Preview

Yeast Fermentation

Powerful Essays
Open Document
Open Document
3511 Words
Grammar
Grammar
Plagiarism
Plagiarism
Writing
Writing
Score
Score
Yeast Fermentation
Title :
Measuring the rate of oxygen uptake.

Objectives : 1. To demonstrate the uptake of oxygen in respiration. 2. To measure the rate at which an organism respires. 3. To learn how to set up the apparatus for respirometers.

Introduction : Respirometer A respirometer is a device that been used to measure the rate of respiration of a living organism. This can be measured by calculating the rate of exchange of oxygen and carbon dioxide. A simple respirometer designed to measure oxygen uptake or CO2 release consists of a sealed container with the living specimen together with a substance to absorb the carbon dioxide given off during respiration, such as soda lime pellets or cotton wads soaked with potassium hydroxide. The oxygen uptake is detected by displacement of coloured liquid in a thin glass U-tube connected to the container. When the organism takes in oxygen it gives off an equal volume of carbon dioxide. The soda lime or KOH will absorbed this carbon dioxide therefore air will be sucked in from the manometer to keep the pressure constant. The rate of changes gives a direct and reasonably accurate reading for the organism's rate of respiration.Whether measuring a whole organism or a sample, a respirometer needs to be sealed to collect valid information. The environment inside the respirometer is tightly controlled and monitored to collect data without causing injury to the organism being studied. These devices must be carefully monitored during use to avoid creating a dangerous situation inside the respirometer. If the space becomes filled with metabolic wastes, the organism may not be able to breathe, and conversely, if the organism uses up substances such as oxygen, it will be in danger. Tight controls of the environment ensure that the pressure in the manometer is under control.

Respiration The term respiration has two relatively distinct meanings in biology. First, respiration is the process by which an organism takes oxygen into

You May Also Find These Documents Helpful

  • Powerful Essays

    Unit 5 P4 M1

    • 1902 Words
    • 6 Pages

    Respiration can happen without oxygen. This process is called anaerobic respiration. The equation glucose+ oxygen which produces energy along with the bi-products produces 38 Adenosine Triphosphate (ATP). It produces more ATP because it has oxygen present (aerobic) this is used more effectively in the body for things like growing. The rest is wasted through heat as a bi-product which can be good as we need to stay warm. ATP is the unit for measuring energy. Respiration without oxygen produces 2 ATP so the energy is not as efficient as there is not as much ATP being produced. Whereas from the equation where oxygen is present, it produces 38 ATP which is more efficient which is used for activities like movement. (Khan Academy, 2009)…

    • 1902 Words
    • 6 Pages
    Powerful Essays
  • Satisfactory Essays

    Respiration is defined as the process of inhalation of oxygen and the exhalation of carbon dioxide with the help of certain organs of the human body. These organs include the nose, pharynx, larynx, trachea, bronchi and the lungs, altogether they make up the human respiratory system which is divided in two parts by the upper and lower respiratory tract.…

    • 347 Words
    • 2 Pages
    Satisfactory Essays
  • Good Essays

    Respiration begins when oxygen enters the body through the nose and mouth. Respiration is the primary function of…

    • 465 Words
    • 5 Pages
    Good Essays
  • Better Essays

    Yeast Pre-Lab

    • 788 Words
    • 4 Pages

    Yeasts’ capability of undergoing ethanol fermentation, its ability to ferment other sugars and artificial sweeteners, and how lactase influences yeasts ability to use lactose as a food source…

    • 788 Words
    • 4 Pages
    Better Essays
  • Good Essays

    Respiration is the process that takes place in every living cell to make energy available to the body. Energy is vital for us to carry out many life processes such as movement and so respiration in essential to life. During respiration glucose and oxygen are used in a reaction that produces energy and gives out carbon dioxide and water as waste products. Glucose is obtained by digestion of food and oxygen is taken from the air during a process called breathing or ventilation. Pulmonary Ventilation is the term given to the movement of air in and out of the lungs; the rate of pulmonary ventilation is defined as the tidal volume multiplied by the number of breaths taken per minute. Tidal volume is the volume of air breathed in or out during one cycle of quiet breathing. There are a few different factors that can affect the pulmonary ventilation rate but the most common and often most noticeable variation in pulmonary variation rate occurs during exercise.…

    • 872 Words
    • 3 Pages
    Good Essays
  • Good Essays

    Respiration is a series of reactions in which energy is released from glucose. Aerobic respiration is the form of respiration which uses oxygen. It can be summarised by this equation: (Reference 3)…

    • 811 Words
    • 4 Pages
    Good Essays
  • Powerful Essays

    Cellular respiration is the process of oxidizing food molecules, like glucose, to carbon dioxide and water.…

    • 1732 Words
    • 7 Pages
    Powerful Essays
  • Good Essays

    Determining Lung Capacity

    • 803 Words
    • 4 Pages

    This is the experiment of determining lung capacity. All the students in the group measured three types of different parameters: vital capacity, expiratory reserve and tidal volume.…

    • 803 Words
    • 4 Pages
    Good Essays
  • Satisfactory Essays

    This double chambered respirometer, allows you to measure the pressure changes, due to the presence of a control tube.…

    • 360 Words
    • 2 Pages
    Satisfactory Essays
  • Better Essays

    Sugars are vital to all living organisms. The eukaryotic fungi, yeast, have the ability to use some, but not all sugars as a food source by metabolizing sugar in two ways, aerobically, with the aid of oxygen, or anaerobically, without oxygen. The decomposition reaction that takes place when yeast breaks down the hydrocarbon molecules is called cell respiration. As the aerobic respiration breaks down glucose to form viable ATP, oxygen gas is consumed and carbon dioxide is produced. This lab focuses on studying the rate of cellular respiration of saccharomyces cerevisiae, baker’s yeast, in an aerobic environment with glucose, sucrose, lactose, artificial sweetener, and water as a negative control. A CO2 Gas Sensor Probe is used to measure the amount carbon dioxide produced as the cellular respiration occurs which is proportional to how much of the molecule is decomposed. For this experiment water is used as a treatment control to provide a baseline for all the other treatments. To ensure the validity of the experiment, the amount of time the yeast was exposed to the sugars, the designated pipets for each sugar, the amount of sugar tested, and the temperature of the yeast culture were monitored to be the same throughout the experiment.…

    • 844 Words
    • 4 Pages
    Better Essays
  • Good Essays

    To design and conduct an experiment to explore other factors, including different environmental variables, on the rate of transpiration…

    • 907 Words
    • 6 Pages
    Good Essays
  • Good Essays

    Respiration is the process of releasing energy from glucose or any other organic material. The chemical energy in glucose is used for growth and movement. But the process of respiration in plants is different to those in animals. In respiration plants use carbon dioxide and burn the sugars they produced from photosynthesis and convert it back to energy.…

    • 582 Words
    • 3 Pages
    Good Essays
  • Better Essays

    Photosynthesis Lab Report

    • 1265 Words
    • 6 Pages

    In experiment one I hypothesize that the effect of respiration changes on carbon dioxide, concentration in exhaled air will be greatest the longer the respiration rate is increased. I predict that during normal breathing the time it takes to detect the carbon dioxide will be greatest.…

    • 1265 Words
    • 6 Pages
    Better Essays
  • Good Essays

    In order to grow, the seeds need a high amount of ATP because they need to sprout and get bigger. Knowing this we hypothesized that if the germinating seeds consume more oxygen than the dry seeds, then the rate of respiration is higher in germinating seeds. This is because germinating seeds were growing and needed oxygen to produce ATP and continue to grow. The purpose of this was to see the difference in respiration rates of non-germinating seeds and germinating seeds.We did this by using respirometers. This is a device that measures the rate of respiration of a living organism by measuring the rate of exchange of oxygen and/or carbon dioxide. It is composed of a vile that contains the peas and air, the vial is sealed with a rubber stopper and a pipet is inserted into the hole. The set up is explained in the methods section. The way it works is as the peas respire, oxygen is used and carbon dioxide is released. It is important to keep the experiment conditions at a constant temperature and pressure. In doing so, according to Avogadro's Law, one mole of carbon dioxide is released for every mole of oxygen. Thus, there will be no change to the volume in the respirometer. To counteract this issue we add KOH. The following reaction occurs: CO2 + 2KOH —> K2CO3 +H2O. The carbon dioxide that is produced is removed because of the reaction with KOH.…

    • 534 Words
    • 3 Pages
    Good Essays
  • Satisfactory Essays

    Yeast Coorperation

    • 264 Words
    • 2 Pages

    Since this genetic conflict occurs as a result of a trade-off between selection for higher vertical transmission which favours higher plasmid copy number and selection for lowering fitness cost to the host which would favour reduced copy number, this genetic conflict would be reduced if the later where of greater value. This could be done by reducing the efficiency of vertical transmission between the plasmids to maximize the rate of plasmid loss during asexual growth.…

    • 264 Words
    • 2 Pages
    Satisfactory Essays

Related Topics