Obtain agar plates from your instructor and label them as in the figure 1 provided below. 2. Using a sterile pipet‚ remove 130µL mixed bacterial suspension from the tube “C “‚ remove the lid from the control plate and dispense the bacteria unto the agar. Use a cell spreader to spread the bacteria evenly onto the agar surface. Use of the cell spreader; Dip the cell spreader into ethanol. Pass the spreader
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MacConkey agar plate. The first part of the experiment involved the methods of manipulating‚ identifying and counting the bacteria and the second part was to find out whether the bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the
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Floyd and Dr. Kennedy (1) unless otherwise noted. The first step that was used was a three streak method‚ described in Lab 4‚ on a Blood Heart Infused/Trypticase Soy Agar plate to isolate the two unknown bacteria. Once the two bacteria were incubated‚ grown‚ and isolated they were each individually streaked on a Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and
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quantitative Ames test is similar to the spot-overlay Ames test‚ except that the mutant Salmonella tymphimuium (which cannot composite histidine) is treated with various concentrations of the mutagen Diet Coke to view which sections of the Davis Minimal Agar Plate form colonies (Bjeldanes‚ et al. 1982). Mutagenicity has been known to be carcinogenic and that can cause a toxicological effect that presents a potential health risk for humans ( Resende‚ et al. 2012). The purpose for doing this experiment
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the shape and gram stain of the bacteria pictured. Think on this one! Name the gram stain and shape of the bacteria pictured. Name the genus of the organism pictured. Note the size when determining the organism. Identify this type of agar. We use this agar to check for _______. Name This Technique. What is its purpose? What type of hemolysis is pictured below? Identify the Following I go straight from the 4x objective to the 100 x objective? Why? Identify the Following Identify the
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are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants also contains a pH indicator‚ phenol red‚ which can be used to test the presence of fermentation. If there is a
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Went’s experiment but the agar block was placed on one side of the plant and not in the middle. This caused the plant to grow curved. This is because the auxin went all to one side causing it to grow. One side growing faster then another causes the plant to bend. Gravity can cause an unequal amount of auxin. When a tip of a coleoptile is put on an agar block evenly both side of the block will get equal amounts of auxin but if you were to tip the 90 degrees one side of the agar block would receive more
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The unusual members of NFGNB represent a challenge for the routine clinical microbiological laboratories‚ owing to their complex identification along with their unpredictable antimicrobial susceptibility patterns 14. The isolation rate of NFGNB (18.7%) recorded in our study comes in agreement with a previous Indian study carried out in a tertiary care hospital‚ where it was 16.18% 15. Whereas‚ it comes higher the result recorded in another Indian tertiary care hospital where it was 9.32% 16. The
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the streak‚ meaning that the organism could not use the starch and therefore no starch utilization. The Pseudomonas-F agar was viewed in the dark under UV light and fluoresced a yellow pigment‚ meaning production of pyoverdin and a positive on fluorescent pigmentation. The organism was capable of lipase production as the streak had a clear zone around it in the spirit blue agar‚ meaning the lipase reagent in the medium was degraded and utilized. The gelatinase test showed no clearing on the strip
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Responsibility in Rural Development Sector: Evidences From India A. Manager (Sociology) RITES LTD. (A Government of India Enterprise) RITES Bhawan 1‚ Sector-29‚ Gurgaon-122 001 email: dr_sanjaypradhan@yahoo.co.in‚ akhileshranjan@rediffmail.com Sanjay Pradhan‚ Akhilesh Ranjan Abstract The paper attempts to explore Corporate Social Responsibility (CSR) practices particularly in the context of rural development. The research questions examine do the corporate consider rural people as a stakeholder? If
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