Introduction In this experiment‚ the gram negative bacterium Escherichia coli is being subjected to various environmental factors that affect the rate of growth. These factors scrutinized were the different types of nutrients‚ the intensity of aeration‚ or the temperature at which it was stored. The purpose of this lab is to determine which factor affects the Escherichia coli the greatest. It is known that these abiotic factors affect the rate of growth the greatest if they remain at the correct
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MANGOSTEEN FRUIT | | | | The Mangosteen Fruit is by far the most exotic fruit in the world. It has been researched and studied so much that this site is dedicated to bring you all that information and research. | | The Mangosteen contains several classes of phytonutrient antioxidants and is the only place in nature to contain a broad spectrum of a new class of phytonutrients called Xanthones. The potent effects on human health of the Mangosteen have been observed for centuries in Southeast
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Determining absorbance of various wavelengths of light for pigments present in Coleus plants Joseph Yung (King Yung) 212831426 Adrian Ionescu Section M 11 February 5‚ 2014 Absorbance Table Absorbance Spectra Figure 1: Absorption spectra of pigments found‚ through chromatography‚ within Coleus plants. The different wavelengths of light were determined by the use of a spectrophotometer Questions
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Abstract In this experiment‚ the absorbance of KMnO4 was measured by spectrophotometric method to determine the molar concentration and the molar extinction coefficient of KMnO4. In part 1‚ in order to determine the maximum absorbance wavelength of KMnO4‚ we measured the absorbance of the sample solution which contains KMnO4 at the wavelengths between 330nm and 660nm‚ and plotted the λ and A points; the λmax was 530nm. In part 2‚ the effect of concentration on the absorbance was examined. We prepared five
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Figure 1 shows the average amount of absorbance for each tube‚ containing different levels of pH. Tube 2 had an acidic pH level‚ Tube 3 had a neutral pH level‚ and Tube 4 had a basic pH level. It is indicated that the absorbance rates were the highest for the neutral pH level‚ with a final absorbance rate of 0.166. This was followed by a basic pH (0.106). The acidic pH level had the least amount of absorbance with a final absorbance rate of 0.069. This reinforces the idea that the ALP enzyme worked
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opposed to those with a lower concentration. The absorbance range for the standard curve (figure 1) was determined to be between 0.047A and 0.317A (table 1). This was determined to be the range in which the unknown absorbance had to fall. Unknown C was diluted by a factor of 1 μL UC/1001 μL dH¬¬2O and had an absorbance of .0754A. The resulting solution had a light blue coloration. The dilution was decreased to 1 μL UC/501 μL dH2O and had an absorbance of 0.089A (table 2). This solution had a slightly
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this experiment are tap water mixed with food colouring‚ Introduction: The Beer Lambert Law shows the relation between absorbance of light of an object‚ the molar absorptivity‚ the concentration of the substance‚ and the distance the light travels. The Beer Lambert Law states that there is a linear relationship between the concentration of a solution and the absorbance of said solution. If there is a linear relation‚ the resulting graph should be a straight line. If the graph is a straight line
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5-dichloroaniline which reacts with bilirubin to form azobilirubin with maximum absorbance at 540nm. Procedure (B) : Estimation of Direct Bilirubin. This methods are based on the reaction between direct (conjugated) bilirubin and diazotized sulphanilic acid solutions. Direct Bilirubin reagent utilizes an acid diazo method. Conjugated bilirubin will reacts with diazotized sulphanilic acid to produce an acid azobilirubin‚ the absorbance of which is proportional to the concentration of direct bilirubin in the
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water by spectrophotometry. Abstract: The iron in natural water was determined by utilizing spectrophotometric analysis. That was done by measuring the absorbance of five Fe(oPH)2+3 standards at 510 nm. From that information‚ a calibration curve was plotted and used to find the amount of Fe2+ that was in two unknown water samples based on the absorbance readings obtained with them at 510nm. The equation of the line was found to be y=0.1765x + 0.0705. It was then determined that there was no iron present
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Beer-Lambert Law Relationship Between Molar Concentration and Absorbance Solution colour results from the absorbance of some light wavelengths by solutes dissolved in solution‚ while allowing other wavelengths to pass through (transmittance). The combination of the remaining wavelengths that pass through results in the colour of the solution. A colorimeter can be used to determine the amount of light at a particular wavelength that is absorbed/transmitted by a solution. Depending on the concentration
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