Thin Layer Chromatography Lab

Only available on StudyMode
  • Download(s) : 280
  • Published : February 26, 2012
Open Document
Text Preview
Thin Layer Chromatography Lab
November 30, 2011

Calculations
Substance| Distance moved by Substance| Distance moved by Solvent| Rƒ| Methyl Orange| 3.4 cm| 4.5 cm| .75|
Methyl Violet| 3.6 cm| 4.5 cm| .80|
Indigo Carmine| 3.4 cm| 4.5 cm| .75|
Sudan IV| 4.6 cm| 5 cm| .92|
Methylene Blue| 3.7 cm| 5 cm| .74|
“Unknown” I| 4.1 cm| 4.5 cm| .91|
“Unknown” II| 4.1 cm| 4.5 cm| .91|

Methyl Orange moved with the solvent not leaving a trail. Instead the substance left a small, orange dot at its end place. Methyl Violet left a violet trail that started off small and gradually turned into a thick trail. Indigo Carmine also left a trail of blue but left a yellow dot at its end place. This substance’s trail is faded. Sudan IV, like Methyl Orange, did not leave a trail. Instead it left a dark orange dot, with a darker mark on top. Methylene Blue left a trail that only lasted for a few cm, unlike the rest. It is dark blue and flares out from a small trail to a thick trail.

Unknown I started as a blue trail that flares out from a small trail to a thick trail. This fades into a big purple dot that fades into yellow. Unknown II has a purple trail that starts small and eventually thickens. Substance| Distance moved by Substance| Distance moved by Solvent| Uncertainties| Methyl Orange| 3.4 cm| 4.5 cm| ±.05|

Methyl Violet| 3.6 cm| 4.5 cm| ±.05|
Indigo Carmine| 3.4 cm| 4.5 cm| ±.05|
Sudan IV| 4.6 cm| 5 cm| ±.05|
Methylene Blue| 3.7 cm| 5 cm| ±.05|
“Unknown” I| 4.1 cm| 4.5 cm| ±.05|
“Unknown” II| 4.1 cm| 4.5 cm| ±.05|

The uncertainty is ±.05 as the ruler used had the smallest increment of .1 cm, which divided by 2, is .05. Substance| Rƒ| Percent Uncertainties|
Methyl Orange| .75| 6.66 %|
Methyl Violet| .80| 6.25 %|
Indigo Carmine| .75| 6.66 %|
Sudan IV| .92| 5.43 %|
Methylene Blue| .74| 6.75 %|
“Unknown” I| .91| 5.49 %|
“Unknown” II| .91| 5.49 %|...
tracking img