performed in order to discover the activity of the enzyme catecholase in different pH levels as well as its absorbance in differently concentrated solutions. A spetrophotometer was used to measure the absorbance of the enzyme catecholase in different pH solutions as well as to measure the absorbance of catecholase in solutions with different concentrations of potato juice and phosphate buffers. Absorbance of the enzyme catecholase was at an optimum level when pH was close to neutral. When pH was acidic
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than three minutes helps calculate the absorbance which can then be analyzed to find the pseudo rate constant and eventually leading to the rate constant and the rate law. The results show that the m and n are both 1st order which makes the overall order of the blue dye 2nd order. Results: In this experiment‚ rate laws and graphs help calculate the oxidation of food dyes by sodium hypochlorite. During the experiment the linear relationship between absorbance
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measuring the decrease in absorbance at the wavelength corresponding to the absorption maximum(λmax). The hydrolysis is carried out by using a large excess of NaOH (more than 10-fold) with respect to the substratecrystal violet such that the reaction (CV+ + OH-↔ CVOH) obey the pseudo-first order reaction rate equation -d[CV+]/dt = kw[CV+][OH-]=k[CV+] Where k=kw[OH-] is the pseudo-first order rate constant and kw is the second order rate constant. Assuming the absorbance at the λmax of the reaction
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concentration of potassium permanganate (KMnO4) solution by finding its absorbance through the use of spectrophotometer. The preparation of four known concentration of KMnO4 was done namely‚ 2.00×10-4M‚ 1.50×10-4M‚ 1.00×10-4M‚ 5.00×10-5M‚ respectively and is to be place on the spectrophotometer with the unknown and distilled water for the determination of each concentration’s absorbance. As the concentration is proportional with the absorbance of the solution‚ to determine the concentration of the solution
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Results: Part 1: Determination of Amax of bromophenol blue The wavelength with maximum absorbance reading form the graph is 590nm. Part 2: The effect of concentration on absorbance of bromophenol blue solution Tube 1 2 3 4 5 6 Distilled water (ml) 2.5 2.0 1.5 1.0 0.5 0.0 Bromophenol blue 10 mg/L (ml) 0.0 0.5 1.0 1.5 2.0 2.5 Concentration of bromophenol blue (mg/L) 0.0 2.0 4.0 6.0 8.0 10.0 Absorbance at 590 nm (Amax of bromophenol blue) 0.000 0.135 0.199 0.404 0.596 0.724 From Figure 2 above
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Introductory Biology 1 Biology 1003 Fall Term 2011 Lab Number: 3 Title: Cell Energetics: Enzyme Role in Biological Reactions Name: Brandon Moore Student Number: 100819124 Lab day and time: Wednesday pm Date: Wednesday November 23‚ 2011 Introduction Enzymes are a key aspect in our everyday life and are a key to sustaining life. They are biological catalysts that help speed up the rate of reactions. They do this by lowering the activation energy of chemical reactions (Biology
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amounts of Cu in the pennies. The composition of a standard penny is 97.5% Zn and 2.5% Cu. In the lab we will be using Beer’s Law (A=elc+b where A is solution absorbance‚ e is a constant called molar absorbency‚ l is the length in cm‚ and c is the concentration). Using Beer’s Law in this lab a colorimeter is used to find the absorbance and from this the concentration of dissolved Cu2+ ions can be found and percent mass calculated. The techniques used in this lab are useful in that they provide
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Determining the activation energy in the reaction between crystal violet and sodium hydroxide. I. DESIGN ASPECT 1: definition of the problem‚ hypothesis and selection of variables Problem: How the Arrhenius equation can be used to determine the activation energy in the reaction between crystal violet and sodium hydroxide. Objective: The objective of the experiment is to determine the activation energy. Knowing the rate constant k of reactions between crystal violet and sodium hydroxide at
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The wavelength was set to absorbance of 600 nm‚ which is the max for Cu2+. Ten small test tubes were cleaned and a piece of label tape was placed around the top of each tube. A vertical straight line was drawn on the tape to help align with the mark on the instrument sample holder
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processed by the gamma counter onboard software and counts per minute (CPMs) will have been converted to concentrations. The ELISA data contains absorbances (optical densities) from our ELISA microtitre plate. Methods: For ELISA data‚ the standard normal curve is constructed by taking the Log10 concentration of standard progesterone along x axis and mean absorbance estimated from ELISA data on Y axis. This curve then can be used to determine the concentration values of ELISA (pg/100uL). Initially‚ the
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