The method followed is as described in the laboratory manual. In estimating the concentration of the prepared ovalbumin 0.01ml of the preparation was taken‚ rather than the suggested 0.1ml. This was because at the suggested concentration‚ the absorbance at 280nm was off the scale of the machine. In the purification of ovalbumin procedure 0.25g of powdered ammonium sulphate were added per ml of filtrate. 27ml of filtrate was produced 27 x 0.25 = 6.75g
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Absorbance of Light vs. Concentration of Colored Solution Introduction: Performing this lab showed the importance of light in virtual drug screening because light can be used in a variety of ways to obtain different kinds of information in research. Light can be used to determine the concentration of DNA or protein in solution‚ tag different objects such as cells‚ protein structures‚ or bacteria in order to determine whether there is a large amount of a certain substance‚ and to determine the size
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solution. These are all explained by the Beer-Lambert Law. Absorbance (A) = ɛcl A= Absorbance ɛ = molar absorption coefficient (Depend on unit concentration ) c = concentration of colour solution. l = length of the light path. In here two experiments were carried out to clarify the relationship between concentration and absorbance in different wavelengths. According to the Beer-Lambert Law absorbance is directly proportional to the concentration of the colour
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A wavelength of 525 nanometers is within the range for green which is the complementary color for red. The relation between absorbance and percent transmittance is inversely related and as such when absorbance is low then transmittance is high‚ and vice versa. This is given by the equation: A = 2 - log10 %T. Where A is absorbance and T is Percent Transmittance. Transmittance is the percent of light that travels through the sample. When transmittance is at its lowest‚ the corresponding
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photosynthesis system‚ plants and other photosynthesizing creatures they use light of the sun as energy by absorbing some specific wavelength. A prepared sample of the chloroplast from spinach was used during this experiment. In order to observe the absorbance of chloroplast‚ a sample of the 7 mL chloroplast was prepared by pipetting the 7 mL amount of the chloroplast in to the clean‚ dry cuvette. A spectrophotometer was warmed up from the beginning of the lab to get prepared for the actual testing
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experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature‚ 2°C‚ 50°C‚ and 60°C. The overall result of this experiment proved that this reaction works best at room temperature‚ slows down when the temperature is lowered‚ and does
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determine the concentration of two different solutes‚ bromophenol blue and methyl orange‚ in a mixture. Material and method: Refer to practical manual page 7 Results: Part 1: Determination of Amax of bromophemol blue Table 1.1 Wavelength(nm) Absorbance 470 0.064 500 0.116 530 0.232 560 0.422 590 0.752 620 0.172 650 0.026
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hexahydrate and ethylenediamene are mixed at certain stoichiometric ratios of 0.3‚ 0.4‚ 0.5‚ 0.6‚ 0.7‚ 0.8 and 0.9 to form several complexes. Using the UV-Vis Spectrometer‚ the absorbances are taken at wavelengths 530‚ 545‚ 578‚ 622 and 640 nm due to the emergence of several complex compositions. The values of corrected absorbances and the mole fraction of en (X) are plotted to obtain a curve. From these curves‚ an intersection from the 2 trend lines are determined‚ denoting the values of x‚ needed for
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Tan Zhi Chen 0306521 Hypothesis 1) If a calculated absorbance level is directly related to the concentration of albumin‚ then a higher concentration of albumin will lead to higher absorbance level. Results Table No. | Albumin(cm3) | KCl (mL) | Biuret Reagent (mL) | Absorbance | 1 | 0 | 5 | 3 | 0.049 | 2 | 1 | 4 | 3 | 0.112 | 3 | 2 | 3 | 3 | 0.258 | 4 | 4 | 1 | 3 | 0.39 | 5 | 5 | 0 | 3 | 0.418 | 6
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optimum temperature and pH for the reaction of Barley alpha-amylase and starch. The predicted optimum temperature and pH is 50-60 degrees Celsius and pH of 5-6. To test this hypothesis‚ a starch solution was mixed with the amylase and then tested for absorbance‚ using a spectrophotometer‚ at several different temperatures and pHs. From these experiments it was concluded that the optimum temperature was 55 degrees Celsius and the optimum pH was 5. these results lead to the conclusion that the Barley seed
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