Virtual Lab Report: Part I
Due by: 11:59 PM PST on the second Saturday of class
Virtual Lab 1: Virtual Microscopy
A. Estimate the size (length and width) of these microscopic objects in micrometers (microns): 1. An E. Coli cell. 3 x 0.6 um =1.8 um
2 A mitochondrion. 4 x 0.8 um = 3.2 um
3. A Red blood cell. 8 um
4. A virus. _Hepatitis 45 nm = .045 um
5. A water molecule. 275 pm =.275 um
1 Describe three differences between prokaryotic and eukaryotic cells. Prokaryotic don’t have a nucleus and their cell type is unicellular. They do not have a true membrane bound nucleus and they have loop DNA. They are rod shaped, spherical and spiral and they divide by binary fission. They are smaller in size as well. Eukaryotic have tangled DNA and they have a nucleus. They have a multicellular cell type and they have a true membrane bound nucleus and they depend on oxygen for their metabolism. They are larger in size.
All of this information was found via the web on this url: http://www.diffen.com/difference/Eukaryotic_Cell_vs_Prokaryotic_Cell
2. Describe three differences and three similarities between plant and animal cells. Three similarities: Both have a cytoplasm, ribosomes, mitochondria and a nucleus. Three differences: The plant cell has a cell wall and the animal cell does not. The animal cell is round and the plant cell is rectangular.
The plant cell has plastids and the animal cell does not.
All of this information was found via the web on this url: http://www.diffen.com/difference/Animal_Cell_vs_Plant_Cell
C. Form a hypothesis
1. Hypothesize about how you might be able to sort a mixed population of cells into prokaryotes and eukaryotes. Try to be practical, build on your understanding of the differences between the two cell classes. My best idea would be to sort them based on size since the eukaryote cells are smaller in size and the prokaryote cells are larger in size and shape. Another way to tell is that the eukaryote has a nucleus and will have a large amount of organelles that are visible through the microscope.
Here is the video that helped me understand this: http://www.youtube.com/watch?v=yWy4o_UfZ4A&feature=player_embedded#!
2. Hypothesize about a means to separate out plant cells from a mixed sample of eukaryotic cells. Plant cells have a cell wall to give them structure and support and water regulation. Plant cells have a central vacuole which regulates water and stores nutrients. Plant cells also have choloroplast which they use for energy from sunlight.
Here is the video that helped me understand this: http://www.youtube.com/watch?v=9exRxOozxtI
The purpose of this lab was to get an understanding of the different types of cells and their size. Some of these cells are visible to the human eye and some are only visible under a microscope. It also shows the function of specific cells and demonstrates how structures of a cell can be identified.
My observations include several different types of cells including bacteria like e coli and others.
Observations: Although a grain of rice or coffee bean is observable and recognizable to the human eye, mitochondria and E. coli bacteria are not. They are dramatically smaller in size yet dramatically larger in size when compared to single atoms.
It is interesting to understand how certain chemical substances or antibodies attach themselves to specific cell structures. This makes the organelles and other cellular structures like the cell wall observable and identifiable under a microscope.
Conclusion: Different organisms vastly differ in size. This is fascinating because it demonstrates that there is so much operating beyond the scope of human perception. Microscopes are extremely powerful in being able to identify different items, bacteria, and single cells/atoms. Chemical and antibody lacing or dying can be extremely useful in identifying types...