enzymes were more fainted compared the stage 1 enzyme. The 96 well plates were put in spectrometer and absorbance data at 595nm were obtained. In figure 4‚ the corrected absorbance at 595nm (after subtracting the background value of the buffer) was plotted against the BSA standard concentration. The equation from the graph was taken to calculate the protein concentration‚ where corrected absorbance at 595nm was y and protein concentration was x. The protein concentration was divided by its respective
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Determination of Gatifloxacin and Ornidazole in Tablet Dosage Forms by High-Performance Thin-Layer Chromatography 43 A simple and sensitive high-performance thin-layer chromatography method has been developed for the quantitative estimation of gatifloxacin and ornidazole in its combined dosage forms. Gatifloxacin and ornidazole were chromatographed on silica Gel 60 F254 TLC plate using n-butanol: methanol: ammonia (6 M) (8:1:1.5 v/v) as the mobile phase and scanned at 302 nm using a Camag TLC Scanner
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essentially for this experiment is to determine the k value or rate constant along with the x and y stoichiometric coefficients given the concentrations of Crystal Violet and Hydroxide. This can be obtained using a spectrophotometer to determine rate of absorbance then graph the rate find a line of best fit‚ determine our rate constant‚ then finally solve for our x and y in the rate law equation. Beer’s law also known as Beer-Lambert law relates the absorption of light to the properties of the material through
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and slightly basic pH environment. This finding partially supports our hypothesis. The spectrophotometer readings in our experiment measured the absorbance of 3-amino-5-nitrosalicylic acid‚ a colored molecule formed after dinitrosalicylic acid (DNSA) has reacted with the products of the enzymatic reaction or the simple sugars. Therefore‚ the absorbance readings correspond to the concentration of the simple sugars produced by the reaction. In the end‚ the concentration of simple sugars indicated the
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Why does belatin leak out of cooked Beetroot? In cooked beetroot‚ the water or food would become stained due to its leakage of betalin (the redish-purple pigment). Betalin is found in the vacuole of beetroot cell. The leakage may have been caused by something happening in the cell membrane of the beetroot – concerning the proteins embedded in the phospholipid layer. The study will require the experiment of different temperatures of water and to find out which in temperature causes the most leakage
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the effect of temperature on the permeability of the plasma membrane of beetroot tissue. Aim- To determine the temperature at which the beetroot plasma membrane is destroyed by heat. Table of results: Temperature of water bath(degrees Celsius) Absorbance(au) (+ or – 0.06au) Repeat 1 Repeat 2 Repeat 3 Mean of results. 30 0.5869 0.6029 0.5946 0.5948 40 0.4161 0.4436 0.7056 0.4298 50 0.4343 0.4077 0.4137 0.4185 60 0.3486 0.4466 0.7991 0.3976 70 1.0500 0.8146 0.8146 1
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The effect of light quantity on photosynthesis of green land plants Introduction Photosynthesis is the process of converting light energy into chemical energy (Hoober 1984). Pigments within chloroplasts‚ primarily chlorophyll‚ absorb the incoming solar energy which excites their electrons (Hoober 1984). These pigments exist in photosystems in the thylakoid membrane of the chloroplast (Ladiges et al.2010). As the electrons return to ground level‚ they are captured by the electron acceptor in the
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Purity of Aspirin Objectives - To research‚ using various sources‚ the history of aspirin‚ its use in medicine‚ methods of synthesizing it and of measuring its purity - To compare the % purity of a branded aspirin tablet with a generic aspirin tablet - To compare 2 methods of composition analysis of the two types of aspirin Research
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the concentration and types of alcohol can affect the ADH reaction. A spectrophotometer is used to determine the concentration of NADH produced in the reaction. Since NADH shows a maximum absorbance at 340 nm that is absent in NAD+‚ the concentration of NADH can be determine by the measurement of the absorbance. It was found that when high concentration of alcohol was used‚ the concentration of NADH will be higher. However the equilibrium constants remain the same in different concentration of alcohol
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PREFORMULATION STUDIES Preformulation may be described as the process of optimizing a drug through determination and identification of those physical and chemical properties considered important in the formulation of a stable‚ effective and safe dosage form. Preformulation studies focus on those physicochemical properties of the compound that could affect drug performance and development of an efficacious dosage form. These Preformulation investigations merely confirm that there are no significant
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