoculating loop, pick up a small amount of the culture from the nutrient agar slant or Petri plate.
4. Emulsify one or two colonies on each...
in Iligan City namely Hospital X and Y was made and these samples were then streaked in the petri plates. All 30 samples from Hospital X yielded colonies. These were then subjected to gram-staining to classify bacteria on the basis of their forms, sizes, cellular morphologies, and Gram reactions...
dissolved in phosphate buffer with pH 6.8
5. 200 μl of LPO dissolved in phosphate buffer with pH 6.8 (control)
Table 2. Number of colony forming units of E. coli and S. epidermidis in a medium with the LPO-thiocyanate-H2O2 system
Treatments E. coli platesS. epidermidisplates
) Mannitol fermentation on Mannitol Salt agar (MSA) .
Coloniesmorphology on plate was given were as follows : circular, raised, smooth, opaque, white-yellow pigmens. After knowing that it was Gram positive cocci , a catalase test and coagulase test was done , in addition to different plates...
nutrient agar plates after incubated
Three isolation techniques were used; streak plate, spread plate and pour plate and the agar plates were then inverted and incubated at 370C for one day. The distributions of colonies were then observed. Observations were recorded.
supernatant. Scale bar: 200 µm. (B) cASCs at passage 3. Scale bar: 200 µm. (C) cASCs at passage 4: cASCs morphology is similar to that found in human ASCs. Scale bar: 100 µm. (D) High-density ASCs culture at passage 4. Scale bar: 200 µm. (E) Karyotype of cASC cell lineage after 10 passages, showing an...
medium, but the isolates were never investigated any further
(12).The MRSA ID chromogenic medium targets the ␣-glucosidase enzyme of S. aureus in the presence of cefoxitin (4 g/ml),
and positive isolates are visualized as green colonies. The MRSA
ID chromogenic medium has a sensitivity of...
), therefore there can be blackcolored deposits present
Shigella Infections. This photograph depicts the colonial morphology displayed by Shigella boydii
bacteria cultivated on a Hektoen enteric (HE) agar surface; colonies of S. boydii bacteria grown
on HE agar display a raised, green, and...
and 10 ml)
b. Inoculating loop g. Alcohol lamp
c. Fermentation tubes h. Sterile water
d. Plated EMB i. Nutrient agar
e. Colony counter
1st Day - Colony Counting
1. Prepare a serial of the water sample 1:10, 1:100 up to 1:1,000,000 using sterile distilled water...
transfer a small amount of culture with a flame-sterilized inoculating loop to the first quadrant TSA , after that repeat this process with MSA plate, is high concentration of salt selects for members of the genus staphylococcus some organisms may grow, but typically grow very weakly, and then...