Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure
Chromatograph method is a method of separating mixtures of two or more compounds. Two phases are important in this method; one that is stationary and one that is moving. Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two different phases between which are to be partitioned. Thin Layer Chromatography (TLC) is a solid-liquid technique. The mixture is observed when it is in two different phase; a solid (stationary phase) and a liquid (moving phase). Thin Layer Chromatography (TLC) is a technique which is sensitive, cheap and fast. A thin layer chromatograph plate is used to identify drug component under UV. It is used to support the identity of a compound in a mixture when the Rf of a compound is compared with the Rf of a known compound. Results
Sample| Distance between start line and spot| Distance between start line and front line| Rf| Aspirin| 3.1 cm| 3.4 cm| 0.912|
Caffeine| 0.7 cm| 3.4 cm| 0.206|
Unknown X1)Unknown Component 12)Unknown Component 2| 1)3.1 cm2)0.7 cm| 1)3.4 cm2)3.4 cm| 1)0.9122)0.206|
Rf =distance traveled by the substance /distance traveled by the solvent front
By examining the functional groups of the analgesic drug components, the relative strength of their polarities can be determined. For example, if a compound contains carboxylic acid, alcohol or amine functional groups, it should be very polar. If a compound contains ketone, aldehyde, ester, amide, or ether functional groups, it should be polar. Lastly, if a compound...