and brought it into focus on low power. I saw a lot of tiny particles that appeared to be blood cells through the microscope.
Compare your observations from the fresh yogurt slide and the prepared yogurt slide. Are there differences? Why or why not? The fresh yogurt appeared to have less...
chemicals used to formulate the medium is known.
B. Why is it necessary to use a solid agar medium to obtain a pure culture of S. epidermidis?
To obtain a pure culture, it is necessary to separate individual cells of a particular microbe. This
requires the use of a solid medium that...
Name of media used: Tryptic Soy Agar
2. Media forms used: Agar plate, agar slant, and broth
3. Temperature of incubation: 37 degrees Celsius
List 5 different aseptic techniques utilized to prevent contamination of your culture.
In-text citations required with page numbers for each example...
objectives, focus on one objective, or emphasize a topic that is not in the lab core but is important to that particular course.
d. extrapolating plate counts to obtain correct CFU or PFU in the starting sample 6. Use standard microbiology laboratory equipment correctly, including a. using the standard...
Labset One Worksheet
1. Label and describe the function of each of the following parts of the microscope (7)
A. The ocular lens/eyepiece transmits and magnifies the image from the objective lens
to the eye.
B. The body tube transmits the image from the objective lens to the ocular lens.
construct a scheme to apply to pure colonies isolated by T-streaking on primary and secondary trypticase-soy agar plates. The morphology of the colonies was noted, and bacteria from isolated colonies were Gram stained and cultured on TSA slants. Gram morphology was the basis upon which it was decided...
Friedrich, E. Ininns, K. Moloznik, A. Bandekar, and R. Wrightsman
Table of Contents
Table of Contents 2
Laboratory Safety 4
Map of the Laboratory
Setting Up Your Laboratory Notebook 11
Cleaning Microscope Slides 14
Care of the Microscope
Pouring Agar Plates 22
an important part of the course structure of the university. This is the time students appreciate the theoretical work done in the lecture halls when seen practically. This attachment introduces the student to the outside world in terms of working experience and also prepares them for the future.
Staphylococcus epidermidis infection from the hip replacement surgery or Escherichia coli infection which could be form the abattoir were both patients work. Both infections require different treatment, sample A and Sample B have been sent to the pathology lab to be analysed.
The infection E. coli (Escherichia...
or smoke in the lab. Avoid rubbing your eyes.
2. Shoes with closed toes must be worn in the lab.
3. Extra clothing, books, and other personal items must not be piled on the lab bench. Place these items under the bench or on the racks provided.
4. Wash your work area before you begin the experiments...
if mastered correctly, will make your life and learning experience in Microbiology much easier, if you don’t pay attention and practice these techniques incorrectly, well then……? Today you will be learning the following techniques:
1. Streak plate method for colony isolation
2. Aseptic transfer techniques
cereus, Clostridium perfringens, Corynebacterium xerosis, Enterococcus faecalis, Streptococcus agalactaie, Staphylococcus aureus, and Staphylococcus epidermidis. The Gram negative possibilities are to be narrowed down from Enterobacter aerogenes, Escherichia coli, Moraxella catarrhalis, Neisseria flavescens...
aiding in the cleanup of polluted soils, as well as assist in the decomposition of human waste (Dhamani, 2001).
Since discovered in the late 1600’s by Anton Von Leeuwenhoek, bacteria in the environment have become a main focus in many studies (Black, 2012). It was once believed that bacteria only...
logy= study of or science
is the study of microscopic organisms, either unicellular (single cell), multicellular (cell colony), or acellular (lacking cells).
- 1-2 microns ( 1mm = 1000 microns)
- address them by their proper names ( ie, not "germs" or...
Staphylococcus epidermidis) living on publicly used door knobs
Germs are a mist in every corner of the world. They are stealth and literally, are on everything. This can range from your cooking ware, or...
proceeded as follows.
The two streak plates made from the mixture of unknown bacteria showed two different colonymorphologies. Colonies of Bacteria A were translucent in color, circular in form, with entire margins and a smooth surface. Colonies of Bacteria B were cream colored, irregular in...
Record pH, glucose, blood, protein, ketone within 1-2 minutes
2. Streak plate > 0.001mL sterile loop for HBA (1 vertical + horizontal streak) + normal streak for MAC > Incubate 37°C 24H
3. Antibacterial activity > 1-2 drops of E. coli culture onto MHA > place a sterile disc of filter paper >...
Lab12-Medical Microbiology- Part1– Differential Media/Biochemical Tests, Sp2012
(Set all of your margins to ½”)
Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help...
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