Isolation of bacteria from mechanic workshops’ soil environment contaminated with used engine oil
T. K. C. Udeani*, A .A. Obroh, C .N. Okwuosa, P. U. Achukwu and N. Azubike
Department of Medical Laboratory Sciences, College of Medicine, University of Nigeria, Enugu Campus, Enugu, Nigeria.
Accepted 28 May, 2008
The study was designed to evaluate the bacterial diversity of soil environment contaminated with used engine oil. Ten mechanic workshops within Enugu metropolis were selected and six soil samples were collected from each site. These were analyzed using Bushnell Haas enrichment medium. Samples were enumerated using ten fold dilutions from 1:10 to 1:100000 from the soil samples. The result showed the isolation of Bacillus Stearothermophilus (8.3%) and Cyanobacteria (1.7%) from the sites sampled. The number of viable bacterial growth of B. Stearothermophilus and Cyanobacteria were enumerated and 4 4 5 expressed in colony forming units. Agbani had bacteria densities of 5 x 10 , 1.25 x 10 and 6.25 x 10 4 4 from the three different sites respectively, Asata with 5.10 x 10 and 2.5 x 10 , independence layout with 2 4 5 bacterial density of 2.5 x 10 and Uwani/Coal camp with 2.5 x 10 and 6.25 x 10 . Amongst the 10 5 different sites studied, Agbani and Uwani/Coal camp had the highest bacteria density of 6.25 x 10 . Key words: Used engine oil, contaminated soil, pollution. INTRODUCTION Environmental pollution with petroleum and petroleum products (complex mixture of hydrocarbons) has been recognized as one of the most serious current problems especially when associated with accidental spills on large-scale. If this occurs, hydrocarbons may reach the water table before becoming immobilized in the soil. They spread horizontally on the ground water surface and continue... [continues]
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