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Kamias (Averrhoa bilimbi) LEAVES AND FRUIT EXTRACT:
ITS ANTIBACTERIAL EFFECT ON Escherichia coli, Staphylococcus aureus AND Salmonella enteritidis

A Science Investigatory Project as

an Entry for the 2008 School Level Science Fair

Ellaine M. Andales
Angelica Mae J. Flores Hannah C. Mendoza
Research Proponents

Mrs. Noreen T. Catis Project Adviser

REGIONAL SCIENCE HIGH SCHOOL Malasiga, San Roque, Zamboanga City

October, 2008

ABSTRACT

This research study aims to prevent diseases caused by Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. The study makes use of kamias (Averrhoa bilimbi) leaves and fruit extract as an antibacterial agent that is more or equally effective than the commercialized one and at the same time, available in our environment. Ten Replicates on ten Petri dishes were prepared for each bacteria. Pure cultures of the three bacteria were spread on the replicates. After extracting the needed substances from the kamias leaves and fruit extract, the researchers were able to begin the experimentation. Six treatments were made in the set-up-Treatment A as 100% water served as the negative control; Treatment B as 100% Kamias leaves extract; Treatment C as 50% Distilled water and 50% Kamias leaves extract; Treatment D as 100% antibiotics was used as the positive control because of its known antibacterial properties; Treatment E as 100% Kamas fruit extract and Treatment F as 50% Distilled water and 50% Kamias fruit extract. The disk-diffusion test was admonished to the replicates and the data were recorded after 24 hours of incubation at 35.5 degrees Centigrade of all the treated dishes. The data gathered were subjected to Analysis of Variance (ANOVA) and Scheffe’s Test. Results of statistical analyses show that Treatment B and Treatment E is equally effective with Treatment D for Escherichia coli, Staphylococcus aureus and Salmonella enteritidis. This study led to the conclusion that Kamias leaves and fruit extract is an effective antibacterial against Escherichia coli, Staphylococcus aureus and Salmonella enteritidis.

ACKNOWLEDGMENT

The Researchers would like to acknowledge and express their deepest sincerity to the following that had contributed a lot to the success of this Research Study.

➢ Their beloved and supportive parents who are always ready to support them financially and morally for the completion of this study. ➢ R.S.H.S. principal, Mrs. Melanie A. Minez, their class adviser, Mrs. Collin Ceneciro and the faculty members for their moral support and consideration to the Researchers’ hectic schedule. ➢ The Department of Science and Technology for providing them vital information and the materials needed for the actual experimentation. ➢ Mrs. Jovita Amparo and Ms. Arlene S. Herbieto and the personnel of the Department of Science and Technology for sharing their knowledge concerning our research study and for guiding the researchers to conduct their actual experimentation. ➢ Ms. Felsa Fae C. Mendoza for helping the group to gather more information about the study. ➢ The IV-Zara class for their moral support in accomplishing this research proposal. ➢ Their amiable Research Adviser, Mrs. Noreen T. Catis for her guidance, motivation and for the wisdom he had imparted to us. ➢ And above all, to our Almighty God...
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