capillary tube‚ leaf pigment extract in acetone‚ beakers‚ forceps‚ scissors‚ acetone‚ petroleum ether and acetone solvent‚ chromatography paper‚ wax paper‚ spectrophotometer‚ test tubes Measurements: Absorption spectrum‚ percent transmittance to absorbance Results: (See graph on page 2) Discussion: My hypothesis for this experiment‚ if chlorophyll a
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100L = 0.007 mol (249.5896g) = 1.747g Concentration (M) Wavelength Determination (Trial One) Wavelength Determination (Trial Two) 0.13M 50%T 49%T 0.11M 55%T 54%T 0.090M 62%T 59%T 0.070M 68%T 67%T Concentration (M) Absorbance Measurements (Trial One) 2-log(%T) Absorbance Measurements (Trial Two) 2-log(%T) 0.13M 0.30M 0.30M 0.11M 0.26M 0.27M 0.090M 0.21M 0.23M 0.070M 0.17M 0.17M Unknown amount of CuSO₄: 52.0%T 2-log (%T) 2-log (52.0) =
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Mustaqim Ahmed Period 4 September 18‚ 2013 Analysis of Food Dyes in Beverages Purpose: To determine the concentration of dye in a sports drink using spectroscopy and graphical analysis. We will be using beers law to find the unknown concentration of dye in a sports drink. Materials: Beakers‚ 50-mL‚ 2-3 ; Test tubes (13 x 100)mm 3-8 ; FD&C Blue 1 stock solution‚ 50-mL ; Lens tissues ; Pipets‚ serological‚ 10-mL ; Sample of consumer sports drink‚ 10-mL ; Spectrophotometer ; Test tube rack Procedure:
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report investigates the absorbance of methylene blue and carmine red using a spectrophotometer to determine the absorption spectra of both solutions. The concentration of the unknown solution of methylene blue was found to be 1.07 x 10-5 M by using the molar extinction coefficient‚ with absorption of 0.547. It was also found that the results concluded confirmed beer’s law with an R2 value of 0.9989. Introduction Spectrophotometry is the quantitative measurement of the absorbance or transmittance properties
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Determination of Trace Amount of Copper and Zinc in an Aqueous Solution by Atomic Absorbance Spectroscopy Abstract: The purpose of this experiment is to analyze a sample containing copper and zinc in trace amounts‚ where standard addition method was used and the instrument used is the atomic absorbance spectroscopy. The amount of copper obtained was 0.569 ppm ± 0.015 and for zinc we obtained 0.42 ppm ± 0.0027. Introduction: This method implies the excitation of particles in the solution sample
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According to the results‚ there was no significant difference between the filtered 100% water composition‚ and the filtered 90% water 10% soap composition. However‚ based on fFigure 2‚ the Average of Absorbance graph‚ showed that growth of the algae for the 100% water composition was observed to be slightly higher than the 90% water 10% soap composition. Meaning the soap may have slightly affected the growth of the algae‚ in some way. One possible‚ reason for a slightly higher growth for the filtered
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involves measuring the absorbance of several concentrations of the pure substance or the "standard" substance determine relationship between concentration and absorbance compared results from unknowns How to use Linear Regression for Generating a Standard Curve? © 2010 by M. Olaveson UTSC 2 BIO A01F-Fall 2010 - ASSIGNMENT # 2 - Preparing a Standard Curve using Excel 2007 Assignment # 2 Analysis of Data from Lab 2-Exercise 2 Table 2.6. Protein in Test Tubes prepared for
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Mass Determination of Ca‚ Fe and Zn in a multivitamin using Atomic Absorbance Spectroscopy (AAS) and X-Ray Fluorescence Spectroscopy (XRF) ABSTRACT: The purpose of this project was to determine the amount of calcium‚ iron and zinc present in an over the counter multivitamin. This mass was determined using atomic absorption spectroscopy (AAS) and X-ray fluorescence spectroscopy (XRF). For both analytical techniques‚ the method of standard additions was used to compensate for matrix effects.
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| Bubble formation was observed as the leafs were being heated. | Table 1.1: Qualitative Data Wavelength of Incident Light (nm) | 400 | 440 | 480 | 520 | 560 | 600 | 640 | 680 | 700 | Relative Absorbance (±0.005nm) Group 1 | 0.09 | 0.11 | 0.04 | 0.01 | 0.01 | 0.01 | 0.03 | 0.03 | 0.01 | Group 2 | 0.14 | 0.15 | 0.07 | 0.02 | 0.02 | 0.02 | 0.04 | 0.04 | 0.01 | Group
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the pH of bromothymol blue affects the absorbance value of the solution which determines the equilibrium constant (pKa) of the indicator. Variables: Variables Variables Measured Method of measurement Independent pH of the six buffer solution A pH probe attached to a data-logger will be used to measure pH Dependent Absorption of the buffer solutions at wavelength 435.0nm and 617.0 nm A spectrophotometer (±0.001) will be used to measure absorbance at wavelength 436.0nm and 617.0 nm respectively
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