Thin-Layer Chromatography of Steroid Hormones

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Thin-Layer Chromatography of Steroid Hormones

Physiology Report 4.2
March 7th, 2012

By: Taryn Westerman
Donald Fru
Gloria Villarreal
Julian Garcia

Objective
The purpose of this experiment was to test the solubility of various steroids in order to obtain more information about their structure. An unknown was also given to be included in the experiment. The test that was performed was thin-layer chromatography which required a glass plate. The known steroids used in this experiment were estradiol, hydrocortisone, cortisone, corticosterone and deoxycorticosterone. Introduction

Steroid hormones are a subgroup of lipids. They are found in one of the primary endocrine glands in the body, the adrenal glands, which are located directly superior to the kidneys. The structures of the adrenal glands begin with the outer layer known as the cortex. This region is most commonly referred to as the adrenal cortex and provides the gland with approximately 80% of its total mass. Within the adrenal cortex there are three layers: the outermost layer is known as the zona glomerulosa, the middle layer is known as the zona fasciculata, and the innermost layer is known as the zona reticularis. Each of these layers is comprised of many cells that synthesize and secrete hormones appropriately named the adrenocorticoids. The second portion of the adrenal gland is known as the adrenal medulla. It is the inner region and account for the remaining 20% in each of the adrenal glands. The adrenal medulla is responsible for the release of epinephrine and norepinephrine into the bloodstream. Adrenocorticoids include three types of steroid hormones. Each class belongs to a specific layer in the adrenal cortex and each class secretes specific hormones. The first group of adrenocorticoids is referred to as mineralcorticoids. These hormones are secreted by the zona glomerulosa and are responsible for the regulation of both the reuptake of sodium and the secretion of potassium. The...
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