Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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Introduction When plant and animal cells are placed in a hypotonic environment‚ osmosis will occur. The structure of these cells determines the response to the difference in gradient‚ whether this be lysis (the explosion of cells due to the sudden increase in water pressure within the cell) in animal cells or turgor pressure (the pressure created by the increase in water pressure within the cell) in plant cells. Turgor pressure prevents further osmosis‚ which causes the water potential outside the
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Are there any differences in the rate of metabolism of a dried yeast culture with differing carbohydrate sources? In the current practical that was undertaken the growth rate of yeast (S. cerevisiae) with differing carbohydrates sources : Glucose (C6H12O6)‚ Fructose (C6H12O6)‚ Lactose (C12H22O11)‚ Xylitol “(CHOH)3(CH2OH)2” and Water (H2O) as a Control were observed. “ Yeast are single-celled fungi which consist of more than one thousand different species which have been identified. The most commonly
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Introduction: Coccinellidae‚ which is more familiar known as the ladybug‚ comes from a family of small beetles. However‚ one may jump to conclusions after hearing their names‚ but ladybugs are not all females! Their color ranges from a red to a reddish-orange and even to a yellow color along with black spots on their hard-shell wing covers. Even though they are small bugs‚ they are very predatory. The ladybug’s mouth includes mandibles which is another word for their strong jaws (Hussain‚ et al
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Alhia Harris October 18‚ 2011 Biology Lab report Proteins are very important throughout the body. They have a lot of functions in cells. Antibodies are proteins that help cells rebuild the cells when they are destroyed. Transport proteins move substances from one place to another. Regulatory proteins control cellular metabolism in a way that cells throughout the body can have the right amount of glucose in the blood. A common protein is an enzyme; in cells they speed up chemical reactions. For
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The temperature of water was determined by a thermometer. 1 cm stripped ends of the wires was hooked into the input posts on the back of the impendence converter. Lab tutor module was started after. B. Baseline heart rate The top of the container was covered with foil and the animal was allowed to sit quietly for 10 min. The trace was examined by pressing start. After that the baseline or the controlled
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Purpose: The purpose of this lab was to see how the changes in exercise intensity affect the rate of metabolism within the body. Methods: In this lab‚ indirect calorimetry was used to measure metabolic rate by calculating caloric expenditure by the measurements of oxygen consumption. The variables measured were the fraction of oxygen expired‚ the fraction of carbon dioxide expired‚ and the total volume of air inspired. This experiment required the participation of two volunteers. First‚ the variables
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the outmost confidence‚ Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the experiments and comparing them to the other six known bacteria: Micrococcus luteus‚ Staphylococcus aureus‚ Staphylococcus epidermidis‚ Alcaligenes faecalis‚ Escherichia coli‚ and Proteus vulgaris that were used in the lab‚ as well as comparing and contrasting the actual and factual results. Its unique pink rod
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same density. Density also does depend on temperature. For instance cold water is denser than warm water; ice is less dense than both. The method used for determining the density of a substance depends on the nature of the substance. In this lab the densities of unknown irregularly shaped solids and liquids was determined. Accuracy and precision of the results will be estimated‚ and attention will be paid to the correct use of the significant figures. The experiment approach that will be
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1. Coagulase 2. this will differentiate Staphylococcus aureus from Staphylococcus epidermidis. 3. B. Staphylococcus aureus 4. the plasma clotted (gelled)‚ indicating a positive coagulase‚ which indicates S. aureus having followed the diagnostic scheme so far. the beta-hemolysis of this particular strain of S. aureus is not diagnostic of the species. Some strains produce the hemolysin‚ while others don’t. 5. B. Alpha hemolytic and small. (There is a greenish color of the red blood
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