The Relationship Between The Genotype And Phenotype Of Drosophila Melanogaster Eye Pigment
The Relationship between the Genotype and Phenotype of Drosophila melanogaster Eye Pigment
Mariam Rahmanyar
100486882
T.A: Zahra Mortaji
BIOL 2020
Results: Our TLC plate showed all colours expressed by the pteridine pigments for the wild-type Drosophila as expected (Figure 1). The sample A mutant which was a bright-red eye mutant also expressed the same pigments as our wild-type. Compared to the wild type, sample A pigment spots were lower intensity in colour under the UV light (Figure 2). Sample B was a brown-eye phenotype that showed no pigments except for a very faint 2-amino-4-hydroxypterin pigment (Figure 2). This was probably the result of human error because it was expected that there would be no pigments shown on the TLC plate for the brown-eye mutant. Furthermore, sample C which was the white-eyed mutant where no pigments were expected; however, our actual results did not reflect this. Instead our TLC plate faintly expressed Isoxanthopterin (violet-blue) for the white eye mutant (Figure 2). This was most likely due to human error. Moreover, the pigments found for sample D which was the dark-brown grey phenotype were: Isosepiapterin, Biopterin, 2-amino-4-hydroxypterin, and Isoxanthopterin and was lacking Sepiapterin, Xanthopterin, and Drosopterin (Figure 1). The Sepiapterin and Xanthopterin pigment was expected to show on the TLC plate (Figure 1) but unfortunately our plate did not reflect this. Again, this could be due to human error during the experiment. The Rf values for the pteridine pigment spots were similar for both the wild type and mutants of Drosophila. The wild type and sample A (bright-red eye mutant) had very similar Rf values for all the pigments (Table 1). Conversely, the Rf values for Isosepiapterin, Isoxanthopterin and Sepiapterin for the wild-type were slightly higher than those same pigments of sample A (Table 1). The 2-amino-4-hydroxypterin spot for sample B (Brown-eye) was close in value to the other
Mariam Rahmanyar
100486882
T.A: Zahra Mortaji
BIOL 2020
Results: Our TLC plate showed all colours expressed by the pteridine pigments for the wild-type Drosophila as expected (Figure 1). The sample A mutant which was a bright-red eye mutant also expressed the same pigments as our wild-type. Compared to the wild type, sample A pigment spots were lower intensity in colour under the UV light (Figure 2). Sample B was a brown-eye phenotype that showed no pigments except for a very faint 2-amino-4-hydroxypterin pigment (Figure 2). This was probably the result of human error because it was expected that there would be no pigments shown on the TLC plate for the brown-eye mutant. Furthermore, sample C which was the white-eyed mutant where no pigments were expected; however, our actual results did not reflect this. Instead our TLC plate faintly expressed Isoxanthopterin (violet-blue) for the white eye mutant (Figure 2). This was most likely due to human error. Moreover, the pigments found for sample D which was the dark-brown grey phenotype were: Isosepiapterin, Biopterin, 2-amino-4-hydroxypterin, and Isoxanthopterin and was lacking Sepiapterin, Xanthopterin, and Drosopterin (Figure 1). The Sepiapterin and Xanthopterin pigment was expected to show on the TLC plate (Figure 1) but unfortunately our plate did not reflect this. Again, this could be due to human error during the experiment. The Rf values for the pteridine pigment spots were similar for both the wild type and mutants of Drosophila. The wild type and sample A (bright-red eye mutant) had very similar Rf values for all the pigments (Table 1). Conversely, the Rf values for Isosepiapterin, Isoxanthopterin and Sepiapterin for the wild-type were slightly higher than those same pigments of sample A (Table 1). The 2-amino-4-hydroxypterin spot for sample B (Brown-eye) was close in value to the other