• Rheumatoid Arthritis
    in gene reporter assays. We describe four genotypes which comprise 5, 6, 7, or 8-CATT repeat units and show that the 5-CATT allele has the lowest level of basal and stimulated MIF promoter activity in vitro. The presence of the low expressing, 5-CATT repeat allele correlated with low disease...
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  • Protozoan Diseases
    of diagnosis are developed. These methods include the enzyme-linked immunosorbent assay (ELISA), antigen coated dipsticks, and the direct agglutination test (DAT). Although these tests are readily available, they are not the standard diagnostic tests due to their insufficient sensitivity and...
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  • Oilseed Meals: a Short Review of Prospective Therapeutic Effects
    ) tend to contain the same chemical compounds albeit in different amounts. These compounds can be extracted for use as therapeutic agents and the scientific world of food and medicine have made significant efforts in identifying and elucidating them. In-vitro and vivo assays have proven the potency of...
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  • Trans
    virus followed by subtype H7 specific RTPCR, or by isolation of influenza virus in cell culture and typing of the virus by haemagglutination inhibition assay. Influenza—we defined a probable case of A/H7 influenza as a person who had the opportunity of close contact with HPAI A/H7 (in poultry or...
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  • Immunoglobulin Specificity
    several different species of animals will be analyzed through a series of procedures to determine how well one antibody recognizes identical proteins in those different species. A series of procedures were performed including a Bradford Assay, a serial dilution, an SDS-PAGE, a protein stain...
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  • Titration
     History and etymology * 2 Procedure * 2.1 Preparation techniques * 3 Titration curves * 4 Types of titrations * 4.1 Acid-base titration * 4.2 Redox titration * 4.3 Gas phase titration * 4.4 Complexometric titration * 4.5 Zeta potential titration * 4.6 Assay * 5...
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  • Regulatory Tcells
    accumulated on the skin of the ear and ratios increased when sensitized and challenged when compared to vehicle. In panel F, a Transwell assay was performed to compare Tregs and DCs affinity for CCL21. Results showed that the two types of cells had very similar chemotactic responses. Ear swelling responses...
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  • Isolation and Identification of Pathogenic Bacteria Associated with Frozen Mackerel Fish (Scomber Scombrus) in a Humid Tropical Environment Isolation and Identification of Pathogenic Bacteria Associated with Frozen
    Science Laboratory Technology, University of Nigeria, Nsukka, Nigeria. Accepted 8 January, 2010 Aquaculture products can harbour pathogenic bacteria which are part of the natural micro-flora of the environment. An in-vitro assay was carried out to ascertain and identify major bacterial contaminants...
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  • An exploration of Germ-Line Engineering as a Mechanism for 21st Century Eugenics
    experiments, samples would be taken at set time points to evaluate the effects of time on corrosion. Surface inspection (for color change and cracks), mechanical tests, and chemical assays would be used to determine the extent of corrosion both in vitro and in vivo. Electrochemical testing could...
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  • Biochemical Parameters of Sperm Flagellar Motility Initiation and Regulation
    in vivo (Froman et al., 1999; Bonde et al., 1998). Moreover, in any in vitro fertilization (IVF) program it is a routine practice to select the best sperms by the swimup technique (Akerlof et al., 1991; Mortimer, 1994). The presently available motility assay techniques including the Computer Aided...
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  • Musaddiq
    Chapter 5 DISCUSSIONS The present research work was conducted to evaluate and compare the antioxidant activity of an important vegetable cauliflower of two selected regions of Punjab, Pakistan along with mineral profile analysis as well. The antioxidant activity was calculated by assay TPC, TFC...
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  • Microbiology
    the recombinase XerC and XerD. By using PCR and restriction technology, the vectors for recombination assay were prepared. Using these products, further investigation on the reactions between γ domain and XerD could be conducted and the crystal structure of the four proteins and the fusion proteins...
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  • Research Paper
    . To determine whether the differences in the total antiviral CTL responses were mirrored by viral determinant-specific response, the immunodominant CD8 T cell determinant peptide (TSYKFESV) of VACV/ECTV was used to pulse targets in a CTL assay. This determinant B8R20–27 (encoded by the open reading...
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  • Research (Tobacco)
    intestinal parasites and worms. Thirdly, Nicotine is also an efficient insecticide and can be used as a spray on vegetable.The brine shrimp lethality assay is considered a useful tool for preliminary assessment of toxicity. It has also been suggested for screening pharmacological activities in plant...
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  • Synthesis and Biological Evaluation of Some New Pyrimidines Via a Novel Chalcone Series
    activities of the synthesized compounds (2a-j & 3a-j) for tuberculosis inhibition against the Mycobacterium tuberculosis H37Rv (ATCC27294) strain were performed using the micro plate alamar blue assay (MABA)24 method at TAACF. Compounds exhibiting fluorescence are tested in a BACTEC-460 radiometric...
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  • Proposal Bacterial Biosensors for Victuals Processing
    research are to develop : (1) specific, high affinity biorecognition reagents for food-borne pathogens and toxins, (2) rapid and effective means to separate and concentrate targeted pathogens without carryover of background organisms (3) integrated assay systems based on multiple target biosensor...
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  • Vitamin Identify Machine
    dairy products, is essential for proper growth and for healthy cells. The new approach from ARS scientists is faster than the most commonly used laboratory assay. And, the new technique can be used to individually detect all the various forms of vitamin B12, called cobalamins. The improved assay...
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  • Eugenol
    of eugenol to replicating cells, as mediated by the intracellular level of glutathione and by metabolic activation, was evaluated with the neutral red assay. The cytotoxicity of eugenol to human HFF fibroblasts and human HepG2 hepatoma cells was increased somewhat in the presence of a hepatic S9...
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  • Measurement and Signi®Cance of Circulating
    an unrestricted grant from Biosite Diagnostics, San Diego, California. Manuscript received April 27, 2000; revised manuscript received September 20, 2000; accepted October 26, 2000. Using a recently developed rapid BNP assay (Biosite Diagnostics, San Diego, California), we asked whether BNP levels...
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  • Plant education
    bioautography for antimicrobial assay, antioxidative profiling with DPPH radical solution, acetyl cholinesterase inhibition) on thin layer chromatography (TLC). These plant pre-treatment methods, 38 extraction and analyses were employed in this chapter to determine qualitatively the...
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